Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LYZ37_RS01725 Genome accession   NZ_CP117029
Coordinates   353050..354087 (+) Length   345 a.a.
NCBI ID   WP_004742512.1    Uniprot ID   F9SZF7
Organism   Vibrio tubiashii strain FP17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348050..359087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYZ37_RS01690 (LYZ37_01690) hemW 348258..349433 (-) 1176 WP_272786212.1 radical SAM family heme chaperone HemW -
  LYZ37_RS01695 (LYZ37_01695) - 349452..350057 (-) 606 WP_272786213.1 XTP/dITP diphosphatase -
  LYZ37_RS01700 (LYZ37_01700) - 350092..350523 (-) 432 WP_004742507.1 DUF4426 domain-containing protein -
  LYZ37_RS01705 (LYZ37_01705) yggU 350577..350867 (-) 291 WP_004742508.1 DUF167 family protein YggU -
  LYZ37_RS01710 (LYZ37_01710) - 350867..351424 (-) 558 WP_272786214.1 YggT family protein -
  LYZ37_RS01715 (LYZ37_01715) proC 351464..352282 (-) 819 WP_272786215.1 pyrroline-5-carboxylate reductase -
  LYZ37_RS01720 (LYZ37_01720) - 352308..353024 (-) 717 WP_239826815.1 YggS family pyridoxal phosphate-dependent enzyme -
  LYZ37_RS01725 (LYZ37_01725) pilT 353050..354087 (+) 1038 WP_004742512.1 type IV pilus twitching motility protein PilT Machinery gene
  LYZ37_RS01730 (LYZ37_01730) pilU 354098..355204 (+) 1107 WP_239826814.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYZ37_RS01735 (LYZ37_01735) - 355292..355447 (-) 156 WP_004742514.1 FeoB-associated Cys-rich membrane protein -
  LYZ37_RS01740 (LYZ37_01740) feoB 355450..357951 (-) 2502 WP_272786216.1 ferrous iron transport protein B -
  LYZ37_RS01745 (LYZ37_01745) - 357975..358205 (-) 231 WP_272786217.1 FeoA family protein -
  LYZ37_RS01750 (LYZ37_01750) - 358425..358736 (+) 312 WP_069667657.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38184.60 Da        Isoelectric Point: 6.1455

>NTDB_id=782095 LYZ37_RS01725 WP_004742512.1 353050..354087(+) (pilT) [Vibrio tubiashii strain FP17]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFSHADVHRLVFEIMNDGQRSEFEERLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPTEIPTLEQLSAPQIFESISNMEKGLVLVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMFSVIQTGAAHGMQTMEQNA
KQLIAQGLVDQDEVAKKIELETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=782095 LYZ37_RS01725 WP_004742512.1 353050..354087(+) (pilT) [Vibrio tubiashii strain FP17]
ATGGATATCGCCGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCCGGAGTATCTCCAAT
GGTACGGATAGATGGTGAAGTAAGAAAGCTTGGCGTGCCTGCTTTTAGCCATGCAGATGTGCATCGTTTAGTATTCGAAA
TTATGAATGATGGTCAACGCAGTGAATTTGAAGAGCGCCTTGAAGTCGACTTTTCTTTTGAGTTGCCGAATGTTGGTCGC
TTCCGTGTTAATGCATTTAACCAAGCGCGTGGTTGCGCAGCGGTATTTCGTACCATTCCGACTGAGATTCCGACCTTAGA
GCAATTGTCAGCCCCACAAATCTTCGAAAGTATCTCTAACATGGAAAAGGGTTTGGTATTGGTGACAGGCCCTACAGGCT
CGGGTAAATCCACTACTTTGGCGGCGATGGTTGACCATATCAACCGCAACCACAATAAGCATATTCTGACGATTGAAGAC
CCAATTGAATTTGTTCATAGCAATAATAAGTGCTTAATCAACCAACGCGAAGTTCATCGAGATACGCATAGTTTTAATAA
TGCGCTGCGCAGTGCACTGCGTGAAGACCCAGACGTGATCTTAGTGGGTGAATTGCGTGACCAAGAGACAATCAGTCTAG
CACTGACAGCAGCAGAAACCGGGCACTTGGTGTTTGGGACTTTGCATACCAGTTCAGCGGCAAAAACCATCGACCGTATT
ATCGATGTTTTCCCGGGGAGTGATAAGGACATGGTTCGCTCTATGCTCTCTGAGTCATTACGCGCAGTCATTGCACAAAA
GTTGCTGAAGCGTATTGGCGGTGGTCGTACCGCTTGCCATGAGATCATGATGGCGACACCGGCGATCCGTAACTTGATCC
GTGAAGACAAGGTTGCTCAGATGTTTTCTGTGATTCAAACCGGTGCGGCACATGGCATGCAAACCATGGAGCAAAATGCC
AAGCAGTTGATTGCGCAAGGTCTTGTTGATCAGGATGAAGTAGCGAAGAAGATCGAACTTGAAACTTCAATGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9SZF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

72.34

95.362

0.69
  pilT Acinetobacter baumannii strain A118

72.34

95.362

0.69
  pilT Acinetobacter nosocomialis M2

72.036

95.362

0.687
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas stutzeri DSM 10701

67.953

97.681

0.664
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

64.793

97.971

0.635
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.497

96.812

0.499
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.159

95.072

0.391
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374