Detailed information NTG0001199

Experimentally validated

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: PSJM300_12650
• Genome accession: CP003725
• Coordinates: 2787039..2789264 (+)
• Length: 741 a.a.
• NCBI ID: AFN78592.1
• Uniprot ID: -
• Organism: Pseudomonas stutzeri DSM 10701
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

DNase-resistant state in the comA and exbB strains were similar to the wild type, suggesting that these proteins are involved in a late step of transformation, presumably in the translocation of DNA from the periplasm into the cytosol.

Genomic Context

Location: 2782039..2794264

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSJM300_12620	-	2782137..2782442 (-)	306	AFN78586.1	hypothetical protein	-
PSJM300_12625	-	2782445..2783023 (-)	579	AFN78587.1	hypothetical protein	-
PSJM300_12630	-	2783119..2784366 (+)	1248	AFN78588.1	LolC/E family lipoprotein releasing system, transmembrane protein	-
PSJM300_12635	lolD	2784374..2785072 (+)	699	AFN78589.1	lipoprotein transporter ATP-binding subunit	-
PSJM300_12640	-	2785072..2786316 (+)	1245	AFN78590.1	lipoprotein releasing system transmembrane protein LolE	-
PSJM300_12645	-	2786330..2786845 (-)	516	AFN78591.1	hypothetical protein	-
PSJM300_12650	comA	2787039..2789264 (+)	2226	AFN78592.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PSJM300_12655	exbB	2789336..2789968 (+)	633	AFN78593.1	MotA/TolQ/ExbB proton channel family protein	Machinery gene
PSJM300_12660	-	2789965..2790393 (+)	429	AFN78594.1	biopolymer transport protein ExbD/TolR	-
PSJM300_12665	lpxK	2790393..2791400 (+)	1008	AFN78595.1	tetraacyldisaccharide 4'-kinase	-
PSJM300_12670	-	2791446..2791631 (+)	186	AFN78596.1	hypothetical protein	-
PSJM300_12675	-	2791628..2792392 (+)	765	AFN78597.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
PSJM300_12680	-	2792395..2792859 (+)	465	AFN78598.1	protein tyrosine phosphatase	-
PSJM300_12685	murB	2792856..2793881 (+)	1026	AFN78599.1	UDP-N-acetylenolpyruvoylglucosamine reductase	-

Sequence

Protein

• Length: 741 a.a.
• Molecular weight: 80915.94 Da
• Isoelectric Point: 10.0542

>NTDB_id=1199 PSJM300_12650 AFN78592.1 2787039..2789264(+) (comA) [Pseudomonas stutzeri DSM 10701]
MRAGMLALAAGLLLLRFLPQLPSFGWLLALAPVGLLLLPFRWRPLAFFLFGLVWACLNAQWALDDRLPVDLDGRTFWLEG
QVTGLPDRRGDVVRFELEDIHSRHAGLPSRIRLAWYGGPEIRSGERWRLAARLKRPSGMVNPSAFDYEAWLLARRIGATG
TIKAGERIAEAASSGAWRDRLRQRLLTVEAHGRAGAIAALVVGDGSGLSTADWRVLQDTGTVHLMVISGQHVGMLAGLLY
AVVLLLARWGIWPQRLPWLPWACGLAFAGALGYGWLAGFEVPVRRACLMVAIVLLWRLRFRHLGVWLPLLIALVGVLMVD
PLASLQPGFWLSFVAVALLIWIFRGRLGVPSWWRALLYAQWAMALGLLPAMLVLGLPVSLSGPLANLLAVPWISVLVVPL
ALLGSFLLWVPWLGEALLWLAGGLLAVLFELLALIAGWQPAWLASALPLWAWSLVALGTLVLLLPAGVPLRVLGTLLLAP
LLFAPDSRPEQGRAEVWVFDVGQGLSVLVRTRDHALLYDAGPRYGDFDIGERVVFPSLRQLGLERLDLMMLSHADSDHAG
GALAIQRAMPVGAVLSGEPERLPAALDARSCRTGQRWTWNQVNFSVWRWPQATNGNQASCVLMVEAAGERLLLTGDIDAQ
AERALVDGGMEVNARWLLAPHHGSNSSSSAVFLAAVAADGALISRSSHNAFGHPHPAVLKRLQNAGAELHDTAEHGALSL
QLGAFGGARRMRDEPRFWREK

Nucleotide

• Length: 2226 bp

>NTDB_id=1199 PSJM300_12650 AFN78592.1 2787039..2789264(+) (comA) [Pseudomonas stutzeri DSM 10701]
ATGCGCGCAGGGATGCTGGCGCTCGCGGCAGGCCTGTTGCTGCTGCGCTTTCTGCCCCAATTGCCTTCGTTCGGCTGGCT
GCTGGCGCTCGCGCCGGTCGGCCTGCTGCTGTTGCCGTTTCGCTGGCGGCCGCTGGCGTTCTTCCTTTTCGGTCTGGTCT
GGGCCTGCCTCAACGCTCAATGGGCGCTCGATGACCGTTTGCCGGTAGACCTCGACGGGCGAACCTTCTGGCTGGAAGGG
CAGGTGACCGGGTTGCCGGACAGGCGCGGCGATGTGGTGCGGTTCGAACTGGAGGACATCCATTCCCGACATGCCGGCCT
GCCGTCCCGGATACGCCTGGCCTGGTATGGCGGGCCGGAAATCCGCAGCGGCGAACGTTGGCGCCTGGCGGCCAGGCTCA
AGCGTCCCAGCGGCATGGTCAATCCCAGCGCTTTCGATTACGAAGCCTGGCTACTGGCGCGGCGCATCGGTGCCACTGGC
ACCATCAAGGCGGGGGAGCGGATTGCCGAGGCGGCAAGCAGCGGTGCCTGGCGCGACCGCCTGCGCCAGCGGTTGCTGAC
GGTCGAGGCCCATGGCCGAGCGGGCGCCATCGCCGCGCTGGTGGTGGGCGATGGCTCGGGTCTGTCCACGGCCGATTGGC
GGGTTCTGCAGGATACCGGCACGGTGCACCTGATGGTCATCTCGGGCCAGCACGTCGGCATGCTGGCCGGCCTCCTTTAT
GCGGTGGTGCTTTTGCTGGCTCGCTGGGGTATCTGGCCGCAGCGTCTGCCCTGGCTGCCCTGGGCGTGCGGACTGGCCTT
CGCCGGTGCGCTCGGCTACGGCTGGCTGGCGGGCTTCGAAGTACCTGTCCGACGGGCCTGCCTGATGGTGGCGATCGTCC
TGTTATGGCGCCTGCGCTTTCGCCACCTTGGCGTCTGGCTGCCGCTGCTGATCGCGCTGGTCGGGGTGCTAATGGTCGAT
CCGCTGGCCAGTCTGCAGCCCGGTTTCTGGTTGTCGTTCGTCGCGGTGGCGTTGCTGATCTGGATCTTCCGTGGTCGGCT
CGGCGTTCCCTCATGGTGGCGGGCGTTGCTGTACGCGCAATGGGCCATGGCGCTGGGGTTGTTGCCGGCGATGCTGGTGC
TGGGCCTGCCGGTCAGCCTGAGCGGGCCGCTGGCGAATCTGCTGGCCGTTCCCTGGATCAGCGTGCTGGTCGTTCCGCTG
GCCTTGCTGGGCAGTTTCCTGTTGTGGGTTCCCTGGCTTGGCGAGGCCTTACTCTGGCTCGCCGGCGGCTTGCTGGCGGT
TCTGTTCGAACTTCTGGCGCTGATCGCTGGCTGGCAGCCCGCCTGGCTGGCGTCCGCGCTGCCGTTGTGGGCGTGGAGCC
TCGTGGCACTGGGCACCCTGGTCCTGCTGCTGCCCGCCGGTGTGCCGTTGCGGGTGCTGGGCACGCTTCTGCTGGCGCCG
CTGCTGTTCGCGCCCGATAGCAGGCCGGAGCAAGGTCGTGCCGAGGTCTGGGTGTTCGATGTGGGGCAGGGACTTTCGGT
ACTGGTACGCACCCGGGATCATGCGCTGCTGTATGACGCAGGACCGCGCTACGGCGATTTCGATATCGGTGAGCGGGTGG
TATTTCCTTCGTTGCGCCAACTGGGGCTGGAGCGGCTGGACCTGATGATGCTCAGCCACGCCGACAGTGATCATGCCGGC
GGGGCGCTCGCAATCCAGCGGGCCATGCCGGTTGGCGCCGTGCTCAGCGGCGAACCCGAACGACTGCCGGCAGCGCTGGA
TGCCCGCTCCTGTCGGACCGGACAGCGCTGGACATGGAATCAGGTCAATTTCAGTGTCTGGCGCTGGCCGCAGGCGACCA
ACGGCAACCAGGCCTCCTGCGTGTTGATGGTCGAGGCGGCCGGCGAACGGCTGCTGCTCACCGGCGATATTGACGCGCAG
GCCGAGCGCGCGCTGGTCGATGGCGGAATGGAGGTGAACGCCCGATGGTTGCTGGCGCCACACCATGGCAGCAACAGCTC
CTCGTCCGCTGTGTTTCTGGCAGCCGTGGCAGCGGATGGCGCACTGATTTCACGCAGCAGTCACAACGCGTTCGGCCATC
CGCATCCGGCGGTGCTCAAGCGGCTGCAAAACGCGGGCGCGGAACTCCACGATACGGCCGAGCACGGTGCGCTGAGCCTG
CAGCTGGGTGCGTTCGGCGGTGCGCGAAGAATGCGTGACGAACCACGCTTCTGGCGGGAAAAATGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (200-464)

• Domain of unknown function (DUF4131) (DUF4131) (19-167)

• Metallo-beta-lactamase superfamily (Lactamase_B) (501-687)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Ralstonia pseudosolanacearum GMI1000	37.29	100	0.39

References

• [1]: Petra Meier et al. (2002) Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA. FEMS Microbiology Letters 207(1):75-80. [PMID: 11886754]
• [2]: S Graupner et al. (2001) Identification and characterization of novel competence genes comA and exbB involved in natural genetic transformation of Pseudomonas stutzeri. Research in Microbiology 152(5):451-60. [PMID: 11446513]