Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PL263_RS15990 Genome accession   NZ_CP116398
Coordinates   3562111..3563244 (+) Length   377 a.a.
NCBI ID   WP_278210298.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3557111..3568244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15970 (PL263_15965) - 3557517..3559028 (-) 1512 WP_278210295.1 TIM44-like domain-containing protein -
  PL263_RS15975 (PL263_15970) - 3559025..3559648 (-) 624 WP_278210296.1 hypothetical protein -
  PL263_RS15980 (PL263_15975) - 3559861..3560883 (+) 1023 WP_140913463.1 SPFH domain-containing protein -
  PL263_RS15985 (PL263_15980) pilT 3561057..3562094 (+) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  PL263_RS15990 (PL263_15985) pilU 3562111..3563244 (+) 1134 WP_278210298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PL263_RS15995 (PL263_15990) mltB 3563263..3564417 (+) 1155 WP_278210299.1 lytic murein transglycosylase B -
  PL263_RS16000 (PL263_15995) - 3564565..3565011 (+) 447 WP_278210300.1 hypothetical protein -
  PL263_RS16005 (PL263_16000) dinB 3565040..3566149 (-) 1110 WP_278210301.1 DNA polymerase IV -
  PL263_RS16010 (PL263_16005) - 3566156..3566458 (-) 303 WP_140913467.1 DUF4389 domain-containing protein -
  PL263_RS16015 (PL263_16010) - 3566469..3567491 (-) 1023 WP_140913468.1 DUF2333 family protein -
  PL263_RS16020 (PL263_16015) - 3567576..3567848 (-) 273 WP_278210302.1 YcgL domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42018.25 Da        Isoelectric Point: 6.6669

>NTDB_id=777143 PL263_RS15990 WP_278210298.1 3562111..3563244(+) (pilU) [Methylomonas sp. EFPC3]
MDFTALLALMVEKRASDLFITAGRPPSMKVNGKVVEVSKTSLTSEQTLKLVLGIMSQRQRDEFENTKECQFALSAHSLGR
FRVSAFTQRDSAGMVLRRIETTIPTAEELHLPPVLKELIMHKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHQGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMQHAITFAETGHLCVCTLHANNANQAIDRI
LHFFPEDMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLVADLIRKGEVHKLKELMKSSREHGMQTFDQA
LYDLYTAGKISYEDALHAADSRNEVRLMIKLGAENVNFETAGMTLAESDEDGGSLYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=777143 PL263_RS15990 WP_278210298.1 3562111..3563244(+) (pilU) [Methylomonas sp. EFPC3]
ATGGACTTTACCGCGCTATTGGCATTGATGGTGGAAAAACGGGCGTCGGATTTATTCATTACCGCCGGGCGGCCGCCGTC
GATGAAAGTCAACGGCAAAGTCGTCGAAGTCTCGAAGACGTCGCTGACCAGCGAACAAACCCTGAAGCTGGTGCTCGGCA
TCATGTCGCAACGCCAGCGCGACGAATTCGAAAACACCAAGGAATGCCAATTCGCGTTAAGCGCGCACAGTCTGGGCCGG
TTCCGGGTCAGCGCCTTCACCCAGCGCGATTCGGCCGGCATGGTGTTGCGCCGGATCGAAACCACGATTCCGACTGCGGA
AGAGTTGCATCTGCCGCCGGTGTTGAAGGAATTGATCATGCACAAGCGCGGCTTGGTCATGTTCGTCGGTGCGACCGGCA
CCGGTAAATCGACCTCGCTCGCGGCCTTGATCCGCCACCGCAACGAAAACAGCAGCGGCCACATCATTTCCATCGAAGAT
CCGATCGAGTTCATTCACCCCCACCAGGGCTGTATCATTACCCAGCGCGAAGTGGGTCTGGATACCGAATCGTTCGAAGT
CGCTTTGAAAAACACACTGCGTCAAGCGCCGGACGTGATTCTAATCGGCGAGGTCAGGACCCGCGAGACCATGCAACACG
CGATAACCTTTGCCGAGACCGGCCACCTGTGCGTCTGTACCTTGCACGCCAACAACGCCAACCAGGCCATAGACCGCATC
CTGCACTTTTTCCCGGAAGACATGCACAGCCAGTTGTTCATGGACTTGTCTTTGAATTTGCGCGGCATCGTCGCTCAGCA
ATTGATTCAGCGTGCTGATGGCAAAGGCCGTTATCCCGCCATCGAAATTCTGTTGAATACGCCGCTAGTCGCCGATTTGA
TTCGTAAGGGCGAGGTGCATAAATTAAAAGAATTGATGAAGAGCTCGCGCGAGCATGGCATGCAAACTTTCGACCAGGCG
CTTTACGATCTGTACACAGCCGGTAAAATTAGTTACGAAGATGCTCTGCACGCTGCCGACTCCCGCAACGAAGTCCGCCT
GATGATCAAATTGGGCGCCGAGAACGTCAATTTCGAAACCGCCGGCATGACGTTGGCCGAAAGCGACGAAGACGGAGGCA
GCCTTTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.766

100

0.695
  pilU Acinetobacter baylyi ADP1

63.944

94.164

0.602
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.54

92.308

0.411
  pilT Legionella pneumophila strain Lp02

42.183

89.92

0.379
  pilT Legionella pneumophila strain ERS1305867

42.183

89.92

0.379
  pilT Pseudomonas aeruginosa PAK

41.298

89.92

0.371
  pilT Acinetobacter baumannii D1279779

41.003

89.92

0.369
  pilT Acinetobacter baumannii strain A118

41.003

89.92

0.369
  pilT Acinetobacter nosocomialis M2

41.003

89.92

0.369
  pilT Acinetobacter baylyi ADP1

40.469

90.451

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

88.594

0.363
  pilT Vibrio cholerae strain A1552

41.018

88.594

0.363