Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PRJ39_RS20335 Genome accession   NZ_CP117873
Coordinates   4779737..4780774 (-) Length   345 a.a.
NCBI ID   WP_091798676.1    Uniprot ID   A0A1H3M5G3
Organism   Lysobacter enzymogenes strain Lj46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4774737..4785774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRJ39_RS20330 (PRJ39_19590) pilU 4778456..4779622 (-) 1167 WP_091798673.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PRJ39_RS20335 (PRJ39_19595) pilT 4779737..4780774 (-) 1038 WP_091798676.1 type IV pilus twitching motility protein PilT Machinery gene
  PRJ39_RS20340 (PRJ39_19600) - 4780953..4781645 (+) 693 WP_394538102.1 YggS family pyridoxal phosphate-dependent enzyme -
  PRJ39_RS20345 (PRJ39_19605) - 4781666..4782295 (-) 630 WP_394538103.1 HAD family hydrolase -
  PRJ39_RS20350 (PRJ39_19610) proC 4782406..4783242 (+) 837 WP_091798684.1 pyrroline-5-carboxylate reductase -
  PRJ39_RS20355 (PRJ39_19615) - 4783239..4783700 (+) 462 WP_394538104.1 DUF4426 domain-containing protein -
  PRJ39_RS20360 (PRJ39_19620) - 4783927..4784949 (-) 1023 WP_394538105.1 lipase secretion chaperone -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38516.34 Da        Isoelectric Point: 6.7271

>NTDB_id=788161 PRJ39_RS20335 WP_091798676.1 4779737..4780774(-) (pilT) [Lysobacter enzymogenes strain Lj46]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQSYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=788161 PRJ39_RS20335 WP_091798676.1 4779737..4780774(-) (pilT) [Lysobacter enzymogenes strain Lj46]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTAAAGAACAAAGCATCCGACTTGCATCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAGTTCCTGGAAGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGAGCCTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTCATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTCTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTCCACCGCGACACGCACGGGTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACCGTGGACCGCATC
ATCGACGTGTTTCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAATCGTTGCGCGCGGTGATCTCGCAAGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCATCCCGGCGATCCGTAACCTCATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGTCCTACGGCATGATGACCCTGGACCAGCACCTC
CAGGACCTGGTCAAGCGCGGCCTGATCCTGCGCCCGCAGGCCAAGGAATACGCCAAGGACAAGCGCCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H3M5G3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.652

0.391
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38