Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PRJ39_RS20330 Genome accession   NZ_CP117873
Coordinates   4778456..4779622 (-) Length   388 a.a.
NCBI ID   WP_091798673.1    Uniprot ID   A0A1H3M5M5
Organism   Lysobacter enzymogenes strain Lj46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4773456..4784622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRJ39_RS20330 (PRJ39_19590) pilU 4778456..4779622 (-) 1167 WP_091798673.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PRJ39_RS20335 (PRJ39_19595) pilT 4779737..4780774 (-) 1038 WP_091798676.1 type IV pilus twitching motility protein PilT Machinery gene
  PRJ39_RS20340 (PRJ39_19600) - 4780953..4781645 (+) 693 WP_394538102.1 YggS family pyridoxal phosphate-dependent enzyme -
  PRJ39_RS20345 (PRJ39_19605) - 4781666..4782295 (-) 630 WP_394538103.1 HAD family hydrolase -
  PRJ39_RS20350 (PRJ39_19610) proC 4782406..4783242 (+) 837 WP_091798684.1 pyrroline-5-carboxylate reductase -
  PRJ39_RS20355 (PRJ39_19615) - 4783239..4783700 (+) 462 WP_394538104.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43079.43 Da        Isoelectric Point: 6.8285

>NTDB_id=788160 PRJ39_RS20330 WP_091798673.1 4778456..4779622(-) (pilU) [Lysobacter enzymogenes strain Lj46]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKITPITQNPLTPQQSRDLVLNVMSPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=788160 PRJ39_RS20330 WP_091798673.1 4778456..4779622(-) (pilU) [Lysobacter enzymogenes strain Lj46]
ATGAACACGCCCGCCCCGACCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCACCCCGATCACCC
AGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCGTCCTCAACGTGATGAGCCCGCAGCAGCGCGAGGAGTTCGAGAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCCAGCGTCGACGAACTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGACGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCT
GTTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGGCGCG
TGGCGATGGAGATCCTGCTGGGCACCCCGCTGGCCCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAACTG
ATGAAGGAGTCGACCCAGCTCGGCATGAAGACCTTCGACCAGGCCTTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTA
CGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGACGCGCGGA
CCCTGTCGCAGGGGCTGGACGGGGTGGAGGTTGCGGAGGTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H3M5M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.937

94.588

0.652
  pilU Acinetobacter baylyi ADP1

65.266

92.01

0.601
  pilU Vibrio cholerae strain A1552

52.809

91.753

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.88

90.464

0.379