Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PGW97_RS13990 Genome accession   NZ_CP117517
Coordinates   3090955..3091992 (-) Length   345 a.a.
NCBI ID   WP_217394146.1    Uniprot ID   -
Organism   Photobacterium sp. GSS17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3085955..3096992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW97_RS13965 rsmE 3086485..3087216 (+) 732 WP_047887439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PGW97_RS13970 gshB 3087247..3088197 (+) 951 WP_207103469.1 glutathione synthase -
  PGW97_RS13975 - 3088328..3088894 (+) 567 WP_047887441.1 YqgE/AlgH family protein -
  PGW97_RS13980 ruvX 3088891..3089319 (+) 429 WP_217394142.1 Holliday junction resolvase RuvX -
  PGW97_RS13985 pilU 3089824..3090930 (-) 1107 WP_047887443.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PGW97_RS13990 pilT 3090955..3091992 (-) 1038 WP_217394146.1 type IV pilus twitching motility protein PilT Machinery gene
  PGW97_RS13995 - 3092016..3092732 (+) 717 WP_273857527.1 YggS family pyridoxal phosphate-dependent enzyme -
  PGW97_RS14000 proC 3092985..3093803 (+) 819 WP_273857528.1 pyrroline-5-carboxylate reductase -
  PGW97_RS14005 - 3093817..3094371 (+) 555 WP_047887447.1 YggT family protein -
  PGW97_RS14010 yggU 3094368..3094661 (+) 294 WP_273857529.1 DUF167 family protein YggU -
  PGW97_RS14015 - 3094795..3095232 (+) 438 WP_047887449.1 DUF4426 domain-containing protein -
  PGW97_RS14020 - 3095325..3095918 (+) 594 WP_217394154.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38380.77 Da        Isoelectric Point: 6.3386

>NTDB_id=786186 PGW97_RS13990 WP_217394146.1 3090955..3091992(-) (pilT) [Photobacterium sp. GSS17]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPSLEHSEVHRLVFDIMNDAQRREFEEKLEVDFSFELPDVGR
FRVNAFHQSRGCAAVFRTIPVHVPTLESLKVPDIFYQIAQCQRGLVLVTGATGSGKSTTIAALVDYINENYNRHILTIED
PVEFVHHGKRCLINQREVHRDTHSFHNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGNDKAMVRSMLSESLRAVVSQSLLKCTSGGRIAAHEVMMATPAIRNLIREDKVAQMYSMIQTGSSVGMQTMEQSV
RMLVAQGQVEAEEGRRVVDNAFAQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=786186 PGW97_RS13990 WP_217394146.1 3090955..3091992(-) (pilT) [Photobacterium sp. GSS17]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCGGATCTGCACCTCTCTGCGGGAGTTCCGCCGAT
GATTCGGGTAGATGGCGATGTTCGTAAACTGAGCTTGCCATCACTGGAGCACAGTGAAGTACACCGCTTGGTGTTTGACA
TTATGAACGATGCCCAGCGGCGCGAGTTTGAAGAAAAGCTCGAAGTCGATTTCTCGTTCGAGCTGCCGGACGTCGGGCGG
TTTCGTGTGAATGCCTTTCATCAGTCACGGGGATGCGCAGCGGTTTTCCGAACCATCCCGGTGCATGTGCCGACACTGGA
ATCACTGAAAGTGCCGGACATATTCTATCAGATCGCCCAATGCCAGCGCGGGCTGGTGCTGGTTACCGGGGCAACCGGCT
CCGGTAAATCCACCACGATTGCTGCGCTGGTCGATTACATCAATGAAAACTATAACCGCCATATCCTGACGATTGAAGAT
CCGGTGGAGTTTGTTCACCACGGCAAACGTTGCCTGATTAACCAGCGTGAAGTACACCGCGATACCCACAGTTTCCACAA
TGCACTGCGATCTTCCCTGCGTGAAGACCCGGATGTGATTGTGGTGGGGGAATTGCGCGATCAGGAAACCATCAGTCTGG
CGCTGACCGCGGCAGAAACCGGCCACCTTGTGCTGGGCACTCTGCACACCAGCTCGGCGGCCAAAACCGTCGATCGGATC
ATTGATGTCTTCCCGGGGAACGACAAGGCGATGGTGCGTTCTATGTTGTCTGAGTCGCTGCGGGCGGTGGTATCGCAGAG
CCTGCTTAAATGTACCAGCGGAGGCCGAATTGCGGCTCATGAAGTCATGATGGCGACCCCGGCTATCCGTAACCTGATCC
GCGAAGACAAAGTGGCCCAGATGTATTCCATGATCCAGACCGGCTCATCCGTCGGGATGCAGACCATGGAGCAAAGTGTC
CGGATGCTGGTGGCTCAGGGGCAGGTTGAGGCGGAAGAAGGACGCCGGGTCGTCGACAACGCGTTTGCGCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.477

96.522

0.748
  pilT Vibrio cholerae strain A1552

77.477

96.522

0.748
  pilT Acinetobacter baumannii D1279779

70.448

97.101

0.684
  pilT Acinetobacter baumannii strain A118

70.448

97.101

0.684
  pilT Acinetobacter nosocomialis M2

70.149

97.101

0.681
  pilT Acinetobacter baylyi ADP1

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

68.657

97.101

0.667
  pilT Pseudomonas stutzeri DSM 10701

67.463

97.101

0.655
  pilT Legionella pneumophila strain Lp02

67.89

94.783

0.643
  pilT Legionella pneumophila strain ERS1305867

67.89

94.783

0.643
  pilT Neisseria meningitidis 8013

64.035

99.13

0.635
  pilT Neisseria gonorrhoeae MS11

63.743

99.13

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.974

98.841

0.484
  pilU Vibrio cholerae strain A1552

41.228

99.13

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

39.13

93.333

0.365