Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVT67_RS02725 Genome accession   NZ_CP118480
Coordinates   575903..576937 (+) Length   344 a.a.
NCBI ID   WP_301497560.1    Uniprot ID   -
Organism   Gallaecimonas kandeliae strain Q10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570903..581937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT67_RS02690 (PVT67_02690) hemW 571243..572370 (-) 1128 WP_301499630.1 radical SAM family heme chaperone HemW -
  PVT67_RS02695 (PVT67_02695) - 572375..572965 (-) 591 WP_301497548.1 XTP/dITP diphosphatase -
  PVT67_RS02700 (PVT67_02700) - 573018..573449 (-) 432 WP_301497550.1 DUF4426 domain-containing protein -
  PVT67_RS02705 (PVT67_02705) yggU 573513..573800 (-) 288 WP_301497552.1 DUF167 family protein YggU -
  PVT67_RS02710 (PVT67_02710) - 573797..574345 (-) 549 WP_301497554.1 YggT family protein -
  PVT67_RS02715 (PVT67_02715) proC 574345..575160 (-) 816 WP_301497556.1 pyrroline-5-carboxylate reductase -
  PVT67_RS02720 (PVT67_02720) - 575172..575870 (-) 699 WP_301497558.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVT67_RS02725 (PVT67_02725) pilT 575903..576937 (+) 1035 WP_301497560.1 type IV pilus twitching motility protein PilT Machinery gene
  PVT67_RS02730 (PVT67_02730) pilU 576948..578060 (+) 1113 WP_301497563.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT67_RS02735 (PVT67_02735) ruvX 578108..578524 (-) 417 WP_419181024.1 Holliday junction resolvase RuvX -
  PVT67_RS02740 (PVT67_02740) - 578524..579099 (-) 576 WP_301497565.1 YqgE/AlgH family protein -
  PVT67_RS02745 (PVT67_02745) gshB 579198..580130 (-) 933 WP_301497568.1 glutathione synthase -
  PVT67_RS02750 (PVT67_02750) rsmE 580140..580868 (-) 729 WP_301497570.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PVT67_RS02755 (PVT67_02755) - 580868..581569 (-) 702 WP_301497572.1 endonuclease -
  PVT67_RS02760 (PVT67_02760) - 581559..581723 (+) 165 WP_301497574.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38149.77 Da        Isoelectric Point: 7.1510

>NTDB_id=792620 PVT67_RS02725 WP_301497560.1 575903..576937(+) (pilT) [Gallaecimonas kandeliae strain Q10]
MDITELLAFSVKHNASDLHISAGVPPMIRVDGDVRRINLPALEHKEVHALIYDIMNDKQRKDYEETLEVDFSFEVPGVAR
FRVNAYNQNRGAAAVFRTIPSKVLTLDQLGAPAIFKDIVTKPRGLVLVTGPTGSGKSTTLAAMIDYVNDNRHDHILTIED
PIEFVHENKKCLINQREVHRDTHSFSNALRSALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKSMVRSMLSESLQAVISQTLLKKTGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGMTHGMQTLDQCL
KELVSRNQINRLDARAKAVDKNAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=792620 PVT67_RS02725 WP_301497560.1 575903..576937(+) (pilT) [Gallaecimonas kandeliae strain Q10]
ATGGATATTACCGAACTCCTGGCGTTCAGCGTCAAGCATAATGCCTCTGACCTGCACATCTCTGCGGGCGTGCCGCCCAT
GATCCGTGTTGACGGCGACGTGCGCCGTATCAACCTGCCTGCCTTGGAACACAAGGAAGTGCATGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTACGAGGAAACCCTGGAAGTGGACTTCTCCTTCGAAGTCCCCGGCGTGGCCCGT
TTCCGTGTCAACGCCTACAACCAGAACCGCGGCGCCGCCGCCGTGTTCAGGACCATCCCTTCCAAGGTGCTGACGCTGGA
CCAACTGGGCGCCCCGGCCATCTTCAAGGACATCGTCACCAAGCCGCGTGGCCTGGTGCTGGTGACGGGCCCCACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCCATGATCGACTATGTCAACGACAACCGCCACGACCATATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCATGAAAACAAGAAGTGCCTGATCAACCAGCGGGAAGTACACCGCGACACCCACAGCTTCTCCAA
CGCCCTGCGCTCGGCGCTCCGTGAAGACCCGGACGTGGTGCTAGTCGGTGAAATGCGGGACTTGGAAACCATCCGCCTGG
CCCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCTGCCGGTGAGAAATCCATGGTTCGTTCCATGCTGTCCGAATCCCTGCAGGCGGTCATCTCCCAGAC
GCTGCTGAAGAAGACCGGCGGCGGCCGGATCGCGGCCCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTTATCC
GCGAGGACAAGGTGGCCCAGATGTATTCCGCCATCCAGACCGGTATGACCCACGGCATGCAGACCCTGGACCAGTGCCTC
AAGGAACTGGTCAGCCGCAACCAGATCAACCGCTTGGATGCCCGCGCCAAGGCGGTAGACAAGAACGCCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.395

100

0.814
  pilT Acinetobacter baumannii strain A118

81.395

100

0.814
  pilT Pseudomonas aeruginosa PAK

82.249

98.256

0.808
  pilT Pseudomonas stutzeri DSM 10701

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

73.837

100

0.738
  pilT Legionella pneumophila strain ERS1305867

73.837

100

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.464

100

0.727
  pilT Vibrio cholerae strain A1552

72.464

100

0.727
  pilT Neisseria meningitidis 8013

68.732

98.547

0.677
  pilT Neisseria gonorrhoeae MS11

68.437

98.547

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Acinetobacter baylyi ADP1

41.279

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.816

99.709

0.407
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404