Detailed information NTG0787788

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PUO95_RS08330
• Genome accession: NZ_CP117823
• Coordinates: 1867190..1868320 (+)
• Length: 376 a.a.
• NCBI ID: WP_011097971.1
• Uniprot ID: Q87CD3
• Organism: Xylella fastidiosa subsp. fastidiosa strain ICMP 8742
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1862190..1873320

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUO95_RS08310 (PUO95_08310)	-	1862477..1863301 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
PUO95_RS08315 (PUO95_08315)	-	1863935..1864762 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
PUO95_RS08320 (PUO95_08320)	-	1865120..1865332 (-)	213	WP_004087301.1	hypothetical protein	-
PUO95_RS08325 (PUO95_08325)	pilT	1865990..1867024 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
PUO95_RS08330 (PUO95_08330)	pilU	1867190..1868320 (+)	1131	WP_011097971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PUO95_RS08335 (PUO95_08335)	-	1869013..1869981 (-)	969	WP_004087304.1	YafY family protein	-
PUO95_RS08340 (PUO95_08340)	-	1870830..1871639 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
PUO95_RS08345 (PUO95_08345)	hemC	1871693..1872610 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42479.73 Da
• Isoelectric Point: 6.5563

>NTDB_id=787788 PUO95_RS08330 WP_011097971.1 1867190..1868320(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=787788 PUO95_RS08330 WP_011097971.1 1867190..1868320(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: Q87CD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402