Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PL263_RS15985 Genome accession   NZ_CP116398
Coordinates   3561057..3562094 (+) Length   345 a.a.
NCBI ID   WP_054759731.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3556057..3567094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15965 (PL263_15960) hisC 3556157..3557236 (-) 1080 WP_278210294.1 histidinol-phosphate transaminase -
  PL263_RS15970 (PL263_15965) - 3557517..3559028 (-) 1512 WP_278210295.1 TIM44-like domain-containing protein -
  PL263_RS15975 (PL263_15970) - 3559025..3559648 (-) 624 WP_278210296.1 hypothetical protein -
  PL263_RS15980 (PL263_15975) - 3559861..3560883 (+) 1023 WP_140913463.1 SPFH domain-containing protein -
  PL263_RS15985 (PL263_15980) pilT 3561057..3562094 (+) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  PL263_RS15990 (PL263_15985) pilU 3562111..3563244 (+) 1134 WP_278210298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PL263_RS15995 (PL263_15990) mltB 3563263..3564417 (+) 1155 WP_278210299.1 lytic murein transglycosylase B -
  PL263_RS16000 (PL263_15995) - 3564565..3565011 (+) 447 WP_278210300.1 hypothetical protein -
  PL263_RS16005 (PL263_16000) dinB 3565040..3566149 (-) 1110 WP_278210301.1 DNA polymerase IV -
  PL263_RS16010 (PL263_16005) - 3566156..3566458 (-) 303 WP_140913467.1 DUF4389 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38324.18 Da        Isoelectric Point: 7.0362

>NTDB_id=777142 PL263_RS15985 WP_054759731.1 3561057..3562094(+) (pilT) [Methylomonas sp. EFPC3]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELDAPKFFQELCTKPRGLILVTGPTGSGKSTTLAAMINHINNNDYAHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=777142 PL263_RS15985 WP_054759731.1 3561057..3562094(+) (pilT) [Methylomonas sp. EFPC3]
ATGGATATCGCCGAACTGTTGACGTTCTCCGTCAAGAATAAAGCTTCCGACTTGCACCTTTCCGCCGGCTTGCCGCCGAT
GATCCGGGTGGACGGCGACATTCGCCGGATCAACATCCCGGCCCTGAACCACAAGGAAGTGCACGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCGCGATTACGAGGAGTTTCTGGAAACCGACTTTTCGTTTGCCTTGCCGGGGGTAGCCCGA
TTCCGGGTCAACGCCTTCAATCAGGATCGCGGTGCCGGCGCGGTATTCCGGACCATTCCGTCGAAAGTCTTGACCCTGGA
AGAGCTGGATGCGCCCAAGTTTTTTCAGGAGCTGTGTACCAAGCCGCGCGGTTTGATTTTGGTGACCGGACCGACCGGTT
CCGGTAAATCCACCACGTTGGCGGCGATGATCAACCACATCAACAACAACGATTACGCCCACATCCTCACGGTCGAAGAT
CCGATCGAGTTTGTTCACGAAAGCCAGAAATCGCTGATTAACCAGCGCGAGGTGCATCGCGATACGCTGGGTTTCAACGA
AGCCTTGCGCTCGGCGTTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGATTTGGAAACCATTCGTCTGG
CGCTGACTGCGGCCGAAACCGGCCACTTGGTGTTCGGCACCTTGCACACCACTTCGGCCGCCAAGACCATAGACCGGATC
ATCGACGTGTTCCCGGCGGCCGAGAAAGACATGATCCGGGCCATGTTGTCCGAGTCGTTACAGGCGGTTATTTCGCAAAC
ACTGCTGAAGAAGGTCGGCGGCGGCCGGATCGCTGCGCATGAGATCATGGTCGGTACCCCGGCGATCCGCAACCTGATCC
GTGAAGCCAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGGCGCAAGGACGGCATGCAGACGCTGGACCAAAACCTG
AAAGAGCTGGTCGACAAGGGCCTGATTACCGCCAAGGCGGCGATGACTCGGGCCGTCAATAAAGACATGTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

81.232

98.841

0.803
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria gonorrhoeae MS11

67.638

99.42

0.672
  pilT Neisseria meningitidis 8013

67.638

99.42

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4