Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PJU76_RS11415 Genome accession   NZ_CP116458
Coordinates   2277412..2278461 (-) Length   349 a.a.
NCBI ID   WP_064219958.1    Uniprot ID   A0A179B867
Organism   Acidithiobacillus ferriphilus strain YL25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2272412..2283461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJU76_RS11385 (PJU76_11385) trmB 2272455..2273138 (+) 684 WP_271779608.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  PJU76_RS11390 (PJU76_11390) - 2273441..2273692 (+) 252 WP_271779609.1 hypothetical protein -
  PJU76_RS11395 (PJU76_11395) - 2273877..2274428 (+) 552 WP_271779610.1 hypothetical protein -
  PJU76_RS11400 (PJU76_11400) - 2274572..2274914 (+) 343 Protein_2243 type II toxin-antitoxin system YhaV family toxin -
  PJU76_RS11405 (PJU76_11405) - 2275103..2276290 (-) 1188 WP_271779612.1 glycosyltransferase -
  PJU76_RS11410 (PJU76_11410) pilU 2276272..2277402 (-) 1131 WP_271779613.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJU76_RS11415 (PJU76_11415) pilT 2277412..2278461 (-) 1050 WP_064219958.1 type IV pilus twitching motility protein PilT Machinery gene
  PJU76_RS11420 (PJU76_11420) - 2278562..2279233 (+) 672 WP_271779614.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJU76_RS11425 (PJU76_11425) proC 2279230..2280057 (+) 828 WP_215861232.1 pyrroline-5-carboxylate reductase -
  PJU76_RS11430 (PJU76_11430) - 2280054..2280332 (+) 279 WP_064219961.1 YggT family protein -
  PJU76_RS11435 (PJU76_11435) - 2280360..2280710 (+) 351 WP_271779615.1 DUF167 domain-containing protein -
  PJU76_RS11440 (PJU76_11440) bioB 2280707..2281657 (+) 951 WP_215861233.1 biotin synthase BioB -
  PJU76_RS11445 (PJU76_11445) - 2281699..2282268 (-) 570 WP_215880127.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38466.03 Da        Isoelectric Point: 6.7267

>NTDB_id=777477 PJU76_RS11415 WP_064219958.1 2277412..2278461(-) (pilT) [Acidithiobacillus ferriphilus strain YL25]
MDISELLAFAVKNNASDTHISAGLSPMLRINGDIRPLNVEAQDRKAVHGMIYDIMNDSQRKGYEENLEIDFAYELPGVAR
FRVNAFNQDRGPAAAFRTIPAKVLSLEDLNAPKSFMEIINVPRGLVLVTGPTGSGKSTTLAAMVDHINANRADHIITIED
PIEFLHTPKKCLVNQREVGANTHSFEHALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVAAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKVTREDALRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=777477 PJU76_RS11415 WP_064219958.1 2277412..2278461(-) (pilT) [Acidithiobacillus ferriphilus strain YL25]
ATGGACATTTCAGAACTGCTTGCTTTCGCGGTGAAAAACAATGCTTCGGACACGCATATCTCGGCGGGTTTGTCGCCCAT
GCTGCGGATCAACGGCGATATCCGCCCTCTCAATGTCGAGGCGCAGGACAGAAAGGCCGTACATGGCATGATTTACGACA
TCATGAATGATTCCCAGCGGAAGGGGTATGAGGAAAATCTGGAAATCGACTTTGCCTATGAACTGCCCGGCGTGGCGCGT
TTTCGGGTTAACGCCTTCAACCAGGACCGTGGGCCCGCGGCGGCGTTCCGGACGATTCCCGCCAAGGTGCTGAGTCTGGA
AGATCTCAATGCACCCAAGTCTTTTATGGAGATCATCAACGTACCGCGCGGACTGGTGCTGGTCACCGGACCGACGGGGT
CGGGTAAATCAACGACGCTGGCAGCGATGGTGGATCATATCAATGCGAACCGCGCCGACCATATTATCACCATTGAAGAT
CCCATCGAGTTTTTGCACACTCCCAAGAAGTGTCTCGTCAACCAGCGCGAGGTAGGCGCCAACACCCATTCCTTTGAACA
TGCCCTGCGCTCAGCCTTACGTGAAGATCCGGATATTATTCTGGTGGGCGAATTGCGTGACTTGGAGACCATGCGCCTGG
CTCTGACTGCGGCGGAAACGGGCCATATCGTGTTTGCGACGCTGCATACCAGTTCGGCGCCGAAAACCATCGACCGTATT
GTCGACTCCTTTCCTGGGGGGGAAAAGGATATGGTTCGCGCGATGCTGTCAGAATCCCTGCGCGCGGTTATTTCTCAGAC
CCTGCTCAAAACGGCCGATGGTAAAGGCCGAGTCGCCGCGCATGAAATTATGATTGCCACACCGGCCATCCGAAACCTCA
TTCGCGAAAATAAGGTGGCGCAGATGTACTCGGTGATCCAGACCGGACAAAATCAGGGTATGCAAACTCTCGACCAGTGC
CTTGCCGACCTGGTGCGTGCCCACAAAGTAACCCGCGAAGATGCCTTGCGTAGGGTTCAGAACAAAGACAGCTTTATGAA
TGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A179B867

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

68.497

99.14

0.679
  pilT Acinetobacter baumannii D1279779

68.497

99.14

0.679
  pilT Acinetobacter baumannii strain A118

68.497

99.14

0.679
  pilT Pseudomonas stutzeri DSM 10701

68.406

98.854

0.676
  pilT Pseudomonas aeruginosa PAK

67.536

98.854

0.668
  pilT Acinetobacter baylyi ADP1

70.462

93.123

0.656
  pilT Legionella pneumophila strain Lp02

65.797

98.854

0.65
  pilT Legionella pneumophila strain ERS1305867

65.797

98.854

0.65
  pilT Neisseria meningitidis 8013

65.217

98.854

0.645
  pilT Neisseria gonorrhoeae MS11

64.928

98.854

0.642
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.308

93.123

0.636
  pilT Vibrio cholerae strain A1552

68.308

93.123

0.636
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.57

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.56

96.275

0.41
  pilU Acinetobacter baylyi ADP1

42.308

96.848

0.41
  pilU Vibrio cholerae strain A1552

39.881

96.275

0.384