Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PFX98_RS12535 Genome accession   NZ_CP116346
Coordinates   2703912..2705048 (+) Length   378 a.a.
NCBI ID   WP_285230842.1    Uniprot ID   A0AA95SL39
Organism   Paucibacter sediminis strain S2-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2698912..2710048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFX98_RS12515 (PFX98_12515) - 2699886..2700572 (-) 687 WP_285230838.1 c-type cytochrome -
  PFX98_RS12520 (PFX98_12520) - 2700638..2702059 (-) 1422 WP_285230839.1 PLP-dependent aminotransferase family protein -
  PFX98_RS12525 (PFX98_12525) - 2702056..2702754 (-) 699 WP_285230840.1 YggS family pyridoxal phosphate-dependent enzyme -
  PFX98_RS12530 (PFX98_12530) pilT 2702781..2703824 (+) 1044 WP_285230841.1 type IV pilus twitching motility protein PilT Machinery gene
  PFX98_RS12535 (PFX98_12535) pilU 2703912..2705048 (+) 1137 WP_285230842.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PFX98_RS12540 (PFX98_12540) - 2705059..2705958 (+) 900 WP_285230843.1 NAD(P)-dependent oxidoreductase -
  PFX98_RS12545 (PFX98_12545) - 2705962..2706600 (-) 639 WP_285230844.1 BON domain-containing protein -
  PFX98_RS12550 (PFX98_12550) - 2706632..2707216 (-) 585 WP_285230845.1 SIS domain-containing protein -
  PFX98_RS12555 (PFX98_12555) - 2707247..2707630 (-) 384 WP_285230846.1 YraN family protein -
  PFX98_RS12560 (PFX98_12560) rsmI 2707623..2708540 (+) 918 WP_285230847.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  PFX98_RS12565 (PFX98_12565) - 2708558..2708887 (-) 330 WP_285230848.1 hypothetical protein -
  PFX98_RS12570 (PFX98_12570) - 2708971..2709945 (-) 975 WP_285230849.1 NAD(P)H-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42248.49 Da        Isoelectric Point: 6.8297

>NTDB_id=776793 PFX98_RS12535 WP_285230842.1 2703912..2705048(+) (pilU) [Paucibacter sediminis strain S2-9]
MERDQASKFINDLLRLMVSRKGSDLFLTADFPPAIKVDGKVTKVSPQPLTGQHTLQLARSIMNDKQAAEFERTKECNFAI
SPQGVGRFRCNAFMQQGHVGLVLRTIPASLPTIASLELPPILNEVVQTKRGLVIVVGATGSGKSTTLAAMIDHRNESTYG
HIITIEDPVEFVHPHKNCIVTQREVGIDTDNWEMALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSIISQRLLPRREGKGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKRSREQG
MQTFDQALFDLYEFGRVNYEDALRNADSVNDLRLQIKLNSERARSGDLSSGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=776793 PFX98_RS12535 WP_285230842.1 2703912..2705048(+) (pilU) [Paucibacter sediminis strain S2-9]
ATGGAACGCGATCAGGCGTCAAAGTTCATCAATGATCTGCTGCGCCTGATGGTGTCGCGCAAGGGCTCGGATCTGTTCCT
CACCGCCGACTTCCCGCCCGCCATCAAGGTGGATGGCAAGGTCACCAAGGTCTCGCCGCAGCCGCTCACCGGCCAGCACA
CCCTGCAGCTGGCGCGTTCCATCATGAACGACAAGCAGGCGGCCGAGTTCGAGCGCACCAAGGAATGCAATTTCGCCATC
TCGCCGCAGGGCGTGGGGCGCTTCCGCTGCAATGCCTTCATGCAGCAGGGCCATGTGGGCCTGGTGCTGCGCACCATCCC
GGCCTCGCTGCCGACCATCGCCTCGCTGGAGCTGCCGCCCATCCTCAACGAGGTGGTGCAGACCAAGCGCGGCCTGGTGA
TCGTGGTGGGCGCCACCGGCTCGGGCAAGAGCACCACGCTGGCGGCGATGATCGACCATCGCAACGAGAGCACCTACGGC
CACATCATCACGATCGAGGACCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTGGGCAT
CGACACCGACAATTGGGAGATGGCGCTGAAAAACACGCTGCGGCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTGGCCTTTGCCGAGACCGGCCACCTCTGCATGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTGATGGACCTGTCGCTGAATCT
GCGCTCCATCATCTCGCAGCGCCTGCTGCCGCGCCGCGAGGGCAAGGGCCGCGTCGCCGCGGTGGAGATCCTGCTCAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGTGAGATCAAGGAGATCATGAAGCGCTCGCGCGAGCAGGGC
ATGCAGACCTTTGACCAGGCCCTGTTCGATCTCTACGAGTTCGGCCGCGTCAACTACGAAGACGCGCTGCGCAACGCCGA
CTCGGTGAACGATCTGCGCCTGCAGATCAAGCTGAACAGCGAACGCGCGCGCAGCGGCGACCTCTCATCCGGCACCGAGC
ACCTGACGATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.022

96.825

0.601
  pilU Acinetobacter baylyi ADP1

58.774

94.974

0.558
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.884

89.153

0.418
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baylyi ADP1

43.953

89.683

0.394
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.976

87.831

0.386
  pilT Vibrio cholerae strain A1552

43.976

87.831

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain ERS1305867

45.031

85.185

0.384
  pilT Legionella pneumophila strain Lp02

45.031

85.185

0.384