Detailed information NTG0780234

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PKO50_RS28575
• Genome accession: NZ_CP116775
• Coordinates: 6233017..6234162 (-)
• Length: 381 a.a.
• NCBI ID: WP_074977840.1
• Uniprot ID: -
• Organism: Pseudomonas sp. 273
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6228017..6239162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKO50_RS28555	-	6228017..6229288 (+)	1272	WP_109937107.1	dihydroorotase	-
PKO50_RS28560	-	6229641..6230813 (-)	1173	WP_275627906.1	cystathionine gamma-synthase	-
PKO50_RS28565	-	6230810..6232183 (-)	1374	WP_275627907.1	pyridoxal-phosphate dependent enzyme	-
PKO50_RS28570	-	6232472..6232876 (-)	405	WP_275627908.1	TM2 domain-containing protein	-
PKO50_RS28575	pilU	6233017..6234162 (-)	1146	WP_074977840.1	type IV pilus ATPase PilU	Machinery gene
PKO50_RS28580	pilT	6234265..6235299 (-)	1035	WP_109932577.1	type IV pilus twitching motility protein PilT	Machinery gene
PKO50_RS28585	-	6235373..6236062 (+)	690	WP_275627909.1	YggS family pyridoxal phosphate-dependent enzyme	-
PKO50_RS28590	proC	6236079..6236897 (+)	819	WP_275627910.1	pyrroline-5-carboxylate reductase	-
PKO50_RS28595	-	6236908..6237501 (+)	594	WP_089390951.1	YggT family protein	-
PKO50_RS28600	-	6237501..6237797 (+)	297	WP_089390952.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42671.03 Da
• Isoelectric Point: 6.7791

>NTDB_id=780234 PKO50_RS28575 WP_074977840.1 6233017..6234162(-) (pilU) [Pseudomonas sp. 273]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=780234 PKO50_RS28575 WP_074977840.1 6233017..6234162(-) (pilU) [Pseudomonas sp. 273]
ATGGAATTCGAGAAACTGCTGCGGCTGATGGTGGAGAAGGGCGCCTCTGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCCCCCGAGCAGACCCGCGAGACGGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGGCGC
TTCCGCGTCAGCGCTTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACACCTTCGAGGT
GGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACGCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACTCCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAAACCGACGCCGACCACCTGTCCAGCATGACCCAGGGCCTGACCCTGGAGCTGAGCGACG
ACGACCCCAACCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.927	100	0.879
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386