Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PK654_RS02260 Genome accession   NZ_CP116383
Coordinates   449145..450269 (+) Length   374 a.a.
NCBI ID   WP_271697452.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43137     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444145..455269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PK654_RS02230 (PK654_02230) - 445005..445601 (-) 597 WP_271697446.1 XTP/dITP diphosphatase -
  PK654_RS02235 (PK654_02235) yggU 445635..445922 (-) 288 WP_271697447.1 DUF167 family protein YggU -
  PK654_RS02240 (PK654_02240) - 445922..446479 (-) 558 WP_271697448.1 YggT family protein -
  PK654_RS02245 (PK654_02245) proC 446516..447334 (-) 819 WP_271697449.1 pyrroline-5-carboxylate reductase -
  PK654_RS02250 (PK654_02250) - 447358..448068 (-) 711 WP_271697450.1 YggS family pyridoxal phosphate-dependent enzyme -
  PK654_RS02255 (PK654_02255) pilT 448093..449133 (+) 1041 WP_271697451.1 type IV pilus twitching motility protein PilT Machinery gene
  PK654_RS02260 (PK654_02260) pilU 449145..450269 (+) 1125 WP_271697452.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PK654_RS02265 (PK654_02265) ruvX 450291..450716 (-) 426 WP_271697453.1 Holliday junction resolvase RuvX -
  PK654_RS02270 (PK654_02270) - 450767..451330 (-) 564 WP_271697454.1 YqgE/AlgH family protein -
  PK654_RS02275 (PK654_02275) gshB 451389..452336 (-) 948 WP_271697455.1 glutathione synthase -
  PK654_RS02280 (PK654_02280) rsmE 452349..453080 (-) 732 WP_271697456.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PK654_RS02285 (PK654_02285) - 453217..453717 (-) 501 WP_271697457.1 SprT family zinc-dependent metalloprotease -
  PK654_RS02290 (PK654_02290) - 453785..454582 (-) 798 WP_271697458.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41205.88 Da        Isoelectric Point: 5.2822

>NTDB_id=776847 PK654_RS02260 WP_271697452.1 449145..450269(+) (pilU) [Vibrio sp. SCSIO 43137]
MTEMTSLLEAMLQQKASDMYLTVDAPCQLRVNGQLQPVGETLDKNTVASLLDTIMDSEQQQLYRESKEANFAIVQPGDGQ
NSARYRVSAFFQREQPGAVIRRIETQIPTFSELALPDVLKQLATEKRGLVLVVGATGSGKSTSMAAMTGYRNSHKSGHIL
TVEDPIEFVHQHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQA
LERILHLMPKDKREAFLFDLASNLKGIVAQQLVVDKNGQQRHGVFEILLNTPRVSDLIRRGELNELKATMAKSAGAGMIT
FDQSLFELVQQGKISEAEALHSADSANDLKLMIKSNGSTFSSSGSLDDVTIDMG

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=776847 PK654_RS02260 WP_271697452.1 449145..450269(+) (pilU) [Vibrio sp. SCSIO 43137]
ATGACAGAAATGACCAGTCTGCTGGAGGCAATGTTGCAACAGAAGGCATCGGATATGTACCTGACGGTCGATGCTCCCTG
TCAGCTAAGGGTTAACGGGCAGCTTCAGCCTGTGGGAGAAACGCTGGACAAGAATACGGTTGCTTCTCTGCTGGACACTA
TTATGGACAGCGAGCAACAACAGCTTTACCGGGAGAGTAAAGAAGCTAATTTTGCTATTGTTCAGCCGGGAGACGGGCAG
AACAGTGCCCGCTACCGGGTTAGTGCCTTTTTTCAGCGTGAGCAGCCGGGTGCCGTTATCCGCCGTATTGAAACGCAGAT
TCCGACTTTCTCTGAGCTTGCTTTGCCAGATGTACTGAAACAGCTGGCCACAGAAAAGCGCGGTCTGGTGCTGGTGGTTG
GTGCTACGGGGTCGGGTAAATCGACCTCTATGGCCGCAATGACAGGCTACCGGAATAGCCATAAATCAGGCCATATTCTT
ACCGTAGAAGATCCTATTGAGTTTGTTCATCAGCACGACAAATGCATAGTGACTCAGCGGGAAGTCGGGTTGGATACAGA
GAGTTATGAAGTCGCATTAAAGAACTCTCTCAGACAGGCACCGGATATGATTCTTATCGGTGAGATCCGCAGCAGGGAAA
CCATGGAGTACGCCATGACTTTCGCTGAGACCGGCCACCTCTGTATGGCGACGCTTCATGCGAACAATGCTAATCAGGCG
CTGGAGCGGATTCTGCACCTGATGCCTAAAGATAAAAGAGAAGCTTTTCTGTTTGATTTAGCCAGCAACCTTAAAGGGAT
TGTGGCTCAGCAACTTGTAGTTGATAAAAACGGACAGCAGCGTCATGGTGTTTTTGAAATTCTGCTAAATACACCAAGGG
TTTCTGACCTTATCCGTCGCGGTGAGTTAAACGAGCTTAAAGCAACTATGGCAAAATCGGCCGGGGCAGGGATGATCACT
TTTGATCAGTCGCTGTTTGAGTTGGTACAACAAGGCAAGATAAGCGAAGCCGAAGCGCTGCACAGTGCGGATTCAGCCAA
TGATCTTAAGTTGATGATCAAAAGTAACGGAAGTACGTTTTCTTCATCAGGTTCTCTTGATGATGTCACCATTGATATGG
GCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

70.62

99.198

0.701
  pilU Pseudomonas stutzeri DSM 10701

56.145

95.722

0.537
  pilU Acinetobacter baylyi ADP1

53.541

94.385

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.228

91.444

0.377
  pilT Legionella pneumophila strain Lp02

40.351

91.444

0.369
  pilT Legionella pneumophila strain ERS1305867

40.351

91.444

0.369
  pilT Acinetobacter baylyi ADP1

39.826

91.979

0.366
  pilT Acinetobacter baumannii strain A118

39.474

91.444

0.361
  pilT Acinetobacter nosocomialis M2

39.474

91.444

0.361
  pilT Acinetobacter baumannii D1279779

39.474

91.444

0.361