Detailed information    

experimental Experimentally validated

Overview

Name   exbB   Type   Machinery gene
Locus tag   PSJM300_12655 Genome accession   CP003725
Coordinates   2789336..2789968 (+) Length   210 a.a.
NCBI ID   AFN78593.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

Insertional inactivation of exbB decreased the transformability to 20% of that of the wild type. DNase-resistant state in the comA and exbB strains were similar to the wild type, suggesting that these proteins are involved in a late step of transformation, presumably in the translocation of DNA from the periplasm into the cytosol.


Genomic Context

Location: 2784336..2794968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_12635 lolD 2784374..2785072 (+) 699 AFN78589.1 lipoprotein transporter ATP-binding subunit -
  PSJM300_12640 - 2785072..2786316 (+) 1245 AFN78590.1 lipoprotein releasing system transmembrane protein LolE -
  PSJM300_12645 - 2786330..2786845 (-) 516 AFN78591.1 hypothetical protein -
  PSJM300_12650 comA 2787039..2789264 (+) 2226 AFN78592.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PSJM300_12655 exbB 2789336..2789968 (+) 633 AFN78593.1 MotA/TolQ/ExbB proton channel family protein Machinery gene
  PSJM300_12660 - 2789965..2790393 (+) 429 AFN78594.1 biopolymer transport protein ExbD/TolR -
  PSJM300_12665 lpxK 2790393..2791400 (+) 1008 AFN78595.1 tetraacyldisaccharide 4'-kinase -
  PSJM300_12670 - 2791446..2791631 (+) 186 AFN78596.1 hypothetical protein -
  PSJM300_12675 - 2791628..2792392 (+) 765 AFN78597.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  PSJM300_12680 - 2792395..2792859 (+) 465 AFN78598.1 protein tyrosine phosphatase -
  PSJM300_12685 murB 2792856..2793881 (+) 1026 AFN78599.1 UDP-N-acetylenolpyruvoylglucosamine reductase -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 22754.07 Da        Isoelectric Point: 7.8873

>NTDB_id=1200 PSJM300_12655 AFN78593.1 2789336..2789968(+) (exbB) [Pseudomonas stutzeri DSM 10701]
MWELVKAGGWIMLPIILCSIAALGIIVERLWTLRPSRVTPPHLLGQVWKWIKDKQLSNQKLKELRADSPLGEILAAGLAN
SKRGREIMKECIEEAAARVVHDLERYLNALGTIAGIAPLLGLLGTVLGMIEIFSAFMGSGMANAPLLAGGISKALITTAA
GLMVGIPALFFHRFLLRRVDELVVGMEQEAIKLVEVVQGDRDVDLGEGKA

Nucleotide


Download         Length: 633 bp        

>NTDB_id=1200 PSJM300_12655 AFN78593.1 2789336..2789968(+) (exbB) [Pseudomonas stutzeri DSM 10701]
GTGTGGGAGCTGGTCAAAGCGGGCGGCTGGATAATGCTGCCGATCATTCTGTGTTCCATCGCGGCACTGGGCATCATCGT
CGAGCGCCTGTGGACATTGCGGCCTAGCCGCGTCACGCCTCCGCATCTGCTCGGGCAGGTATGGAAGTGGATCAAGGACA
AACAGCTCAGCAACCAGAAGCTCAAGGAGTTGCGTGCCGACTCGCCGCTGGGGGAAATTCTGGCCGCCGGCCTGGCCAAC
TCCAAGCGTGGTCGAGAGATCATGAAGGAATGCATCGAGGAGGCGGCTGCTCGTGTCGTGCATGATCTGGAGCGTTACCT
CAACGCGCTGGGCACCATCGCCGGCATCGCCCCCCTGCTGGGCCTGCTCGGGACGGTGCTGGGGATGATCGAGATCTTCA
GCGCCTTCATGGGCTCGGGAATGGCCAATGCGCCCTTGCTGGCCGGCGGTATCTCCAAGGCCCTGATCACCACCGCGGCG
GGCCTGATGGTCGGCATACCGGCCTTGTTCTTCCATCGCTTCCTGCTGCGTCGTGTCGACGAGCTGGTGGTGGGCATGGA
ACAGGAAGCCATCAAGCTGGTGGAAGTGGTACAGGGCGACCGTGACGTCGATCTGGGTGAGGGCAAGGCGTGA

Domains

Predicted by InterproScan.

MotA/TolQ/ExbB proton channel family (MotA_ExbB)

(66-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S Graupner et al. (2001) Identification and characterization of novel competence genes comA and exbB involved in natural genetic transformation of Pseudomonas stutzeri. Research in Microbiology 152(5):451-60. [PMID: 11446513]