Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PU634_RS01430 Genome accession   NZ_CP118224
Coordinates   297132..298166 (+) Length   344 a.a.
NCBI ID   WP_306762306.1    Uniprot ID   A0AA50KNG0
Organism   Oceanimonas pelagia strain NTOU-MSR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292132..303166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PU634_RS01395 (PU634_01395) hemW 292295..293440 (-) 1146 WP_306762299.1 radical SAM family heme chaperone HemW -
  PU634_RS01400 (PU634_01400) rdgB 293440..294039 (-) 600 WP_306762300.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PU634_RS01405 (PU634_01405) - 294251..294664 (-) 414 WP_306762301.1 DUF4426 domain-containing protein -
  PU634_RS01410 (PU634_01410) - 294703..295014 (-) 312 WP_306762302.1 DUF167 family protein -
  PU634_RS01415 (PU634_01415) - 295002..295553 (-) 552 WP_306762303.1 YggT family protein -
  PU634_RS01420 (PU634_01420) proC 295563..296387 (-) 825 WP_306762304.1 pyrroline-5-carboxylate reductase -
  PU634_RS01425 (PU634_01425) - 296398..297090 (-) 693 WP_306762305.1 YggS family pyridoxal phosphate-dependent enzyme -
  PU634_RS01430 (PU634_01430) pilT 297132..298166 (+) 1035 WP_306762306.1 type IV pilus twitching motility protein PilT Machinery gene
  PU634_RS01435 (PU634_01435) pilU 298187..299296 (+) 1110 WP_306762307.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PU634_RS01440 (PU634_01440) yaaA 299304..300083 (+) 780 WP_306762308.1 peroxide stress protein YaaA -
  PU634_RS01445 (PU634_01445) - 300242..300391 (-) 150 WP_306762309.1 hypothetical protein -
  PU634_RS01450 (PU634_01450) srmB 300442..301740 (-) 1299 WP_306762310.1 ATP-dependent RNA helicase SrmB -
  PU634_RS01455 (PU634_01455) - 301811..302518 (+) 708 WP_306762311.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38228.80 Da        Isoelectric Point: 6.7438

>NTDB_id=791097 PU634_RS01430 WP_306762306.1 297132..298166(+) (pilT) [Oceanimonas pelagia strain NTOU-MSR1]
MDITELLAFSVKHNASDLHLSAGVQPLLRVDGEVRKINLPVLDHREVHRLVYDIMNDRQRKELEENFEVDFSFELPGLAR
FRVNAYHQARGAAAVFRVIPSEVWTLEQLGGPEIFERIAEYSRGLVLVTGPTGSGKSTTLAAMVNHINDNFNKHILTIED
PIEFVHDNKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQNLLKKINGGRVAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMAHGMQTMDQSL
KRLVAGGLVSPADARAKAVDPQSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=791097 PU634_RS01430 WP_306762306.1 297132..298166(+) (pilT) [Oceanimonas pelagia strain NTOU-MSR1]
ATGGACATTACCGAATTACTGGCGTTCAGTGTAAAGCATAATGCCTCCGATCTGCACCTCTCCGCCGGCGTGCAGCCGCT
GTTGCGGGTGGACGGCGAGGTGCGCAAGATCAACCTGCCGGTGCTGGATCACCGGGAGGTGCACCGGCTGGTGTACGACA
TCATGAATGATCGCCAGCGCAAGGAGCTGGAAGAAAACTTCGAGGTCGACTTTTCCTTTGAGCTGCCGGGGCTGGCGCGC
TTTCGGGTGAACGCCTACCACCAGGCCCGGGGCGCGGCGGCGGTATTCCGGGTGATCCCGAGCGAGGTGTGGACCCTGGA
GCAGCTGGGTGGCCCGGAGATCTTTGAGCGCATTGCCGAATATTCCCGTGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAATCACATCAACGACAACTTCAACAAGCACATTCTGACCATCGAGGAT
CCCATCGAATTTGTGCACGACAACAAGAAGTGCCTGGTCAACCAGCGGGAAGTGCACCGCGATACCCACTCCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGATCCCGACATCATTCTGGTGGGCGAACTGCGGGATCTGGAAACCATTCGGCTGG
CGCTTACCGCCGCTGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGATGTGTTTCCCGGTGCCGAAAAAGACATGGTGCGTTCCATGCTGTCGGAGTCGCTGCGGGCGGTGATCTCCCAAAA
CCTGCTGAAAAAGATAAACGGCGGCCGGGTGGCGGCTCACGAGATCATGCTGGGTACCCCGGCCATTCGCAACCTGATCC
GGGAAGACAAGGTGGCGCAGATGTATTCGGTGATCCAGACCGGCATGGCCCACGGCATGCAGACCATGGATCAGTCGCTG
AAGCGGCTGGTGGCCGGTGGTCTGGTATCGCCCGCCGACGCCCGTGCCAAGGCGGTGGATCCCCAGAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.872

100

0.759
  pilT Acinetobacter baumannii strain A118

75.872

100

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.151

97.965

0.756
  pilT Vibrio cholerae strain A1552

77.151

97.965

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baylyi ADP1

75

100

0.75
  pilT Pseudomonas aeruginosa PAK

74.709

100

0.747
  pilT Pseudomonas stutzeri DSM 10701

74.128

100

0.741
  pilT Legionella pneumophila strain Lp02

72.059

98.837

0.712
  pilT Legionella pneumophila strain ERS1305867

72.059

98.837

0.712
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.02

99.709

0.509
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398