Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFX98_RS12530 Genome accession   NZ_CP116346
Coordinates   2702781..2703824 (+) Length   347 a.a.
NCBI ID   WP_285230841.1    Uniprot ID   A0AA95SNI7
Organism   Paucibacter sediminis strain S2-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2697781..2708824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFX98_RS12510 (PFX98_12510) - 2698672..2699889 (-) 1218 WP_285230837.1 cytochrome c -
  PFX98_RS12515 (PFX98_12515) - 2699886..2700572 (-) 687 WP_285230838.1 c-type cytochrome -
  PFX98_RS12520 (PFX98_12520) - 2700638..2702059 (-) 1422 WP_285230839.1 PLP-dependent aminotransferase family protein -
  PFX98_RS12525 (PFX98_12525) - 2702056..2702754 (-) 699 WP_285230840.1 YggS family pyridoxal phosphate-dependent enzyme -
  PFX98_RS12530 (PFX98_12530) pilT 2702781..2703824 (+) 1044 WP_285230841.1 type IV pilus twitching motility protein PilT Machinery gene
  PFX98_RS12535 (PFX98_12535) pilU 2703912..2705048 (+) 1137 WP_285230842.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PFX98_RS12540 (PFX98_12540) - 2705059..2705958 (+) 900 WP_285230843.1 NAD(P)-dependent oxidoreductase -
  PFX98_RS12545 (PFX98_12545) - 2705962..2706600 (-) 639 WP_285230844.1 BON domain-containing protein -
  PFX98_RS12550 (PFX98_12550) - 2706632..2707216 (-) 585 WP_285230845.1 SIS domain-containing protein -
  PFX98_RS12555 (PFX98_12555) - 2707247..2707630 (-) 384 WP_285230846.1 YraN family protein -
  PFX98_RS12560 (PFX98_12560) rsmI 2707623..2708540 (+) 918 WP_285230847.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38186.81 Da        Isoelectric Point: 7.0670

>NTDB_id=776792 PFX98_RS12530 WP_285230841.1 2702781..2703824(+) (pilT) [Paucibacter sediminis strain S2-9]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLEHRQVHDMVYDIMNDSQRKAYEETLEVDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNCPKVFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNESEYGHVLTIED
PIEFVHESKKCLVNQREVGPHTLSFANALRSALREDPDAVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRAMVSESLVAVISQSLCKLKDGSGRVAAHEIMLGTSAIRNLIRENKVAQMYSAIQTGNQQGMQTLDQN
LTDLVRRNVIAPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=776792 PFX98_RS12530 WP_285230841.1 2702781..2703824(+) (pilT) [Paucibacter sediminis strain S2-9]
ATGGACATTACCCAACTGCTGGCATTCTCGGTCAAGAACAAGGCCTCCGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGTGATGTGCGTCGCATCAATGTCGAGCCGCTCGAGCACCGCCAGGTGCACGACATGGTGTACGACA
TCATGAATGACTCGCAGCGCAAGGCCTATGAGGAAACGCTGGAGGTCGACTTCTCCTTCGAGATCCAGGGCCTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATCCCGTCCAAGATCCTCACGCTGGA
GCAGCTCAACTGCCCCAAGGTGTTTGCCGATCTGGCACTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGTT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTGAACCACCTGAACGAGAGCGAGTACGGCCACGTGCTGACGATCGAGGAC
CCGATCGAGTTCGTGCACGAATCAAAGAAGTGCTTGGTCAACCAGCGCGAGGTCGGCCCGCACACGCTGAGCTTCGCCAA
CGCGCTACGCTCGGCACTGCGCGAGGACCCGGATGCGGTGCTGGTGGGTGAAATGCGCGACCTCGAAACCATCCGCCTGG
CGCTCACCGCGGCCGAGACCGGCCACTTGGTGTTCGGCACCCTGCACACCAGTTCGGCCGCCAAGACGGTGGACCGTATC
GTCGACGTGTTCCCCGCGGCCGAGAAGGAAATGGTGCGCGCGATGGTGTCGGAATCGCTGGTGGCGGTGATCTCGCAAAG
CCTGTGCAAGCTCAAGGACGGCAGCGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACTTCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCACAGATGTACTCGGCCATCCAGACCGGCAACCAGCAGGGCATGCAGACGCTGGACCAGAAT
CTCACCGACCTGGTGCGGCGCAATGTGATCGCGCCCGCCGAGGCGCGCGCAAAAGCGAAGTTCCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

74.783

99.424

0.744
  pilT Acinetobacter baumannii D1279779

74.783

99.424

0.744
  pilT Acinetobacter baumannii strain A118

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.406

0.651
  pilT Vibrio cholerae strain A1552

66.864

97.406

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Acinetobacter baylyi ADP1

39.306

99.712

0.392