Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PK654_RS02255 Genome accession   NZ_CP116383
Coordinates   448093..449133 (+) Length   346 a.a.
NCBI ID   WP_271697451.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43137     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443093..454133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PK654_RS02225 (PK654_02225) hemW 443847..445001 (-) 1155 WP_271697445.1 radical SAM family heme chaperone HemW -
  PK654_RS02230 (PK654_02230) - 445005..445601 (-) 597 WP_271697446.1 XTP/dITP diphosphatase -
  PK654_RS02235 (PK654_02235) yggU 445635..445922 (-) 288 WP_271697447.1 DUF167 family protein YggU -
  PK654_RS02240 (PK654_02240) - 445922..446479 (-) 558 WP_271697448.1 YggT family protein -
  PK654_RS02245 (PK654_02245) proC 446516..447334 (-) 819 WP_271697449.1 pyrroline-5-carboxylate reductase -
  PK654_RS02250 (PK654_02250) - 447358..448068 (-) 711 WP_271697450.1 YggS family pyridoxal phosphate-dependent enzyme -
  PK654_RS02255 (PK654_02255) pilT 448093..449133 (+) 1041 WP_271697451.1 type IV pilus twitching motility protein PilT Machinery gene
  PK654_RS02260 (PK654_02260) pilU 449145..450269 (+) 1125 WP_271697452.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PK654_RS02265 (PK654_02265) ruvX 450291..450716 (-) 426 WP_271697453.1 Holliday junction resolvase RuvX -
  PK654_RS02270 (PK654_02270) - 450767..451330 (-) 564 WP_271697454.1 YqgE/AlgH family protein -
  PK654_RS02275 (PK654_02275) gshB 451389..452336 (-) 948 WP_271697455.1 glutathione synthase -
  PK654_RS02280 (PK654_02280) rsmE 452349..453080 (-) 732 WP_271697456.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PK654_RS02285 (PK654_02285) - 453217..453717 (-) 501 WP_271697457.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38058.64 Da        Isoelectric Point: 5.9296

>NTDB_id=776846 PK654_RS02255 WP_271697451.1 448093..449133(+) (pilT) [Vibrio sp. SCSIO 43137]
MDISELLEFSVKHNASDLHLSAGVPPMIRIDGDVRKLGVPALEHSDVHRMVFDIMTDAQKSEFEGNLEVDFSFELPQIGR
FRVNAFNQSRGCSAVFRTIPTDIPTLQELESPAIFNDIAEMEKGLVLVTGPTGSGKSTTLAAMVDHVNQNFNKHILTIED
PIEFLHSNKKCLINQREVHKDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKAMVRSMLSESLRAVIAQRLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAASGMVTMEQSA
KKLIAQGVVDSEEVSKKIELESSSLF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=776846 PK654_RS02255 WP_271697451.1 448093..449133(+) (pilT) [Vibrio sp. SCSIO 43137]
ATGGATATCTCAGAGTTACTGGAATTTAGTGTAAAGCATAACGCATCAGATTTGCATCTTTCTGCAGGTGTTCCTCCTAT
GATACGTATTGACGGCGATGTTAGAAAGCTTGGTGTACCCGCTTTAGAGCATTCAGATGTGCATCGTATGGTTTTTGATA
TTATGACCGACGCGCAAAAGAGTGAGTTCGAAGGTAACCTAGAAGTGGACTTTTCTTTTGAACTTCCCCAGATTGGCCGT
TTTCGTGTTAATGCATTTAATCAGAGCCGGGGCTGCTCAGCTGTTTTCAGAACCATTCCAACTGATATTCCGACTCTTCA
GGAGCTTGAGTCTCCCGCCATATTTAATGACATTGCTGAGATGGAGAAAGGGCTGGTTCTGGTAACGGGGCCGACAGGTT
CAGGTAAGTCGACCACTCTTGCTGCTATGGTTGATCACGTTAACCAGAACTTTAATAAGCATATCCTGACTATCGAAGAT
CCCATTGAGTTTCTGCACAGCAATAAAAAATGCCTGATAAACCAGAGGGAAGTGCATAAGGATACTCATAGCTTTAAGGC
GGCGCTTCGTTCTGCCCTGCGTGAAGACCCGGATGTGATTCTGGTGGGAGAGTTAAGGGATCAGGAGACCATTAGTCTTG
CCTTGACTGCCGCTGAGACTGGTCATCTAGTATTTGGTACCTTGCATACCAGTTCAGCCGCTAAAACCATAGACAGAATC
ATCGATGTATTTCCCGGTAATGATAAAGCCATGGTACGTTCCATGTTGTCCGAGTCATTGCGGGCGGTGATTGCTCAGCG
TCTGCTTAAACGGGTTGGCGGCGGCCGTGTCGCCTGCCATGAGATTATGATGGCAACCCCTGCGATCAGAAACCTTATCC
GGGAAGATAAGGTGGCGCAGATGTACTCAATCATTCAGACCGGCGCTGCATCAGGCATGGTGACAATGGAGCAGAGTGCG
AAAAAACTGATTGCTCAGGGCGTGGTTGATTCAGAAGAAGTCAGTAAAAAAATTGAGCTGGAATCCAGTTCGCTGTTTTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.337

98.555

0.841
  pilT Vibrio cholerae strain A1552

85.337

98.555

0.841
  pilT Acinetobacter baumannii D1279779

70.482

95.954

0.676
  pilT Acinetobacter baumannii strain A118

70.482

95.954

0.676
  pilT Acinetobacter nosocomialis M2

70.181

95.954

0.673
  pilT Acinetobacter baylyi ADP1

69.394

95.376

0.662
  pilT Pseudomonas aeruginosa PAK

66.765

98.266

0.656
  pilT Pseudomonas stutzeri DSM 10701

67.062

97.399

0.653
  pilT Legionella pneumophila strain Lp02

67.781

95.087

0.645
  pilT Legionella pneumophila strain ERS1305867

67.781

95.087

0.645
  pilT Neisseria meningitidis 8013

61.96

100

0.621
  pilT Neisseria gonorrhoeae MS11

61.671

100

0.618
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

91.908

0.494
  pilU Vibrio cholerae strain A1552

41.538

93.931

0.39
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.532

0.387
  pilU Acinetobacter baylyi ADP1

39.875

92.775

0.37