NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	PT256_RS09290	Genome accession	NZ_CP117817
Coordinates	2028665..2029078 (-)	Length	137 a.a.
NCBI ID	WP_042754743.1	Uniprot ID	-
Organism	Legionella pneumophila strain D5573
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2023665..2034078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PT256_RS09275 (PT256_09275)	letS	2023979..2026711 (+)	2733	WP_011946809.1	two-component system sensor histidine kinase LetS	Regulator
PT256_RS09280 (PT256_09280)	-	2026809..2028053 (+)	1245	WP_011946810.1	6-phosphofructokinase	-
PT256_RS09285 (PT256_09285)	pilA2	2028050..2028463 (-)	414	WP_011946811.1	pilin	Machinery gene
PT256_RS09290 (PT256_09290)	pilA2	2028665..2029078 (-)	414	WP_042754743.1	pilin	Machinery gene
PT256_RS09295 (PT256_09295)	-	2029381..2029698 (+)	318	WP_042754744.1	BolA/IbaG family iron-sulfur metabolism protein	-
PT256_RS09300 (PT256_09300)	-	2029773..2031194 (+)	1422	WP_042754745.1	APC family permease	-
PT256_RS09305 (PT256_09305)	-	2031325..2032755 (-)	1431	WP_042754746.1	cellulase family glycosylhydrolase	-
PT256_RS09310 (PT256_09310)	kdsB	2033002..2033754 (-)	753	WP_027220383.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
PT256_RS09315 (PT256_09315)	-	2033751..2033930 (-)	180	WP_010947637.1	Trm112 family protein	-

Sequence

Protein

Download Length: 137 a.a. Molecular weight: 14023.23 Da Isoelectric Point: 8.4715

                                >NTDB_id=787699 PT256_RS09290 WP_042754743.1 2028665..2029078(-) (pilA2) [Legionella pneumophila strain D5573]

MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVSEGLQMASSAKLAVSETAITNNALPPTQAATGYVSPAPTANV

TGIAIGANGVITITYTAAAGGGTIIMTPTLDANNGDVTWNCTGGSLLPKYRPASCRP

Nucleotide

Download Length: 414 bp

                                >NTDB_id=787699 PT256_RS09290 WP_042754743.1 2028665..2029078(-) (pilA2) [Legionella pneumophila strain D5573]

ATGAGACAAAAGGGTTTTACATTAATTGAATTGATGATAGTCGTAGCGATTATCGGTATTTTGGCAGCAATCGCTATACC

AGCCTATCAAGATTACACCATACGAGCTCGTGTAAGCGAGGGGCTACAGATGGCAAGCTCAGCCAAACTTGCCGTATCTG

AAACGGCTATTACAAACAATGCGTTACCACCTACTCAGGCTGCAACGGGCTACGTGTCTCCTGCCCCCACGGCTAATGTG

ACAGGTATTGCAATAGGTGCTAACGGCGTGATTACCATCACGTATACCGCTGCAGCAGGTGGTGGTACGATTATTATGAC

TCCTACACTGGATGCCAACAACGGTGATGTTACCTGGAACTGCACCGGTGGTAGCTTATTGCCTAAATACCGGCCTGCCA

GTTGCAGACCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila strain ERS1305867	89.781	100	0.898
pilA2	Legionella pneumophila str. Paris	89.051	100	0.891
pilA	Ralstonia pseudosolanacearum GMI1000	47.826	100	0.562
comP	Acinetobacter baylyi ADP1	52.055	100	0.555
pilE	Neisseria gonorrhoeae MS11	39.241	100	0.453
pilA	Pseudomonas aeruginosa PAK	40.94	100	0.445
pilA	Haemophilus influenzae 86-028NP	41.096	100	0.438
pilE	Neisseria gonorrhoeae strain FA1090	37.58	100	0.431
pilA/pilA1	Eikenella corrodens VA1	38.312	100	0.431
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.176	99.27	0.409
pilA	Glaesserella parasuis strain SC1401	40.441	99.27	0.401
pilA/pilAII	Pseudomonas stutzeri DSM 10701	38.849	100	0.394
pilA	Vibrio parahaemolyticus RIMD 2210633	40.8	91.241	0.372
pilA	Vibrio cholerae C6706	37.226	100	0.372
pilA	Vibrio cholerae strain A1552	37.226	100	0.372
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.226	100	0.372

Detailed information NTG0787699