Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PJ259_RS19480 Genome accession   NZ_CP116340
Coordinates   4197093..4198127 (+) Length   344 a.a.
NCBI ID   WP_007161666.1    Uniprot ID   -
Organism   Pseudomonas sp. JBR1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192093..4203127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ259_RS19460 (PJ259_19460) - 4192749..4194707 (-) 1959 WP_271653865.1 dynamin family protein -
  PJ259_RS19465 (PJ259_19465) - 4194837..4195430 (-) 594 WP_271653866.1 YggT family protein -
  PJ259_RS19470 (PJ259_19470) proC 4195440..4196258 (-) 819 WP_271653867.1 pyrroline-5-carboxylate reductase -
  PJ259_RS19475 (PJ259_19475) - 4196268..4196966 (-) 699 WP_271653868.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJ259_RS19480 (PJ259_19480) pilT 4197093..4198127 (+) 1035 WP_007161666.1 type IV pilus twitching motility protein PilT Machinery gene
  PJ259_RS19485 (PJ259_19485) pilU 4198127..4199269 (+) 1143 WP_059313545.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJ259_RS19490 (PJ259_19490) - 4199266..4200708 (-) 1443 WP_271653869.1 UdgX family uracil-DNA binding protein -
  PJ259_RS19495 (PJ259_19495) - 4200709..4201920 (-) 1212 WP_017640984.1 putative DNA modification/repair radical SAM protein -
  PJ259_RS19500 (PJ259_19500) - 4202053..4202607 (-) 555 WP_059313547.1 C40 family peptidase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37941.73 Da        Isoelectric Point: 6.8363

>NTDB_id=776694 PJ259_RS19480 WP_007161666.1 4197093..4198127(+) (pilT) [Pseudomonas sp. JBR1]
MDITELLAFSARQGASDLHLSAGLPPMIRVDGDVRRINLPAFEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGGLGMQTLDMCL
KALLAKGLISRDAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=776694 PJ259_RS19480 WP_007161666.1 4197093..4198127(+) (pilT) [Pseudomonas sp. JBR1]
ATGGATATCACCGAATTGCTGGCCTTTTCCGCGCGGCAAGGCGCCTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGCATCAATCTGCCGGCCTTCGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAATCAGAATCGTGGCGCTGGCGCGGTATTCCGGACCATCCCTTCCAAGGTGCTGAGCATGGA
AGAGCTGGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGACCGACCGGTT
CGGGCAAGTCCACCACCCTCGCGGCCATGCTCGACTACCTCAACAACACCAAGTACCACCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTCCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTGGGCTTTTCCGA
AGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCGGCCGAGACCGGGCACCTGGTGTTCGGCACCCTGCACACGACCTCGGCGGCCAAGACCATCGATCGGGTG
GTGGACGTCTTTCCGGCGGAAGAGAAATCCATGGTGCGTTCCATGTTGTCCGAATCCCTGCAGGCGGTGGTCTCCCAGAC
CCTGCTCAAGAAGGTCGGCGGCGGTCGGGTAGCCGCTCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTGGCGGCCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGCGCTCCTGGCCAAGGGGCTGATCAGCCGCGACGCGGCCCGCGAGAAGGCCAAGACGCCGGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.64

100

0.956
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39