Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVK79_RS20650 Genome accession   NZ_CP118496
Coordinates   4777437..4778567 (-) Length   376 a.a.
NCBI ID   WP_145234933.1    Uniprot ID   -
Organism   Pseudoalteromonas rhizosphaerae strain hCg-42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4772437..4783567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK79_RS20620 - 4773172..4773645 (+) 474 WP_145234946.1 hypothetical protein -
  PVK79_RS20625 rsmE 4773751..4774485 (+) 735 WP_145234944.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PVK79_RS20630 gshB 4774540..4775493 (+) 954 WP_145234942.1 glutathione synthase -
  PVK79_RS20635 - 4775521..4776078 (+) 558 WP_145234940.1 YqgE/AlgH family protein -
  PVK79_RS20640 ruvX 4776115..4776561 (+) 447 WP_145234938.1 Holliday junction resolvase RuvX -
  PVK79_RS20645 - 4776524..4777435 (-) 912 WP_145234935.1 hypothetical protein -
  PVK79_RS20650 pilU 4777437..4778567 (-) 1131 WP_145234933.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVK79_RS20655 pilT 4778587..4779627 (-) 1041 WP_145234931.1 type IV pilus twitching motility protein PilT Machinery gene
  PVK79_RS20660 - 4779630..4780343 (+) 714 WP_274803963.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVK79_RS20665 proC 4780359..4781180 (+) 822 WP_257324430.1 pyrroline-5-carboxylate reductase -
  PVK79_RS20670 - 4781238..4781771 (+) 534 WP_149981274.1 YggT family protein -
  PVK79_RS20675 - 4781823..4782266 (+) 444 WP_257324432.1 DUF4426 domain-containing protein -
  PVK79_RS20680 - 4782270..4782869 (+) 600 WP_145234920.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41625.67 Da        Isoelectric Point: 6.0925

>NTDB_id=792844 PVK79_RS20650 WP_145234933.1 4777437..4778567(-) (pilU) [Pseudoalteromonas rhizosphaerae strain hCg-42]
MTHSLDYFLHLMIEKQGSDLFVSSQLPVSAKINGELIALGDEKISDEESLQLVESAMSEKQKAEFHNTKECNFAIATDEG
RFRISAFWQRDCAGMVIRRIVTQIPDVAELGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFEAALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLSLNLRAIIAQQLVPTADGNGRVAAIEILLNTPIIGELIKKGDIGGIKEAMAKAKDMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLEPKGQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=792844 PVK79_RS20650 WP_145234933.1 4777437..4778567(-) (pilU) [Pseudoalteromonas rhizosphaerae strain hCg-42]
ATGACACATTCTCTTGATTACTTTTTACATTTGATGATTGAAAAGCAAGGTTCGGATTTATTCGTATCGAGCCAATTGCC
AGTCAGCGCAAAGATTAATGGCGAACTAATTGCACTTGGCGACGAAAAAATATCAGACGAAGAGTCGCTGCAATTAGTTG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAATACTAAAGAGTGCAACTTTGCCATAGCTACCGATGAAGGG
CGCTTTAGGATCTCGGCATTTTGGCAACGAGATTGCGCGGGTATGGTTATTCGCCGTATTGTTACGCAAATTCCTGATGT
TGCTGAGCTTGGTCTACCGTCGACATTGACCGATGTGATCATGGCTAAACGTGGTTTAGTGCTGTTTGTCGGTGGTACTG
GCACCGGTAAATCAACGTCGTTGGCTGCATTATTAGGTTACCGTAATCGTAATCAACGTGGGCATATCTTGACCATTGAA
GACCCGATTGAGTTTGTCCATGAACATCGCAAAAGTATTATTACTCAGCGTGAAGTTGGCTTAGATACGGAAAGTTTTGA
GGCGGCGTTAAAAAGCTCATTACGCCAAGCCCCCGATGTCATTTTAATTGGTGAAATACGCTCGCAAGAGACCATGGAAT
ACGCACTTAGTTTTGCTGAAACCGGTCATTTATGTGTTGCTACATTACATGCCAATAACGCTAACCAAGCAATTGACCGT
ATTATGCATTTAGTGCCTAAAGAAAAGCACGATAAGCTAAAATATGATCTATCCTTAAACTTACGAGCTATCATAGCACA
ACAACTAGTGCCTACAGCTGATGGCAATGGTCGAGTAGCTGCTATCGAAATTTTATTGAATACCCCGATTATCGGCGAGC
TTATCAAAAAAGGCGACATTGGCGGCATTAAAGAAGCCATGGCTAAAGCGAAAGATATGGGTATGCAAACCTTTGATCAA
GCGTTATTTGAGCTCTACAAACAGCAACGTATCAACTATGCAGATGCTTTGCATCACGCTGACTCACCTAACGATTTACG
TTTGATGATTAAGCTTCGAAATAATGAACAGCAAGGGGCTGGTTTTTTACAGGGAGTAACAATTGACGGGTTAGAACCTA
AAGGTCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.599

92.819

0.553
  pilU Acinetobacter baylyi ADP1

55.645

98.936

0.551
  pilU Vibrio cholerae strain A1552

56.438

97.074

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.681

96.809

0.423
  pilT Acinetobacter baylyi ADP1

40.653

89.628

0.364