Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PU634_RS01435 Genome accession   NZ_CP118224
Coordinates   298187..299296 (+) Length   369 a.a.
NCBI ID   WP_306762307.1    Uniprot ID   A0AA50KMV2
Organism   Oceanimonas pelagia strain NTOU-MSR1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 293187..304296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PU634_RS01400 (PU634_01400) rdgB 293440..294039 (-) 600 WP_306762300.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PU634_RS01405 (PU634_01405) - 294251..294664 (-) 414 WP_306762301.1 DUF4426 domain-containing protein -
  PU634_RS01410 (PU634_01410) - 294703..295014 (-) 312 WP_306762302.1 DUF167 family protein -
  PU634_RS01415 (PU634_01415) - 295002..295553 (-) 552 WP_306762303.1 YggT family protein -
  PU634_RS01420 (PU634_01420) proC 295563..296387 (-) 825 WP_306762304.1 pyrroline-5-carboxylate reductase -
  PU634_RS01425 (PU634_01425) - 296398..297090 (-) 693 WP_306762305.1 YggS family pyridoxal phosphate-dependent enzyme -
  PU634_RS01430 (PU634_01430) pilT 297132..298166 (+) 1035 WP_306762306.1 type IV pilus twitching motility protein PilT Machinery gene
  PU634_RS01435 (PU634_01435) pilU 298187..299296 (+) 1110 WP_306762307.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PU634_RS01440 (PU634_01440) yaaA 299304..300083 (+) 780 WP_306762308.1 peroxide stress protein YaaA -
  PU634_RS01445 (PU634_01445) - 300242..300391 (-) 150 WP_306762309.1 hypothetical protein -
  PU634_RS01450 (PU634_01450) srmB 300442..301740 (-) 1299 WP_306762310.1 ATP-dependent RNA helicase SrmB -
  PU634_RS01455 (PU634_01455) - 301811..302518 (+) 708 WP_306762311.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PU634_RS01460 (PU634_01460) brnQ 302713..303987 (+) 1275 WP_306763637.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41110.86 Da        Isoelectric Point: 5.9855

>NTDB_id=791098 PU634_RS01435 WP_306762307.1 298187..299296(+) (pilU) [Oceanimonas pelagia strain NTOU-MSR1]
MELASLLGRMCESKASDMYISVGVAPTIKSQGKMLPIGDHPLSEGEALALVESCMTDECRERFHREKEANFAIHDDEHGR
FRVSAFWQQDKAGMVLRRIESRIPTFEELYLPEVLREVAMAKRGLVLFVGGTGTGKSTTQAAMIGHRNRHAGGHILTIED
PVEFVHQHDQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSEETMGYALSFAETGHLCMATLHANNANQAIDRI
MHLVPENKHRQLLFDLSFNLKAIVAQQLIPTRDGNQRRGAFEILLNTPIVADVIRQGDMHRLKEIMGKSREAGMQTFDQA
LFDLYTRQHIGYTEALAYADSPNDLRLMIKLQGGGGLDSGLFDNVTIES

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=791098 PU634_RS01435 WP_306762307.1 298187..299296(+) (pilU) [Oceanimonas pelagia strain NTOU-MSR1]
ATGGAGCTGGCTTCATTACTGGGCAGAATGTGCGAGAGTAAGGCCTCGGACATGTACATTTCGGTGGGGGTGGCGCCCAC
CATCAAGAGTCAGGGCAAAATGCTGCCCATCGGCGACCACCCGCTGAGCGAGGGCGAGGCGCTGGCGCTGGTGGAATCCT
GCATGACCGACGAGTGCCGGGAGCGCTTTCACCGGGAGAAAGAGGCCAACTTCGCCATTCACGACGATGAGCACGGCCGG
TTCCGGGTGAGCGCCTTCTGGCAGCAGGACAAGGCCGGCATGGTGCTCAGACGCATTGAGAGCCGCATTCCCACCTTTGA
AGAGCTGTACCTGCCCGAGGTGCTGCGCGAGGTGGCCATGGCCAAGCGTGGCCTGGTGCTGTTTGTGGGCGGCACCGGCA
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCCACCGCAACCGCCATGCCGGCGGTCATATCCTCACCATTGAGGAT
CCGGTGGAGTTCGTGCACCAGCACGATCAGTCGATCATTACCCAGCGGGAAGTGGGCATCGACACCGAGTCGTTCGATGC
GGCCCTGAAAAGCTCGCTGCGTCAGGCGCCGGACGTGATTCTGATTGGTGAAATCCGCTCCGAGGAAACCATGGGGTATG
CGCTGTCGTTCGCCGAAACCGGCCACCTGTGCATGGCTACCCTGCACGCCAACAATGCCAACCAGGCCATTGATCGCATC
ATGCACCTGGTGCCGGAAAACAAGCACCGTCAGCTGTTGTTTGATCTGTCGTTCAACCTCAAGGCCATTGTGGCCCAGCA
ACTTATTCCCACCCGGGACGGCAACCAGCGCCGGGGGGCCTTTGAAATATTGCTGAACACCCCCATCGTGGCCGACGTCA
TTCGCCAGGGCGACATGCACCGGCTCAAGGAGATCATGGGCAAGTCCCGCGAGGCGGGCATGCAAACCTTTGATCAGGCG
CTGTTTGACCTCTATACGCGCCAGCATATAGGCTACACCGAAGCCCTGGCCTACGCCGATTCCCCCAACGATCTGCGGCT
GATGATCAAGCTGCAGGGCGGCGGCGGGCTCGACTCCGGCCTGTTTGACAACGTAACCATCGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.152

99.729

0.58
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

58.357

95.664

0.558
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

93.496

0.398
  pilT Neisseria gonorrhoeae MS11

41.194

90.786

0.374
  pilT Pseudomonas aeruginosa PAK

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

41.358

87.805

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.673

88.618

0.36
  pilT Vibrio cholerae strain A1552

40.673

88.618

0.36
  pilT Acinetobacter baylyi ADP1

38.889

92.683

0.36
  pilT Legionella pneumophila strain Lp02

40.181

89.702

0.36
  pilT Legionella pneumophila strain ERS1305867

40.181

89.702

0.36