Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PJU76_RS11410 Genome accession   NZ_CP116458
Coordinates   2276272..2277402 (-) Length   376 a.a.
NCBI ID   WP_271779613.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain YL25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2271272..2282402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJU76_RS11380 (PJU76_11380) purE 2271907..2272404 (-) 498 WP_215849030.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  PJU76_RS11385 (PJU76_11385) trmB 2272455..2273138 (+) 684 WP_271779608.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  PJU76_RS11390 (PJU76_11390) - 2273441..2273692 (+) 252 WP_271779609.1 hypothetical protein -
  PJU76_RS11395 (PJU76_11395) - 2273877..2274428 (+) 552 WP_271779610.1 hypothetical protein -
  PJU76_RS11400 (PJU76_11400) - 2274572..2274914 (+) 343 Protein_2243 type II toxin-antitoxin system YhaV family toxin -
  PJU76_RS11405 (PJU76_11405) - 2275103..2276290 (-) 1188 WP_271779612.1 glycosyltransferase -
  PJU76_RS11410 (PJU76_11410) pilU 2276272..2277402 (-) 1131 WP_271779613.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJU76_RS11415 (PJU76_11415) pilT 2277412..2278461 (-) 1050 WP_064219958.1 type IV pilus twitching motility protein PilT Machinery gene
  PJU76_RS11420 (PJU76_11420) - 2278562..2279233 (+) 672 WP_271779614.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJU76_RS11425 (PJU76_11425) proC 2279230..2280057 (+) 828 WP_215861232.1 pyrroline-5-carboxylate reductase -
  PJU76_RS11430 (PJU76_11430) - 2280054..2280332 (+) 279 WP_064219961.1 YggT family protein -
  PJU76_RS11435 (PJU76_11435) - 2280360..2280710 (+) 351 WP_271779615.1 DUF167 domain-containing protein -
  PJU76_RS11440 (PJU76_11440) bioB 2280707..2281657 (+) 951 WP_215861233.1 biotin synthase BioB -
  PJU76_RS11445 (PJU76_11445) - 2281699..2282268 (-) 570 WP_215880127.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41651.07 Da        Isoelectric Point: 5.7767

>NTDB_id=777476 PJU76_RS11410 WP_271779613.1 2276272..2277402(-) (pilU) [Acidithiobacillus ferriphilus strain YL25]
MSVLDDLLVLMVEKNGSDLYLTVGSPPVMKIDGKAVSLGTEMLKPSQVLNLAKEILGMERLQEFQREKEINMAISAPGIG
RFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKDLAMTSRGLVLFVGATGSGKSTSLASMIQYRNQNAAGHILTIE
DPIEFLHKNAMSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLRAVVSQRLLPIKGQSGRVAAIEVLINTPAIADLIYKGEVGLLKDAMARTNDVGMQTFDQ
SLRQLFMDGLVEYEDALRGADSQNDLRLAIKQECMRRGVEDPGASTSAEGQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=777476 PJU76_RS11410 WP_271779613.1 2276272..2277402(-) (pilU) [Acidithiobacillus ferriphilus strain YL25]
ATGTCTGTGCTGGATGATCTGCTGGTCTTGATGGTGGAAAAAAATGGCTCTGACCTTTACCTCACCGTAGGCTCGCCGCC
GGTCATGAAAATTGACGGGAAGGCGGTTTCGTTAGGGACCGAGATGCTGAAGCCGAGTCAGGTGCTGAATCTGGCCAAAG
AAATTCTGGGCATGGAGCGCTTGCAGGAATTCCAGCGGGAAAAAGAAATTAATATGGCCATCTCTGCGCCCGGGATTGGG
CGTTTCCGGGTCAATGGCTTTTTTCAGCGCGGTGAACTGAGCTTTGTCTTGCGCGCCATCAAGACGCAAATTCCCAGCCT
GGAACAGCTCAGGCTGCCGCCCATACTGAAAGACCTGGCCATGACTTCCCGGGGCCTGGTGCTCTTCGTCGGGGCTACCG
GCTCTGGTAAAAGTACCTCTCTGGCGTCGATGATTCAGTATCGCAATCAGAATGCGGCGGGCCATATTCTCACGATTGAG
GACCCCATTGAATTTCTTCACAAGAATGCGATGTCTATCGTTAATCAACGCGAAGTGGGTATAGATACCCTGAATTACGA
GCGGGCGCTGGAGAACGCCTTGCGTGAGGCGCCAGATGTTATTCTGATCGGCGAAGTGCGTAGTCGTGATACCATGGATC
ATGCCATGGCGTATGCAGAAACCGGACACTTGTGTATGTCGACCTTACATGCGAACAATGCTAATCAGGCCATTGAACGT
ATCATCAACTTTTTCCCGGAGGACCGTAAAAAACAATTGTTGATGGATCTTTCACTCAATTTGCGCGCCGTGGTCTCACA
ACGCCTGTTGCCGATCAAGGGGCAGAGCGGGCGAGTCGCCGCTATAGAGGTGCTGATCAATACCCCGGCCATTGCCGATT
TGATCTACAAGGGTGAAGTGGGCTTGCTGAAAGACGCCATGGCGCGGACGAATGATGTGGGTATGCAGACGTTTGATCAG
AGCCTGCGACAACTGTTTATGGATGGGCTGGTGGAATATGAAGACGCCCTGCGCGGCGCCGATTCGCAGAATGACCTGCG
CCTTGCCATCAAGCAGGAGTGCATGCGGCGTGGCGTAGAGGACCCTGGTGCCAGTACTTCTGCAGAAGGGCAGTGGCGCA
TTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.632

92.553

0.561
  pilU Vibrio cholerae strain A1552

52.893

96.543

0.511
  pilU Acinetobacter baylyi ADP1

54.441

92.819

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.228

92.287

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.545

87.766

0.391
  pilT Vibrio cholerae strain A1552

44.545

87.766

0.391
  pilT Acinetobacter baylyi ADP1

42.042

88.564

0.372
  pilT Legionella pneumophila strain Lp02

41.791

89.096

0.372
  pilT Legionella pneumophila strain ERS1305867

41.791

89.096

0.372
  pilT Pseudomonas aeruginosa PAK

40.841

88.564

0.362
  pilT Neisseria meningitidis 8013

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

40.356

89.628

0.362
  pilT Acinetobacter baumannii strain A118

40.356

89.628

0.362
  pilT Acinetobacter nosocomialis M2

40.356

89.628

0.362