Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NIL_RS03080 Genome accession   NZ_AP022826
Coordinates   615176..616243 (-) Length   355 a.a.
NCBI ID   WP_281389206.1    Uniprot ID   -
Organism   Nitrosophilus labii strain HRV44     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 610176..621243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIL_RS03060 - 610787..611503 (-) 717 WP_187648154.1 SPOR domain-containing protein -
  NIL_RS03065 - 611611..612858 (-) 1248 WP_187648155.1 serine hydroxymethyltransferase -
  NIL_RS03070 lysS 612855..614375 (-) 1521 WP_187648156.1 lysine--tRNA ligase -
  NIL_RS03075 - 614389..615012 (-) 624 WP_187648157.1 CvpA family protein -
  NIL_RS03080 pilT 615176..616243 (-) 1068 WP_281389206.1 type IV pilus twitching motility protein PilT Machinery gene
  NIL_RS03085 gatC 616257..616547 (-) 291 WP_187648158.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  NIL_RS03090 - 616696..617301 (+) 606 WP_187648159.1 hypothetical protein -
  NIL_RS03095 - 617301..618305 (+) 1005 WP_187648160.1 hypothetical protein -
  NIL_RS03100 - 618302..618634 (+) 333 WP_187648161.1 hypothetical protein -
  NIL_RS03105 - 618621..619241 (+) 621 WP_187648162.1 type III pantothenate kinase -
  NIL_RS03110 hisG 619235..619849 (+) 615 WP_187648163.1 ATP phosphoribosyltransferase -
  NIL_RS03115 - 619852..620535 (+) 684 WP_187648164.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39587.80 Da        Isoelectric Point: 6.9838

>NTDB_id=79266 NIL_RS03080 WP_281389206.1 615176..616243(-) (pilT) [Nitrosophilus labii strain HRV44]
MSELDIRKLLKSVIAYKASDLHLVSGSEPQIRIDGKLIPLNLPKLDGKQIEEMCYGIITDKQKKIFEEEGELDFALMVPN
VGRFRVNYYTTLGDIAAAFRIIPLKVPTLEDINAPEIFYQIIKREKGLILVTGPTGSGKSTTLAAMLNEINTHERKHILT
IEDPVEFVHENKKSLFSHREVGSDTKSFAIALKYALREDPDIILIGEMRDKETISAALTAAETGHLVLGTLHTNSASQTV
NRIIDVFSGDEQPQVRAMLSTSLVAVISQALIPKIGGGRVATFEILINTPAIANLIRENKTHQIYSQMQLNQQQTGMQTQ
TQVILKLLKNHVISKEDALRYSNRPEELKNLIQAL

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=79266 NIL_RS03080 WP_281389206.1 615176..616243(-) (pilT) [Nitrosophilus labii strain HRV44]
ATGAGTGAGTTGGACATAAGAAAACTTTTAAAAAGTGTTATAGCTTATAAAGCCTCAGATCTACACTTGGTTAGCGGAAG
CGAACCACAAATAAGAATCGACGGCAAACTGATTCCTTTAAATCTTCCTAAGTTAGACGGTAAACAGATTGAGGAGATGT
GTTACGGCATAATTACGGATAAACAGAAAAAGATTTTCGAAGAAGAGGGTGAGCTGGATTTTGCTTTAATGGTGCCAAAT
GTTGGAAGATTCAGAGTAAACTACTACACTACTTTAGGTGATATTGCAGCCGCATTTAGGATAATTCCTCTAAAAGTTCC
AACACTAGAAGATATAAACGCCCCTGAAATCTTTTATCAGATAATCAAAAGGGAAAAAGGTCTAATCCTAGTAACCGGAC
CTACAGGAAGCGGTAAATCAACTACTCTAGCTGCCATGTTAAACGAGATAAATACACATGAAAGAAAACATATCCTAACA
ATTGAAGATCCTGTAGAGTTTGTTCATGAAAATAAAAAATCACTATTTTCCCACAGAGAAGTAGGCTCAGATACAAAATC
TTTTGCAATCGCTTTGAAATATGCGTTGCGGGAAGATCCAGATATTATATTGATCGGTGAGATGAGGGATAAAGAGACTA
TCTCAGCGGCATTAACGGCGGCGGAAACAGGTCATTTGGTTCTTGGAACTTTGCATACAAACTCGGCATCTCAAACCGTA
AACAGAATTATTGACGTATTTAGCGGAGATGAACAGCCTCAAGTAAGAGCTATGCTCTCAACAAGTTTAGTCGCGGTTAT
CTCTCAAGCGCTTATACCAAAAATAGGTGGGGGAAGAGTAGCAACATTTGAAATTCTTATTAATACTCCAGCAATTGCAA
ACCTAATAAGGGAAAACAAAACTCACCAGATCTATTCACAAATGCAACTAAACCAACAACAAACGGGAATGCAAACACAA
ACGCAAGTTATTTTAAAACTGCTTAAAAATCATGTAATTTCAAAAGAAGATGCATTAAGATATTCCAATAGGCCCGAAGA
ACTAAAAAATCTCATCCAGGCTTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

55.362

97.183

0.538
  pilT Pseudomonas aeruginosa PAK

54.783

97.183

0.532
  pilT Acinetobacter baumannii strain A118

54.493

97.183

0.53
  pilT Acinetobacter nosocomialis M2

54.493

97.183

0.53
  pilT Acinetobacter baumannii D1279779

54.493

97.183

0.53
  pilT Acinetobacter baylyi ADP1

53.913

97.183

0.524
  pilT Neisseria gonorrhoeae MS11

53.179

97.465

0.518
  pilT Neisseria meningitidis 8013

52.89

97.465

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.79

94.085

0.515
  pilT Vibrio cholerae strain A1552

54.79

94.085

0.515
  pilT Legionella pneumophila strain Lp02

53.134

94.366

0.501
  pilT Legionella pneumophila strain ERS1305867

53.134

94.366

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.154

98.873

0.456
  pilU Acinetobacter baylyi ADP1

41.667

98.028

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.873

0.386
  pilU Vibrio cholerae strain A1552

39.031

98.873

0.386

Multiple sequence alignment