Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PML25_RS14010 Genome accession   NZ_CP116672
Coordinates   3387346..3388476 (-) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hortorum pv. pelargonii strain 305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3382346..3393476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML25_RS13980 (PML25_13980) - 3382747..3383181 (-) 435 WP_006448877.1 OsmC family protein -
  PML25_RS13985 (PML25_13985) - 3383399..3384346 (-) 948 WP_168958903.1 aspartate carbamoyltransferase catalytic subunit -
  PML25_RS13990 (PML25_13990) ruvX 3384363..3384830 (-) 468 WP_006448879.1 Holliday junction resolvase RuvX -
  PML25_RS13995 (PML25_13995) - 3384823..3385389 (-) 567 WP_006448880.1 YqgE/AlgH family protein -
  PML25_RS14000 (PML25_14000) - 3385687..3386232 (+) 546 WP_168958904.1 DNA-3-methyladenine glycosylase I -
  PML25_RS14005 (PML25_14005) - 3386370..3387044 (-) 675 WP_168958926.1 YitT family protein -
  PML25_RS14010 (PML25_14010) pilU 3387346..3388476 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PML25_RS14015 (PML25_14015) pilT 3388599..3389636 (-) 1038 WP_006448885.1 type IV pilus twitching motility protein PilT Machinery gene
  PML25_RS14020 (PML25_14020) - 3389961..3390653 (+) 693 WP_168958905.1 YggS family pyridoxal phosphate-dependent enzyme -
  PML25_RS14025 (PML25_14025) proC 3390701..3391555 (+) 855 WP_168958906.1 pyrroline-5-carboxylate reductase -
  PML25_RS14030 (PML25_14030) - 3391993..3392418 (+) 426 WP_043889331.1 HU family DNA-binding protein -
  PML25_RS14035 (PML25_14035) - 3392559..3393173 (+) 615 WP_168958907.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=778995 PML25_RS14010 WP_006448884.1 3387346..3388476(-) (pilU) [Xanthomonas hortorum pv. pelargonii strain 305]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=778995 PML25_RS14010 WP_006448884.1 3387346..3388476(-) (pilU) [Xanthomonas hortorum pv. pelargonii strain 305]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTCAATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACGGCGCAACAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCATCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGACGTTTTCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTGGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCGACCGGGCACATCATCACT
ATCGAAGATCCGATCGAATTTGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCA
TGGACCACGCGGTGGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAATTGATTCCGACTCCGGATGGGCGCAGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTATGCCGATTCGCAGAACGA
AGTGCGTCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364