Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PML25_RS14015 Genome accession   NZ_CP116672
Coordinates   3388599..3389636 (-) Length   345 a.a.
NCBI ID   WP_006448885.1    Uniprot ID   A0A0G8M4X4
Organism   Xanthomonas hortorum pv. pelargonii strain 305     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3383599..3394636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML25_RS13990 (PML25_13990) ruvX 3384363..3384830 (-) 468 WP_006448879.1 Holliday junction resolvase RuvX -
  PML25_RS13995 (PML25_13995) - 3384823..3385389 (-) 567 WP_006448880.1 YqgE/AlgH family protein -
  PML25_RS14000 (PML25_14000) - 3385687..3386232 (+) 546 WP_168958904.1 DNA-3-methyladenine glycosylase I -
  PML25_RS14005 (PML25_14005) - 3386370..3387044 (-) 675 WP_168958926.1 YitT family protein -
  PML25_RS14010 (PML25_14010) pilU 3387346..3388476 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PML25_RS14015 (PML25_14015) pilT 3388599..3389636 (-) 1038 WP_006448885.1 type IV pilus twitching motility protein PilT Machinery gene
  PML25_RS14020 (PML25_14020) - 3389961..3390653 (+) 693 WP_168958905.1 YggS family pyridoxal phosphate-dependent enzyme -
  PML25_RS14025 (PML25_14025) proC 3390701..3391555 (+) 855 WP_168958906.1 pyrroline-5-carboxylate reductase -
  PML25_RS14030 (PML25_14030) - 3391993..3392418 (+) 426 WP_043889331.1 HU family DNA-binding protein -
  PML25_RS14035 (PML25_14035) - 3392559..3393173 (+) 615 WP_168958907.1 M23 family metallopeptidase -
  PML25_RS14040 (PML25_14040) - 3393265..3393573 (+) 309 WP_168958908.1 DUF3861 family protein -
  PML25_RS14045 (PML25_14045) - 3393794..3394180 (+) 387 WP_180336568.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38629.27 Da        Isoelectric Point: 6.6652

>NTDB_id=778996 PML25_RS14015 WP_006448885.1 3388599..3389636(-) (pilT) [Xanthomonas hortorum pv. pelargonii strain 305]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=778996 PML25_RS14015 WP_006448885.1 3388599..3389636(-) (pilT) [Xanthomonas hortorum pv. pelargonii strain 305]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAAAATAAGGCATCGGACCTGCATCTGTCGGCTGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGTTGGACCATAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGCGTCAACGCATTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGCACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGCTGATCGACCAGCCACAGGGGCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACGGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCCTTGCGCGAAGACCCGGACATCATTCTGGTCGGCGAATTACGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGCCATCTGGTGTTCGGTACCTTGCACACCAGCTCGGCGGCCAAGACCATTGACCGTATC
GTCGATGTGTTCCCGGCCGGCGAAAAACCGATGGTGCGCTCGATGCTGTCCGAATCCCTGCGCGCGGTGATTTCGCAGGC
GCTATTGAAGAAGGTCGGCGGCGGACGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCGATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAATACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8M4X4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.651

99.71

0.794
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394