Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUO95_RS08325 Genome accession   NZ_CP117823
Coordinates   1865990..1867024 (+) Length   344 a.a.
NCBI ID   WP_004084948.1    Uniprot ID   A0A060GZY3
Organism   Xylella fastidiosa subsp. fastidiosa strain ICMP 8742     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1860990..1872024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO95_RS08305 (PUO95_08305) - 1861701..1862480 (+) 780 WP_004087296.1 beta-ketoacyl synthase chain length factor -
  PUO95_RS08310 (PUO95_08310) - 1862477..1863301 (+) 825 WP_011097969.1 glycosyltransferase family 2 protein -
  PUO95_RS08315 (PUO95_08315) - 1863935..1864762 (-) 828 WP_012382641.1 glycosyltransferase family 2 protein -
  PUO95_RS08320 (PUO95_08320) - 1865120..1865332 (-) 213 WP_004087301.1 hypothetical protein -
  PUO95_RS08325 (PUO95_08325) pilT 1865990..1867024 (+) 1035 WP_004084948.1 type IV pilus twitching motility protein PilT Machinery gene
  PUO95_RS08330 (PUO95_08330) pilU 1867190..1868320 (+) 1131 WP_011097971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUO95_RS08335 (PUO95_08335) - 1869013..1869981 (-) 969 WP_004087304.1 YafY family protein -
  PUO95_RS08340 (PUO95_08340) - 1870830..1871639 (+) 810 WP_004087308.1 DUF481 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38497.20 Da        Isoelectric Point: 6.8843

>NTDB_id=787787 PUO95_RS08325 WP_004084948.1 1865990..1867024(+) (pilT) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQRRDYEEALETDFSFEIPSLSR
FRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIEEPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIED
PIEFVHTSQKCLINQREVHRDTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRNLITRQQAREYAKDKANF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=787787 PUO95_RS08325 WP_004084948.1 1865990..1867024(+) (pilT) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
ATGGATATTGCTGAACTGTTAGCGTTTTCAGTCAAAAACAATGCTTCGGACCTGCACCTGTCAGCAGGCCTGCCACCAAT
GATCAGGGTGGACGGCGATGTACGCCGTATCAATATCCCCGCATTCGACCATAAGCAGGTGCAATCGCTAATTTACGACA
TCATGTCGGATAAGCAACGACGCGACTATGAGGAAGCCCTGGAGACGGATTTTTCGTTCGAAATCCCTTCACTCTCCCGC
TTCCGTGTCAATGCATTCAACCAAGAGCGTGGTGCTGGTGCCGTGTTTCGCACCATTCCCAATAAGGTGATGACATTGGA
CGAACTCGGCTGTTTGCCGGTGTTCCGCAAACTCATTGAAGAACCGCAGGGGTTGATTCTGGTGACTGGGCCGACTGGCT
CTGGAAAATCGACCACCCTCGCAGCCATGATCGACCATATCAACAAAAATGCTCACGGGCATATTCTCACGATAGAGGAC
CCGATTGAGTTCGTTCACACCTCACAGAAGTGCTTGATTAACCAACGTGAGGTACATCGCGATACTCACGACTTCAACAA
AGCACTCAGCTCAGCACTGCGCGAAGATCCAGACATCATCTTGATTGGAGAATTGCGTGACCTAGAAACGATCCGTCTAG
CACTGACTGCAGCCGAAACTGGCCACTTGGTATTTGGCACCCTGCACACCAATTCAGCAGCTAAAAGTATTAACCGCATC
ATCGACGTGTTTCCCGCTGCTGAAAAACCGATGGTCCGCTCTATGCTTTCCGAATCGCTCTGCGGGATCATCTCGCAAAT
GTTGTTGAAAAAAGTTGGTGGTGGACGTACCGCAGCCTGGGAAATTATGGTTGGCACCCCCGCAATCCGAAATTTGATCC
GTGAGGATAAAGTCGCACAGATGTATTCATCCATCCAAACTGGCCAGCAATATGGCATGCAGACCCTTGACCAGCATTTA
CAGGATCTCATCAAACGCAACCTGATCACACGACAGCAAGCACGTGAATACGCCAAGGATAAAGCGAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A060GZY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Pseudomonas aeruginosa PAK

74.709

100

0.747
  pilT Acinetobacter baumannii D1279779

74.419

100

0.744
  pilT Acinetobacter baylyi ADP1

74.419

100

0.744
  pilT Acinetobacter baumannii strain A118

74.419

100

0.744
  pilT Acinetobacter nosocomialis M2

74.419

100

0.744
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.06

97.384

0.663
  pilT Vibrio cholerae strain A1552

68.06

97.384

0.663
  pilT Neisseria gonorrhoeae MS11

62.463

99.128

0.619
  pilT Neisseria meningitidis 8013

62.17

99.128

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Pseudomonas stutzeri DSM 10701

40.698

100

0.407
  pilU Vibrio cholerae strain A1552

39.826

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398