Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVT67_RS02730 Genome accession   NZ_CP118480
Coordinates   576948..578060 (+) Length   370 a.a.
NCBI ID   WP_301497563.1    Uniprot ID   -
Organism   Gallaecimonas kandeliae strain Q10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 571948..583060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT67_RS02695 (PVT67_02695) - 572375..572965 (-) 591 WP_301497548.1 XTP/dITP diphosphatase -
  PVT67_RS02700 (PVT67_02700) - 573018..573449 (-) 432 WP_301497550.1 DUF4426 domain-containing protein -
  PVT67_RS02705 (PVT67_02705) yggU 573513..573800 (-) 288 WP_301497552.1 DUF167 family protein YggU -
  PVT67_RS02710 (PVT67_02710) - 573797..574345 (-) 549 WP_301497554.1 YggT family protein -
  PVT67_RS02715 (PVT67_02715) proC 574345..575160 (-) 816 WP_301497556.1 pyrroline-5-carboxylate reductase -
  PVT67_RS02720 (PVT67_02720) - 575172..575870 (-) 699 WP_301497558.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVT67_RS02725 (PVT67_02725) pilT 575903..576937 (+) 1035 WP_301497560.1 type IV pilus twitching motility protein PilT Machinery gene
  PVT67_RS02730 (PVT67_02730) pilU 576948..578060 (+) 1113 WP_301497563.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT67_RS02735 (PVT67_02735) ruvX 578108..578524 (-) 417 WP_419181024.1 Holliday junction resolvase RuvX -
  PVT67_RS02740 (PVT67_02740) - 578524..579099 (-) 576 WP_301497565.1 YqgE/AlgH family protein -
  PVT67_RS02745 (PVT67_02745) gshB 579198..580130 (-) 933 WP_301497568.1 glutathione synthase -
  PVT67_RS02750 (PVT67_02750) rsmE 580140..580868 (-) 729 WP_301497570.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PVT67_RS02755 (PVT67_02755) - 580868..581569 (-) 702 WP_301497572.1 endonuclease -
  PVT67_RS02760 (PVT67_02760) - 581559..581723 (+) 165 WP_301497574.1 hypothetical protein -
  PVT67_RS02765 (PVT67_02765) - 581720..582310 (+) 591 WP_301497576.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41249.53 Da        Isoelectric Point: 6.6907

>NTDB_id=792621 PVT67_RS02730 WP_301497563.1 576948..578060(+) (pilU) [Gallaecimonas kandeliae strain Q10]
MDMKSLLQRVCELNASDLFITSGLPPCVKVNGEIRPVADQRLTAAEAKAMVESVMNEKQLAQYHEHKDANFAIAAKGIGR
FRVSAFVQRDADGMVLRRIQTQIPTIEELGLPPILKDIVMTKRGLVLMVGATGTGKSTSLAAMIGYRNENSRGHIVTIED
PIEFVHEHKRSIITQREVGIDCDSFDVALKNSLRQAPDVILLGEIRSQETMEFALAFAETGHLCMATLHANNANQALERV
LHLVPKEKHDQFLFDMSLNLKAVVAQQLIPKRDGTGRVVATEVLLHSPLVSEQIRKGDIHLLKDTMERSRELGMMTFDRS
LFELYAHGDITYADALHYADSPNDLRLMIKLSQNETGKTTSLDNVTIDKR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=792621 PVT67_RS02730 WP_301497563.1 576948..578060(+) (pilU) [Gallaecimonas kandeliae strain Q10]
ATGGACATGAAAAGCCTGCTGCAACGCGTCTGCGAACTGAACGCTTCGGATCTCTTCATCACCTCTGGGCTGCCGCCCTG
CGTCAAGGTCAACGGCGAGATCCGCCCGGTGGCGGACCAGCGCCTGACGGCGGCCGAGGCCAAGGCCATGGTGGAGAGCG
TCATGAACGAGAAGCAGCTGGCGCAATACCATGAACACAAGGACGCCAACTTCGCCATCGCCGCCAAGGGCATAGGCCGC
TTCCGGGTGTCGGCCTTCGTCCAGCGTGACGCTGACGGCATGGTGCTGCGCCGCATCCAGACCCAGATCCCCACCATAGA
GGAACTGGGGCTGCCCCCCATCCTCAAGGACATCGTCATGACCAAGCGCGGCCTGGTGTTGATGGTGGGGGCCACAGGTA
CGGGTAAGTCCACCTCCCTGGCGGCCATGATCGGCTATCGCAACGAGAACAGCCGCGGCCACATCGTCACCATCGAGGAC
CCCATCGAATTCGTCCATGAGCACAAGCGCAGCATCATCACCCAAAGGGAGGTGGGCATCGACTGTGACTCCTTCGATGT
GGCCCTGAAGAACAGCCTGCGCCAGGCCCCTGACGTGATACTGCTGGGGGAGATCCGCAGCCAGGAGACCATGGAGTTCG
CCCTGGCCTTCGCCGAGACGGGCCACCTCTGTATGGCCACACTGCACGCCAACAACGCCAACCAGGCGTTGGAGCGGGTG
TTGCACCTGGTGCCCAAGGAAAAGCACGACCAGTTCCTGTTCGACATGAGCCTCAACCTCAAGGCGGTGGTGGCCCAGCA
GTTGATCCCGAAAAGGGACGGCACAGGCCGGGTGGTGGCTACAGAGGTACTGCTGCATTCGCCCCTGGTGTCTGAGCAGA
TCCGCAAGGGCGACATCCATCTCCTCAAGGACACCATGGAGCGTTCCCGTGAGCTGGGCATGATGACCTTCGACCGCAGC
CTGTTCGAGCTCTATGCCCACGGCGATATCACCTATGCCGACGCCCTGCACTATGCCGACTCCCCCAACGATCTGCGCCT
GATGATCAAGCTCAGCCAGAACGAGACCGGCAAGACCACCTCCCTCGACAACGTCACCATCGACAAGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.732

96.757

0.597
  pilU Acinetobacter baylyi ADP1

58.904

98.649

0.581
  pilU Vibrio cholerae strain A1552

57.337

99.459

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.768

93.243

0.408
  pilT Acinetobacter baumannii strain A118

41.228

92.432

0.381
  pilT Acinetobacter baylyi ADP1

41.228

92.432

0.381
  pilT Acinetobacter baumannii D1279779

41.228

92.432

0.381
  pilT Acinetobacter nosocomialis M2

40.936

92.432

0.378
  pilT Pseudomonas aeruginosa PAK

39.71

93.243

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.42

93.243

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.854

88.649

0.362
  pilT Vibrio cholerae strain A1552

40.854

88.649

0.362