Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LYZ37_RS01730 Genome accession   NZ_CP117029
Coordinates   354098..355204 (+) Length   368 a.a.
NCBI ID   WP_239826814.1    Uniprot ID   -
Organism   Vibrio tubiashii strain FP17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349098..360204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYZ37_RS01695 (LYZ37_01695) - 349452..350057 (-) 606 WP_272786213.1 XTP/dITP diphosphatase -
  LYZ37_RS01700 (LYZ37_01700) - 350092..350523 (-) 432 WP_004742507.1 DUF4426 domain-containing protein -
  LYZ37_RS01705 (LYZ37_01705) yggU 350577..350867 (-) 291 WP_004742508.1 DUF167 family protein YggU -
  LYZ37_RS01710 (LYZ37_01710) - 350867..351424 (-) 558 WP_272786214.1 YggT family protein -
  LYZ37_RS01715 (LYZ37_01715) proC 351464..352282 (-) 819 WP_272786215.1 pyrroline-5-carboxylate reductase -
  LYZ37_RS01720 (LYZ37_01720) - 352308..353024 (-) 717 WP_239826815.1 YggS family pyridoxal phosphate-dependent enzyme -
  LYZ37_RS01725 (LYZ37_01725) pilT 353050..354087 (+) 1038 WP_004742512.1 type IV pilus twitching motility protein PilT Machinery gene
  LYZ37_RS01730 (LYZ37_01730) pilU 354098..355204 (+) 1107 WP_239826814.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYZ37_RS01735 (LYZ37_01735) - 355292..355447 (-) 156 WP_004742514.1 FeoB-associated Cys-rich membrane protein -
  LYZ37_RS01740 (LYZ37_01740) feoB 355450..357951 (-) 2502 WP_272786216.1 ferrous iron transport protein B -
  LYZ37_RS01745 (LYZ37_01745) - 357975..358205 (-) 231 WP_272786217.1 FeoA family protein -
  LYZ37_RS01750 (LYZ37_01750) - 358425..358736 (+) 312 WP_069667657.1 hypothetical protein -
  LYZ37_RS01755 (LYZ37_01755) - 358762..359547 (+) 786 WP_171326114.1 DUF4198 domain-containing protein -
  LYZ37_RS01760 (LYZ37_01760) ruvX 359620..360045 (-) 426 WP_045957217.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41363.13 Da        Isoelectric Point: 6.1935

>NTDB_id=782096 LYZ37_RS01730 WP_239826814.1 354098..355204(+) (pilU) [Vibrio tubiashii strain FP17]
MDIDVFLQQMIDQNASDLYITVDAPILYRVDGELRAHGDKLSHAVVSSLLDGIMDHDRQHEFHSSKEANFAIVRHFGRFR
VSAFYQRELPGAVIRRIETKIPTFDELKLPDVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGFRNSHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGELHELKATMAKSKEVGMQTFDQALY
QLVVEDKISEEDAMHSADSANDLRLMLKTQRGDVSSSGSLSGVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=782096 LYZ37_RS01730 WP_239826814.1 354098..355204(+) (pilU) [Vibrio tubiashii strain FP17]
ATGGATATTGATGTTTTTTTGCAACAGATGATCGACCAGAATGCGTCTGATCTTTACATCACAGTTGATGCGCCAATTCT
TTACCGTGTCGATGGAGAGCTGCGTGCACACGGAGATAAACTATCGCACGCTGTTGTGAGCTCTTTGTTGGATGGGATTA
TGGATCATGATCGCCAACACGAGTTTCATTCGAGCAAAGAAGCGAACTTTGCCATTGTGCGTCATTTTGGTCGATTCCGT
GTCTCTGCATTCTATCAGCGCGAATTGCCCGGGGCGGTCATTCGTCGAATAGAAACCAAGATTCCGACCTTTGATGAGCT
TAAGTTACCAGATGTTTTGCAAGACTTATCGATTGCGAAACGGGGTTTGGTGTTGGTGGTTGGAGCGACGGGTTCGGGTA
AGTCAACAACCATGGCTGCAATGACTGGGTTTCGCAATAGTCACCGGACAGGGCATATTTTGACGGTGGAAGACCCGATA
GAGTTTGTCCATGAGCATCAGCGTTGTATTGTGACACAGCGCGAGGTGGGGTTAGATACCGAAAGCTATGAAGTGGCATT
GAAGAACTCACTGCGCCAAGCTCCAGATATGATCTTAATAGGTGAGATTCGAACGCGAGAGACGATGGAGTACGCGATGA
CATTTGCTGAAACTGGGCATTTGTGTATGGCAACCCTTCATGCAAACAATGCCAACCAAGCGTTAGAGCGTATCCTCCAT
CTGGTGCCTAAAGAGCAAAAAGAGCAGTTTCTATTTGATTTATCGATGAACCTACGTGGTGTGATAGGTCAGCAACTTAT
TCGAGATAAAAATGGTCAAGGTCGGCACGGCGTGTTTGAAGTTTTACTCAACAGCCCACGCGTGTCGGATCTGATTCGTC
GTGGTGAGCTGCATGAGCTCAAAGCAACCATGGCTAAATCGAAAGAGGTCGGCATGCAAACCTTTGATCAGGCGCTTTAT
CAGCTAGTAGTGGAAGACAAGATCAGTGAAGAAGATGCCATGCACAGTGCGGATTCAGCCAATGATTTACGTCTAATGCT
TAAAACACAACGTGGTGATGTATCTAGCTCGGGTTCACTCAGTGGTGTGAAGATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.056

92.663

0.38
  pilT Legionella pneumophila strain ERS1305867

41.003

92.12

0.378
  pilT Legionella pneumophila strain Lp02

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.231

88.315

0.364
  pilT Vibrio cholerae strain A1552

41.231

88.315

0.364