Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL424_RS10990 Genome accession   NZ_CP117020
Coordinates   2200088..2201068 (-) Length   326 a.a.
NCBI ID   WP_085018120.1    Uniprot ID   -
Organism   Escherichia coli strain MLI106K3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195088..2206068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL424_RS10960 (NL424_010955) yggI 2195812..2196309 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  NL424_RS10965 (NL424_010960) endA 2196404..2197111 (+) 708 WP_000286497.1 deoxyribonuclease I -
  NL424_RS10970 (NL424_010965) rsmE 2197191..2197922 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NL424_RS10975 (NL424_010970) gshB 2197935..2198885 (+) 951 WP_000593273.1 glutathione synthase -
  NL424_RS10980 (NL424_010975) yqgE 2198994..2199557 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  NL424_RS10985 (NL424_010980) ruvX 2199557..2199973 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NL424_RS10990 (NL424_010985) pilT 2200088..2201068 (-) 981 WP_085018120.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NL424_RS10995 (NL424_010990) yggS 2201086..2201790 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NL424_RS11000 (NL424_010995) yggT 2201808..2202374 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NL424_RS11005 (NL424_011000) yggU 2202371..2202661 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  NL424_RS11010 (NL424_011005) rdgB 2202669..2203262 (+) 594 WP_001174746.1 XTP/dITP diphosphatase -
  NL424_RS11015 (NL424_011010) hemW 2203255..2204391 (+) 1137 WP_000239973.1 radical SAM family heme chaperone HemW -
  NL424_RS11020 (NL424_011015) - 2204706..2205692 (+) 987 WP_000784012.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35960.14 Da        Isoelectric Point: 5.7980

>NTDB_id=782045 NL424_RS10990 WP_085018120.1 2200088..2201068(-) (pilT) [Escherichia coli strain MLI106K3]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDASDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGVPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=782045 NL424_RS10990 WP_085018120.1 2200088..2201068(-) (pilT) [Escherichia coli strain MLI106K3]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGTCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCAGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGTACCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGTTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362