Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVT68_RS10140 Genome accession   NZ_CP118605
Coordinates   2437893..2438927 (+) Length   344 a.a.
NCBI ID   WP_280317690.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2432893..2443927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS10120 (PVT68_10120) - 2433167..2435143 (-) 1977 WP_280317682.1 dynamin family protein -
  PVT68_RS10125 (PVT68_10125) - 2435350..2435955 (-) 606 WP_280317684.1 YggT family protein -
  PVT68_RS10130 (PVT68_10130) proC 2436208..2437059 (-) 852 WP_280317686.1 pyrroline-5-carboxylate reductase -
  PVT68_RS10135 (PVT68_10135) - 2437111..2437806 (-) 696 WP_280317688.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVT68_RS10140 (PVT68_10140) pilT 2437893..2438927 (+) 1035 WP_280317690.1 type IV pilus twitching motility protein PilT Machinery gene
  PVT68_RS10145 (PVT68_10145) pilU 2438996..2440144 (+) 1149 WP_280317692.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT68_RS10150 (PVT68_10150) - 2440243..2440515 (+) 273 WP_280317694.1 hypothetical protein -
  PVT68_RS10155 (PVT68_10155) - 2440657..2442360 (+) 1704 WP_280317696.1 amidohydrolase -
  PVT68_RS10160 (PVT68_10160) - 2442375..2443139 (-) 765 WP_280317698.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38453.06 Da        Isoelectric Point: 6.6406

>NTDB_id=793915 PVT68_RS10140 WP_280317690.1 2437893..2438927(+) (pilT) [Microbulbifer bruguierae strain H12]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKHISDTPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
QELVEKRIISRESAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=793915 PVT68_RS10140 WP_280317690.1 2437893..2438927(+) (pilT) [Microbulbifer bruguierae strain H12]
ATGGACATTACAGAACTCCTCGCCTTTAGCGCCAAGCAGAACGCCTCCGACTTGCACCTCTCCGCCGGCCTGCCCCCAAT
GATCCGGGTCGACGGCGATGTGCGCCGTATCAACCTGCCGCCCATGGAGCACAAGCAGGTACACGGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTTCCTGGAAACCGACTTCTCCTTCGAAGTCCCTGGTGTAGCCCGC
TTCCGTGTGAATGCGTTCAACCACAACCGCGGCGCCGGCGCCGTATTCCGGACCATTCCGTCCAAGGTACTGACCATGGA
AGACCTGGGGATGGGCCAGGTGTTCAAACACATCTCCGACACCCCCCGCGGCCTGGTACTGGTCACCGGCCCCACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCGATGATCGACTACATCAACGACAACAAATACGAACACATCCTCACCATTGAGGAC
CCGATCGAATTCGTACACGAGTCGAAAAAGTGCCTGGTGAACCAGCGGGAAGTCCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCTGCCCTGCGTGAAGACCCCGACATCATCCTCGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGCCACCTGGTTTTCGGCACCCTGCACACCACCTCTGCTGCCAAAACCATCGACCGTGTG
GTAGACGTATTCCCGGCGCAGGAAAAATCCATGGTGCGCTCCATGCTGTCGGAATCGCTGCAGGCGGTTATTTCCCAGAC
CCTGCTCAAGCGCAACGGCGGCGGCCGCGTCGCCGCCCACGAAATCATGCGCGGCACCCCGGCCATCCGTAACCTGATCC
GTGAAGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGCGCCAGTGTAGGTATGCAGACCATGGACCAGTGCCTG
CAAGAACTGGTGGAAAAACGTATCATCAGCCGTGAATCGGCACGGGAAAAAGCGAAAATGCCGGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39