Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PKO50_RS28580 Genome accession   NZ_CP116775
Coordinates   6234265..6235299 (-) Length   344 a.a.
NCBI ID   WP_109932577.1    Uniprot ID   -
Organism   Pseudomonas sp. 273     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6229265..6240299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKO50_RS28560 - 6229641..6230813 (-) 1173 WP_275627906.1 cystathionine gamma-synthase -
  PKO50_RS28565 - 6230810..6232183 (-) 1374 WP_275627907.1 pyridoxal-phosphate dependent enzyme -
  PKO50_RS28570 - 6232472..6232876 (-) 405 WP_275627908.1 TM2 domain-containing protein -
  PKO50_RS28575 pilU 6233017..6234162 (-) 1146 WP_074977840.1 type IV pilus ATPase PilU Machinery gene
  PKO50_RS28580 pilT 6234265..6235299 (-) 1035 WP_109932577.1 type IV pilus twitching motility protein PilT Machinery gene
  PKO50_RS28585 - 6235373..6236062 (+) 690 WP_275627909.1 YggS family pyridoxal phosphate-dependent enzyme -
  PKO50_RS28590 proC 6236079..6236897 (+) 819 WP_275627910.1 pyrroline-5-carboxylate reductase -
  PKO50_RS28595 - 6236908..6237501 (+) 594 WP_089390951.1 YggT family protein -
  PKO50_RS28600 - 6237501..6237797 (+) 297 WP_089390952.1 DUF167 domain-containing protein -
  PKO50_RS28605 - 6237908..6239875 (+) 1968 WP_109932581.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37882.76 Da        Isoelectric Point: 6.7752

>NTDB_id=780235 PKO50_RS28580 WP_109932577.1 6234265..6235299(-) (pilT) [Pseudomonas sp. 273]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KSLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=780235 PKO50_RS28580 WP_109932577.1 6234265..6235299(-) (pilT) [Pseudomonas sp. 273]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGACCTGGGCATGGGCGAAGTGTTCAAGCGTGTTTCCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCTCGCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTG
AAGTCCCTGGTGGCCAAGGGCCTGGTGACCCGCGACGCCGCCAAGGAAAAGGCCAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.64

100

0.956
  pilT Pseudomonas stutzeri DSM 10701

93.023

100

0.93
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395