Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVT68_RS10145 Genome accession   NZ_CP118605
Coordinates   2438996..2440144 (+) Length   382 a.a.
NCBI ID   WP_280317692.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2433996..2445144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS10125 (PVT68_10125) - 2435350..2435955 (-) 606 WP_280317684.1 YggT family protein -
  PVT68_RS10130 (PVT68_10130) proC 2436208..2437059 (-) 852 WP_280317686.1 pyrroline-5-carboxylate reductase -
  PVT68_RS10135 (PVT68_10135) - 2437111..2437806 (-) 696 WP_280317688.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVT68_RS10140 (PVT68_10140) pilT 2437893..2438927 (+) 1035 WP_280317690.1 type IV pilus twitching motility protein PilT Machinery gene
  PVT68_RS10145 (PVT68_10145) pilU 2438996..2440144 (+) 1149 WP_280317692.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT68_RS10150 (PVT68_10150) - 2440243..2440515 (+) 273 WP_280317694.1 hypothetical protein -
  PVT68_RS10155 (PVT68_10155) - 2440657..2442360 (+) 1704 WP_280317696.1 amidohydrolase -
  PVT68_RS10160 (PVT68_10160) - 2442375..2443139 (-) 765 WP_280317698.1 LytTR family DNA-binding domain-containing protein -
  PVT68_RS10165 (PVT68_10165) - 2443136..2444353 (-) 1218 WP_280317699.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42657.93 Da        Isoelectric Point: 6.3736

>NTDB_id=793916 PVT68_RS10145 WP_280317692.1 2438996..2440144(+) (pilU) [Microbulbifer bruguierae strain H12]
MEIERLLQLVTEKGASDLFITAGVPASIKLHGKIVPVSTSSLTPEKAREMVVGIMNERQKKEFIETKELNFAISVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEQLKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTDSFETALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLRAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVHKMKELMKRSTEQGMQTFDQA
LYELYDRGEITYEDALSHADSANDLRLMIKLKSESDAEYLNSAADELSLASDSDSGSSPRIY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=793916 PVT68_RS10145 WP_280317692.1 2438996..2440144(+) (pilU) [Microbulbifer bruguierae strain H12]
ATGGAAATCGAACGACTCCTACAACTGGTGACTGAAAAAGGCGCATCGGATCTGTTCATCACCGCCGGTGTACCCGCCTC
CATCAAACTGCACGGCAAGATCGTTCCTGTAAGCACCTCCTCCCTGACGCCGGAAAAAGCTCGGGAAATGGTCGTCGGCA
TCATGAACGAGCGGCAGAAAAAAGAGTTCATCGAAACCAAAGAACTCAACTTCGCCATCTCCGTGCGCAACGTCGGCCGC
TTCCGGGTCTCCGCCTTCTTCCAGCGCAACCTCGTCGGCATGGTATTGCGTCGTATCGAAACCAAAATTCCCACCATCGA
CGGCCTCGGATTGCCGGAACAGCTCAAAGAGCTGGCGATGACCAAGCGCGGGCTGATCATCTTCGTGGGCGCCACCGGTA
CCGGCAAATCCACCTCGCTCGCCTCCATGATCGGTCACCGCAACGAGAACTCGAAGGGTCACATCATCTCCATCGAAGAC
CCGATCGAATTTATTCACCAGCACCGGGGCTGTATCGTGACCCAGCGCGAAGTGGGGCTCGATACCGACTCCTTCGAAAC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTCATCCTGATCGGTGAGGTGCGCTCCCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTGTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATTCACTTCTTCCCCGCCGACCGGCACCAGCAGTTGTGGATGGACCTCTCCCTCAACCTGCGCGCCATGGTCGCCCAGCA
GCTGGTGCCGACACCGGATGGCGACGGCCGCCGCGCCTGTCTCGAAATCATGATCAACACCCCGCTGATGTCGGATCTGA
TCCGCAAAGGTGAAGTGCACAAAATGAAGGAGCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGACCGCGGCGAAATCACCTACGAAGACGCCCTGTCACATGCCGACTCCGCCAACGACTTGCGCCT
GATGATCAAGCTCAAGTCCGAGTCCGACGCCGAGTACCTCAACAGCGCCGCCGACGAACTCAGTCTCGCCAGCGATAGCG
ACAGCGGTAGTAGCCCGCGTATTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(65-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.328

99.738

0.751
  pilU Acinetobacter baylyi ADP1

67.507

93.455

0.631
  pilU Vibrio cholerae strain A1552

55.337

93.194

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

88.743

0.393
  pilT Legionella pneumophila strain ERS1305867

41.791

87.696

0.366
  pilT Legionella pneumophila strain Lp02

41.791

87.696

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.95

88.22

0.361
  pilT Vibrio cholerae strain A1552

40.95

88.22

0.361
  pilT Acinetobacter nosocomialis M2

40.708

88.743

0.361
  pilT Acinetobacter baumannii D1279779

40.708

88.743

0.361
  pilT Acinetobacter baumannii strain A118

40.708

88.743

0.361