Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PotMrB4_RS01915 Genome accession   NZ_AP022642
Coordinates   382110..383144 (+) Length   344 a.a.
NCBI ID   WP_044403590.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain MrB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 377110..388144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS01890 (PtoMrB4_03760) - 378342..378638 (-) 297 WP_172432360.1 DUF167 domain-containing protein -
  PotMrB4_RS01895 (PtoMrB4_03770) - 378638..379231 (-) 594 WP_074967194.1 YggT family protein -
  PotMrB4_RS01900 (PtoMrB4_03780) - 379323..380510 (-) 1188 WP_172432361.1 hypothetical protein -
  PotMrB4_RS01905 (PtoMrB4_03790) proC 380513..381331 (-) 819 WP_172432362.1 pyrroline-5-carboxylate reductase -
  PotMrB4_RS01910 (PtoMrB4_03800) - 381349..382041 (-) 693 WP_160480527.1 YggS family pyridoxal phosphate-dependent enzyme -
  PotMrB4_RS01915 (PtoMrB4_03810) pilT 382110..383144 (+) 1035 WP_044403590.1 type IV pilus twitching motility protein PilT Machinery gene
  PotMrB4_RS01920 (PtoMrB4_03820) pilU 383253..384398 (+) 1146 WP_044403589.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PotMrB4_RS01925 (PtoMrB4_03830) - 384340..385785 (-) 1446 WP_172432363.1 TIGR03915 family putative DNA repair protein -
  PotMrB4_RS01930 (PtoMrB4_03840) - 385785..386996 (-) 1212 WP_069566424.1 putative DNA modification/repair radical SAM protein -
  PotMrB4_RS01935 (PtoMrB4_03850) - 387103..387498 (+) 396 WP_142012809.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37835.60 Da        Isoelectric Point: 6.8360

>NTDB_id=79051 PotMrB4_RS01915 WP_044403590.1 382110..383144(+) (pilT) [Metapseudomonas otitidis strain MrB4]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNGTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDSCL
KGLVAKGLVSRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=79051 PotMrB4_RS01915 WP_044403590.1 382110..383144(+) (pilT) [Metapseudomonas otitidis strain MrB4]
ATGGATATTACTGAGCTGCTTGCGTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCGGCCGGGCTGCCGCCCAT
GATCCGCGTCGACGGTGATGTTCGCCGTATCAACCTGCCCCCCCTGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAAACCGACTTCTCGTTCGAAGTGCCCGGCGTGGCCCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGTCCATGGA
GGACCTGGGCATGGGCGAGATCTTCAAGAAGATCTCCGACGTGCCGCGGGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTCAACGGCACCAAGTACCACCACATCCTGACCGTGGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGATCAACCAGCGTGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTTGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCGATGGTGCGCTCCATGCTCTCCGAATCCCTGCAGGCGGTCATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCCGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGGCGCCCTGGGCATGCAGACCCTGGACAGCTGCCTG
AAGGGCCTGGTGGCCAAGGGCCTGGTCAGCCGCGAAAGCGCGCGCGAAAAGGCCAAGACGCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

94.767

100

0.948
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

86.047

100

0.86
  pilT Acinetobacter baumannii strain A118

86.047

100

0.86
  pilT Acinetobacter nosocomialis M2

85.756

100

0.858
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39

Multiple sequence alignment