Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   PT256_RS09285 Genome accession   NZ_CP117817
Coordinates   2028050..2028463 (-) Length   137 a.a.
NCBI ID   WP_011946811.1    Uniprot ID   A0AAN5R315
Organism   Legionella pneumophila strain D5573     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2023050..2033463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT256_RS09275 (PT256_09275) letS 2023979..2026711 (+) 2733 WP_011946809.1 two-component system sensor histidine kinase LetS Regulator
  PT256_RS09280 (PT256_09280) - 2026809..2028053 (+) 1245 WP_011946810.1 6-phosphofructokinase -
  PT256_RS09285 (PT256_09285) pilA2 2028050..2028463 (-) 414 WP_011946811.1 pilin Machinery gene
  PT256_RS09290 (PT256_09290) pilA2 2028665..2029078 (-) 414 WP_042754743.1 pilin Machinery gene
  PT256_RS09295 (PT256_09295) - 2029381..2029698 (+) 318 WP_042754744.1 BolA/IbaG family iron-sulfur metabolism protein -
  PT256_RS09300 (PT256_09300) - 2029773..2031194 (+) 1422 WP_042754745.1 APC family permease -
  PT256_RS09305 (PT256_09305) - 2031325..2032755 (-) 1431 WP_042754746.1 cellulase family glycosylhydrolase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14681.10 Da        Isoelectric Point: 7.7736

>NTDB_id=787698 PT256_RS09285 WP_011946811.1 2028050..2028463(-) (pilA2) [Legionella pneumophila strain D5573]
MRTKGFTLIELMIVAAILGILLAIAFPAYQDYTIRARVAEGLEKASFAKLAVAEKILIDNILPLNQADTSYSSPIPTDSV
ASIAIGNQGIITITYTANAGNGTITMVPTIDATNRILTWDCTGGTLISKYRPAYCRP

Nucleotide


Download         Length: 414 bp        

>NTDB_id=787698 PT256_RS09285 WP_011946811.1 2028050..2028463(-) (pilA2) [Legionella pneumophila strain D5573]
ATGAGAACGAAAGGATTTACTTTAATCGAGCTGATGATCGTTGCTGCTATCCTTGGGATTCTACTTGCCATCGCTTTTCC
AGCCTATCAGGACTATACCATCAGAGCGCGGGTGGCTGAAGGATTGGAAAAGGCTTCATTTGCAAAATTAGCCGTTGCCG
AGAAGATTTTGATAGATAATATATTGCCATTAAATCAAGCAGATACTAGTTACTCAAGTCCCATACCCACAGACAGTGTC
GCCTCTATAGCTATTGGCAATCAAGGCATAATTACCATTACCTACACGGCAAATGCAGGTAATGGCACTATTACAATGGT
TCCTACAATAGATGCCACTAACAGAATACTCACTTGGGATTGCACTGGTGGTACCCTGATTAGTAAATATCGTCCAGCCT
ACTGCCGCCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-26)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

68.613

100

0.686
  pilA2 Legionella pneumophila strain ERS1305867

67.883

100

0.679
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

100

0.526
  comP Acinetobacter baylyi ADP1

45.517

100

0.482
  pilA/pilA1 Eikenella corrodens VA1

38.065

100

0.431
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.406

100

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.957

100

0.372