Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVK79_RS20655 Genome accession   NZ_CP118496
Coordinates   4778587..4779627 (-) Length   346 a.a.
NCBI ID   WP_145234931.1    Uniprot ID   -
Organism   Pseudoalteromonas rhizosphaerae strain hCg-42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4773587..4784627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK79_RS20625 rsmE 4773751..4774485 (+) 735 WP_145234944.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PVK79_RS20630 gshB 4774540..4775493 (+) 954 WP_145234942.1 glutathione synthase -
  PVK79_RS20635 - 4775521..4776078 (+) 558 WP_145234940.1 YqgE/AlgH family protein -
  PVK79_RS20640 ruvX 4776115..4776561 (+) 447 WP_145234938.1 Holliday junction resolvase RuvX -
  PVK79_RS20645 - 4776524..4777435 (-) 912 WP_145234935.1 hypothetical protein -
  PVK79_RS20650 pilU 4777437..4778567 (-) 1131 WP_145234933.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVK79_RS20655 pilT 4778587..4779627 (-) 1041 WP_145234931.1 type IV pilus twitching motility protein PilT Machinery gene
  PVK79_RS20660 - 4779630..4780343 (+) 714 WP_274803963.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVK79_RS20665 proC 4780359..4781180 (+) 822 WP_257324430.1 pyrroline-5-carboxylate reductase -
  PVK79_RS20670 - 4781238..4781771 (+) 534 WP_149981274.1 YggT family protein -
  PVK79_RS20675 - 4781823..4782266 (+) 444 WP_257324432.1 DUF4426 domain-containing protein -
  PVK79_RS20680 - 4782270..4782869 (+) 600 WP_145234920.1 XTP/dITP diphosphatase -
  PVK79_RS20685 hemW 4782866..4784002 (+) 1137 WP_182731341.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38204.48 Da        Isoelectric Point: 6.4495

>NTDB_id=792845 PVK79_RS20655 WP_145234931.1 4778587..4779627(-) (pilT) [Pseudoalteromonas rhizosphaerae strain hCg-42]
MDITELLAFSVQHKASDLHLSSGVAPMIRVDGDVRRINIPALENKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDHINQHKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
INLVNHGVVTNAEARAKAQDKNNFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=792845 PVK79_RS20655 WP_145234931.1 4778587..4779627(-) (pilT) [Pseudoalteromonas rhizosphaerae strain hCg-42]
ATGGATATTACCGAATTGTTGGCCTTTAGTGTGCAGCACAAAGCATCAGATTTACACTTATCATCAGGGGTTGCGCCAAT
GATCCGTGTTGATGGTGATGTGCGACGTATTAATATACCAGCGCTGGAAAACAAAGATGTTAACAGCCTAGTTTACGATA
TTATGAACGATAATCAACGCAAGGACTATGAACAAAACCTTGAAGTGGATTTTTCGTTTGAAGTGCCAAACTTAGCACGT
TTTCGTGTTAATGCCTTCAACTCAAATCGAGGCCCCGCAGCTGTATTTCGTACCATTCCCAGTGAAGTGCTGACGCTTGA
TGATTTAGGTGCGCCAGATATATTTAAAACAATCTCAGACACGCCGCGTGGTTTAGTGTTGGTAACAGGACCTACAGGCT
CTGGTAAATCGACCACATTGGCTGCGATGGTGGATCATATAAATCAACACAAGCATCATCATATTTTAACCATCGAAGAT
CCCATCGAGTTTGTGCACGACAATAAACTGTCGTTGATTAATCAACGTGAAGTGCATCGCGATACGCATAGTTTTTCTAA
TGCATTGCGCAGTGCTTTACGTGAAGATCCAGACGTAATTTTGGTTGGTGAGCTACGTGATTTAGAAACTATTCGCCTTG
CCATGACTGCTGCTGAAACCGGCCATTTAGTATTTGGTACATTGCATACTACGTCAGCCCCGAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTGAAGAAAAAAGTATGATCCGCTCAATGCTTTCAGAGTCATTACGCGCAGTTATTTCACAAAC
CCTGTTGAAAAAAATTGGTGGCGGGCGAGTCGCAGCACATGAAATCATGATTGCAGTGCCTGCGATCCGTAACCTTATCC
GCGAAGATAAAATTGCGCAAATGTATTCGTCCATTCAAACTGGCGCATCGCATGGTATGCAAACCATGGATCAGTGTTTA
ATCAATTTAGTGAATCACGGTGTTGTTACCAATGCTGAAGCGCGTGCTAAAGCACAAGATAAAAATAATTTCGGCGGCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Pseudomonas stutzeri DSM 10701

75.872

99.422

0.754
  pilT Pseudomonas aeruginosa PAK

75.581

99.422

0.751
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria meningitidis 8013

68.986

99.711

0.688
  pilT Neisseria gonorrhoeae MS11

68.696

99.711

0.685
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.711

0.523
  pilU Vibrio cholerae strain A1552

40.58

99.711

0.405
  pilU Acinetobacter baylyi ADP1

39.884

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.551

99.711

0.384