Detailed information NTG0786185

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PGW97_RS13985
• Genome accession: NZ_CP117517
• Coordinates: 3089824..3090930 (-)
• Length: 368 a.a.
• NCBI ID: WP_047887443.1
• Uniprot ID: A0A0J1GZT4
• Organism: Photobacterium sp. GSS17
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3084824..3095930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PGW97_RS13960	endA	3085536..3086240 (+)	705	WP_217394135.1	deoxyribonuclease I	-
PGW97_RS13965	rsmE	3086485..3087216 (+)	732	WP_047887439.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
PGW97_RS13970	gshB	3087247..3088197 (+)	951	WP_207103469.1	glutathione synthase	-
PGW97_RS13975	-	3088328..3088894 (+)	567	WP_047887441.1	YqgE/AlgH family protein	-
PGW97_RS13980	ruvX	3088891..3089319 (+)	429	WP_217394142.1	Holliday junction resolvase RuvX	-
PGW97_RS13985	pilU	3089824..3090930 (-)	1107	WP_047887443.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PGW97_RS13990	pilT	3090955..3091992 (-)	1038	WP_217394146.1	type IV pilus twitching motility protein PilT	Machinery gene
PGW97_RS13995	-	3092016..3092732 (+)	717	WP_273857527.1	YggS family pyridoxal phosphate-dependent enzyme	-
PGW97_RS14000	proC	3092985..3093803 (+)	819	WP_273857528.1	pyrroline-5-carboxylate reductase	-
PGW97_RS14005	-	3093817..3094371 (+)	555	WP_047887447.1	YggT family protein	-
PGW97_RS14010	yggU	3094368..3094661 (+)	294	WP_273857529.1	DUF167 family protein YggU	-
PGW97_RS14015	-	3094795..3095232 (+)	438	WP_047887449.1	DUF4426 domain-containing protein	-
PGW97_RS14020	-	3095325..3095918 (+)	594	WP_217394154.1	XTP/dITP diphosphatase	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 41183.25 Da
• Isoelectric Point: 6.1452

>NTDB_id=786185 PGW97_RS13985 WP_047887443.1 3089824..3090930(-) (pilU) [Photobacterium sp. GSS17]
MTLQDALNEMVSSKASDLYITVEAPCLLKIDGNLHPLGQALDRPAIDRMFSEAMDDALYQEFCATKEANFALVRGEYRFR
VSAFWQRELPGMVIRRIETDIPDIFALNLPMDMQKMALAKRGLVLVVGATGSGKSTSLAAMTGYRNANRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDAHGMGRHGAFELLINTPRVADLIRRGELHEIKDTMAKSAQSGMMTFDQSLY
DLYSAGKITEQDALHHADSANDLRLMIKVGNKHHQANSSLDGVTIDMD

Nucleotide

• Length: 1107 bp

>NTDB_id=786185 PGW97_RS13985 WP_047887443.1 3089824..3090930(-) (pilU) [Photobacterium sp. GSS17]
ATGACATTACAGGATGCGCTGAATGAAATGGTCAGCAGCAAAGCCTCCGATTTGTACATCACGGTGGAAGCGCCATGTCT
GCTGAAAATCGACGGTAACCTGCACCCGCTGGGGCAGGCGCTGGATCGCCCGGCGATTGACCGGATGTTCAGCGAAGCCA
TGGACGATGCGTTGTATCAGGAGTTTTGTGCCACTAAAGAAGCAAACTTTGCGCTGGTACGCGGCGAGTATCGGTTCCGG
GTCAGTGCCTTCTGGCAGCGGGAACTGCCGGGCATGGTGATCCGGCGGATTGAAACCGATATCCCTGACATTTTTGCCCT
TAACTTGCCGATGGACATGCAGAAGATGGCACTGGCGAAACGGGGGCTGGTGCTGGTGGTCGGGGCGACCGGCTCGGGCA
AATCTACGTCACTGGCGGCGATGACCGGCTACCGTAATGCCAACCGTAGCGGTCACATTCTGACGGTGGAAGATCCGATT
GAGTTCGTGCACAAGCACAATAAGTGCATCGTGACCCAGCGCGAAGTCGGCCTGGATACGGAGAGCTACGAGGTGGCGCT
GAAGAACTCGCTGCGCCAGGCTCCTGACATGATTCTGATTGGCGAAATCCGGACCCGGGAAACCATGGAATATGCCATGA
ACTTTGCCGAAACCGGGCACCTGTGTATGGCGACCTTGCATGCCAATAATGCCAACCAGGCGTTGGAGCGTATTCTCCAT
CTGGTTCCGAAAGAGCGGCGGGATCAGTTTCTGTTCGATTTATCACTGAACCTGAAATGTATTCTGGCTCAGCAGCTGAT
CCCGGATGCCCACGGTATGGGACGGCACGGTGCCTTTGAGCTGTTGATCAACACGCCACGGGTTGCGGATCTGATCCGTC
GGGGTGAGCTGCACGAAATCAAGGACACCATGGCGAAATCAGCCCAGTCGGGGATGATGACGTTTGATCAGTCTCTGTAT
GACCTCTATAGCGCCGGCAAGATCACGGAGCAGGACGCTTTGCACCATGCCGATTCGGCCAACGATCTGCGACTGATGAT
TAAAGTCGGCAACAAACATCATCAGGCAAACAGCAGCTTGGATGGGGTCACGATCGATATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (109-268)

3D structure

• AlphaFold DB: A0A0J1GZT4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	69.837	100	0.698
pilU	Pseudomonas stutzeri DSM 10701	58.221	100	0.587
pilU	Acinetobacter baylyi ADP1	54.795	99.185	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.653	91.576	0.372