Detailed information NTG0001426

Experimentally validated

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: RS_RS05575
• Genome accession: NC_003295
• Coordinates: 1176593..1179136 (+)
• Length: 847 a.a.
• NCBI ID: WP_011001070.1
• Uniprot ID: Q8Y0C4
• Organism: Ralstonia pseudosolanacearum GMI1000
• Function: DNA uptake DNA binding and uptake

Function

Our results confirmed the critical role of the comA gene for natural transformation and that of recA for recombination and, more surprisingly, for the maintenance of an autonomous plasmid in the host cell.

Genomic Context

Location: 1171593..1184136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS_RS05555 (RS05756)	-	1172610..1173689 (-)	1080	WP_011001066.1	hypothetical protein	-
RS_RS05560 (RS05757)	-	1173762..1175012 (+)	1251	WP_011001067.1	lipoprotein-releasing ABC transporter permease subunit	-
RS_RS05565 (RS05758)	lolD	1175005..1175739 (+)	735	WP_011001068.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
RS_RS05570 (RS04635)	-	1175768..1176583 (+)	816	WP_011001069.1	TatD family hydrolase	-
RS_RS05575 (RS04634)	comA	1176593..1179136 (+)	2544	WP_011001070.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RS_RS05580 (RS04633)	-	1179245..1180726 (+)	1482	WP_016721677.1	circularly permuted type 2 ATP-grasp protein	-
RS_RS05585 (RS04632)	-	1180789..1181739 (+)	951	WP_011001072.1	alpha-E domain-containing protein	-
RS_RS05590 (RS04630)	-	1181797..1182633 (+)	837	WP_016725806.1	transglutaminase family protein	-
RS_RS05595 (RS04629)	-	1182717..1183631 (+)	915	WP_011001074.1	hypothetical protein	-

Sequence

Protein

• Length: 847 a.a.
• Molecular weight: 90736.53 Da
• Isoelectric Point: 10.4274

>NTDB_id=1426 RS_RS05575 WP_011001070.1 1176593..1179136(+) (comA) [Ralstonia pseudosolanacearum GMI1000]
MRALLIGFVAGCMALQTRPALAPVWQPALLGLACLLAMALLRRGARLPRLVPVMAVLAAACAGFGWSDWRAQQRLSVVLA
PAWEGKDIRATGVVAELPEVTEDATRFLFRIESSDAGDAVPPRVRLSWYGLRSWHERQREAEAATDRRAGIPDLQPGQRW
QLTLRLKRPHSLMNPGGFDGEYAMLADDVRAAGYVYTGKRARNALMGEADAGSGVGLRVERWRAAVRRHLLAALPEARYA
PVIVALVVGDQSGIAQADWERFRRTGISHLVSISGLHITMIAGLFGALWMALWRRSFGLARWLRTPLPLRMPAQRAGAVA
AMAAAFAYCLLAGMGVPAQRTLLMLATVAVARLADRSVPVSLSLCWAAALVALVDPWAVMSAGGWLSFGAVAVIFLAARI
VAADARDDVRRPAWPVRAWRGLRGAARVQLAVTFGLLPLTLLLFQQVSVVSAVANALAIPVVSFVTTPLALMGTALPEPW
SQPVLIWAEASFAWLMPWLDALALPGGSVWVAPAAPAWAWGLAVVGVPLLLVPGGYRAWAWRAQGAVLLLPMLLARAPAP
PPGEFRMVAFDIGQGAATLVETAGHRLLFDTGPRQGDLADAADRVIVPYLRGHGVQAIDTLVVSHEDSDHAGGTETVMAG
VPVRTMLASLPPGHGLERQARALGIEPAGCVAGQRWAWDGVAFEILYPVQVPSDRAAVSSNARSCVLRIANARYAAMLAG
DIGRAQEAALIAAEAPERLMADILLVPHHGSRTSSSGAFLDAVSAQVAVFQVGYRNRYGHPHPQVWQRYGARGIERLRTD
ATGAVVIETRGDALAVRTARSMRPRYWSSALEVAALAETTEPTGAQP

Nucleotide

• Length: 2544 bp

>NTDB_id=1426 RS_RS05575 WP_011001070.1 1176593..1179136(+) (comA) [Ralstonia pseudosolanacearum GMI1000]
ATGCGCGCCCTGCTGATCGGCTTCGTCGCAGGGTGCATGGCGCTGCAGACGCGGCCGGCGCTGGCGCCGGTGTGGCAGCC
GGCGCTGCTTGGGCTTGCTTGCCTGCTGGCGATGGCGTTGCTGCGGCGCGGCGCTCGGTTGCCCAGGCTCGTTCCCGTGA
TGGCAGTGCTGGCGGCGGCCTGCGCCGGCTTCGGCTGGAGCGATTGGCGCGCGCAGCAGCGCTTGTCCGTGGTGCTGGCG
CCCGCATGGGAGGGCAAGGACATCCGCGCGACGGGCGTCGTGGCCGAGCTGCCCGAAGTCACCGAGGACGCCACGCGCTT
CCTGTTCCGGATTGAATCCAGCGACGCGGGCGATGCCGTACCGCCGCGCGTGCGGCTGTCGTGGTACGGCCTGCGCAGCT
GGCATGAGCGGCAGCGGGAGGCGGAGGCCGCCACCGATCGCCGCGCCGGCATTCCCGATCTGCAGCCCGGCCAGCGCTGG
CAGCTGACGCTGCGGCTCAAGCGGCCGCACAGCCTGATGAATCCCGGCGGCTTCGATGGCGAATACGCGATGCTTGCCGA
CGATGTCCGTGCCGCGGGCTACGTGTACACGGGCAAACGCGCGCGCAATGCGTTGATGGGCGAAGCCGATGCCGGTTCCG
GTGTTGGGCTCCGTGTCGAGCGCTGGCGCGCCGCGGTGCGGCGGCATCTCCTGGCTGCGCTGCCCGAGGCACGCTACGCG
CCGGTGATCGTCGCCCTGGTGGTGGGCGACCAGAGCGGCATCGCGCAGGCGGATTGGGAGCGCTTCCGCCGCACCGGCAT
CAGCCATCTCGTCAGCATTTCCGGTCTGCACATCACGATGATCGCCGGGCTGTTCGGCGCGCTGTGGATGGCGCTGTGGC
GGCGCTCGTTCGGCCTGGCCCGCTGGCTGCGCACACCGCTGCCGCTGCGCATGCCGGCCCAGCGTGCGGGCGCCGTCGCC
GCCATGGCGGCGGCGTTTGCCTATTGCCTGCTGGCGGGGATGGGCGTGCCGGCGCAGCGCACGCTGCTGATGCTCGCGAC
CGTTGCCGTGGCGCGGCTGGCCGATCGCAGCGTGCCGGTCAGCCTGTCGCTGTGCTGGGCGGCGGCCTTGGTGGCGCTGG
TGGATCCGTGGGCGGTCATGTCGGCGGGAGGCTGGCTGTCGTTCGGCGCCGTGGCGGTCATCTTCCTGGCCGCGCGGATC
GTTGCCGCCGATGCGCGGGACGACGTCCGCCGGCCCGCCTGGCCGGTGCGCGCCTGGCGCGGCCTGCGCGGCGCCGCGCG
CGTCCAGCTGGCCGTGACGTTCGGCCTGCTGCCGTTGACCTTGCTGCTGTTCCAGCAGGTCTCGGTGGTGTCGGCCGTGG
CCAACGCGCTGGCGATCCCGGTGGTCAGCTTTGTCACGACGCCGCTGGCGCTGATGGGCACGGCGTTGCCCGAGCCATGG
TCGCAGCCGGTGCTGATATGGGCCGAGGCCAGCTTCGCCTGGCTCATGCCGTGGCTGGATGCGCTGGCGCTGCCCGGCGG
GTCGGTGTGGGTGGCGCCCGCCGCGCCGGCCTGGGCCTGGGGGCTGGCGGTGGTCGGTGTGCCGTTGCTGCTCGTGCCGG
GTGGGTATCGCGCCTGGGCCTGGCGGGCGCAGGGCGCGGTGCTGCTGCTGCCGATGCTGCTGGCACGCGCACCGGCGCCG
CCGCCGGGCGAGTTCCGCATGGTCGCATTCGACATCGGCCAGGGCGCCGCCACGCTGGTCGAGACGGCCGGCCACCGGCT
GCTGTTCGATACCGGTCCCCGCCAAGGCGACCTGGCCGATGCCGCCGATCGCGTGATCGTCCCGTATCTGCGCGGACATG
GGGTGCAGGCCATCGACACGCTGGTGGTCAGCCATGAGGATAGCGATCACGCCGGCGGCACCGAAACGGTGATGGCCGGT
GTGCCGGTGCGCACCATGCTGGCGTCCCTGCCGCCCGGCCACGGGCTGGAGCGGCAGGCCCGCGCGCTCGGCATCGAGCC
CGCAGGCTGCGTGGCCGGGCAGCGCTGGGCATGGGACGGCGTCGCCTTCGAAATCCTGTATCCGGTGCAGGTGCCCTCCG
ATCGGGCGGCCGTGTCCAGCAATGCCCGCAGCTGTGTGCTGCGCATCGCCAACGCGCGCTACGCCGCCATGCTGGCCGGC
GACATCGGCCGCGCCCAGGAAGCGGCGCTGATTGCCGCCGAAGCGCCGGAGCGGCTCATGGCAGATATCTTGCTTGTGCC
CCATCACGGCAGCAGGACGAGCTCCAGCGGCGCGTTCCTGGACGCGGTCTCTGCGCAGGTGGCGGTGTTCCAGGTCGGCT
ACCGCAACCGGTACGGTCATCCGCATCCGCAGGTCTGGCAGCGCTACGGCGCGCGCGGCATCGAGCGGCTGCGCACCGAT
GCCACCGGGGCCGTCGTCATCGAGACGCGCGGCGATGCGCTGGCGGTGCGGACGGCGCGGTCGATGCGGCCGCGCTACTG
GTCCTCGGCGCTGGAGGTGGCCGCCCTGGCAGAGACCACGGAGCCCACGGGCGCGCAGCCGTAA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (24-198)

• Beta-lactamase superfamily domain (Lactamase_B_2) (585-745)

• Competence protein (Competence) (246-532)

3D structure

• AlphaFold DB: Q8Y0C4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Pseudomonas stutzeri DSM 10701	37.29	100	0.39

References

• [1]: Anne Mercier et al. (2009) Influence of DNA conformation and role of comA and recA on natural transformation in Ralstonia solanacearum. Canadian Journal of Microbiology 55(6):762-70. [PMID: 19767847]