Detailed information NTG0776695

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PJ259_RS19485
• Genome accession: NZ_CP116340
• Coordinates: 4198127..4199269 (+)
• Length: 380 a.a.
• NCBI ID: WP_059313545.1
• Uniprot ID: A0AAX3KVS0
• Organism: Pseudomonas sp. JBR1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4193127..4204269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PJ259_RS19465 (PJ259_19465)	-	4194837..4195430 (-)	594	WP_271653866.1	YggT family protein	-
PJ259_RS19470 (PJ259_19470)	proC	4195440..4196258 (-)	819	WP_271653867.1	pyrroline-5-carboxylate reductase	-
PJ259_RS19475 (PJ259_19475)	-	4196268..4196966 (-)	699	WP_271653868.1	YggS family pyridoxal phosphate-dependent enzyme	-
PJ259_RS19480 (PJ259_19480)	pilT	4197093..4198127 (+)	1035	WP_007161666.1	type IV pilus twitching motility protein PilT	Machinery gene
PJ259_RS19485 (PJ259_19485)	pilU	4198127..4199269 (+)	1143	WP_059313545.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PJ259_RS19490 (PJ259_19490)	-	4199266..4200708 (-)	1443	WP_271653869.1	UdgX family uracil-DNA binding protein	-
PJ259_RS19495 (PJ259_19495)	-	4200709..4201920 (-)	1212	WP_017640984.1	putative DNA modification/repair radical SAM protein	-
PJ259_RS19500 (PJ259_19500)	-	4202053..4202607 (-)	555	WP_059313547.1	C40 family peptidase	-
PJ259_RS19505 (PJ259_19505)	-	4203042..4203446 (+)	405	WP_059313548.1	TM2 domain-containing protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42459.77 Da
• Isoelectric Point: 7.3063

>NTDB_id=776695 PJ259_RS19485 WP_059313545.1 4198127..4199269(+) (pilU) [Pseudomonas sp. JBR1]
MDFDRFLQLMVDKGASDLFITTGVPPSMKINGRMTPVTKDSLSPEQCRALVLGVMTDEQRQEFQQKRECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIEVNIPTVEQLRLPEIIKKLAMTKRGLVIFVGATGTGKSTSLAAMIGHRNKNSAGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEIRSRDTMEHALAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQTWMDLSLNLKGIVAQQLVPTIDGQGRRAVIEVLLNTPLATDLIRKGEVHELKALMKKSGELGMQTFDQS
LYSLYRDGEISYEAALAHADSANDLRLMIKLGSETDADHLNQTTRNLSVDMGDDPPRRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=776695 PJ259_RS19485 WP_059313545.1 4198127..4199269(+) (pilU) [Pseudomonas sp. JBR1]
ATGGATTTCGACAGGTTCCTGCAGCTGATGGTGGACAAGGGGGCGTCCGACCTCTTCATCACCACCGGCGTGCCCCCTTC
GATGAAGATCAATGGGCGCATGACGCCGGTCACCAAGGATTCGCTGTCTCCCGAGCAGTGCCGGGCCCTGGTGCTGGGGG
TGATGACCGACGAACAGCGGCAGGAATTCCAGCAGAAGCGCGAGTGCAACTTCGCCATCAGCGCCCGGGGCATTGGTCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAATCTGGTGGGCATGGTGCTGCGCCGCATCGAGGTCAACATTCCCACGGTCGA
GCAACTGCGCCTGCCGGAGATCATCAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCCATCGCAACAAGAACTCGGCGGGGCACATCATCTCCATCGAGGAT
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAGGTAGGGATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCATGATCGGCGAGATCCGCAGTCGGGACACCATGGAGCATG
CCCTGGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCCACTTCTTTCCGGCCGATCGACACAGCCAGACCTGGATGGACCTGTCGCTCAACCTCAAGGGCATCGTCGCCCAGCA
GTTGGTGCCGACCATCGATGGTCAGGGGCGCCGCGCGGTGATCGAGGTGCTGCTCAACACCCCGCTGGCCACCGACCTGA
TCCGCAAGGGCGAGGTGCACGAACTCAAGGCGCTGATGAAGAAGTCCGGCGAACTGGGCATGCAGACCTTCGACCAGTCG
CTCTACAGCCTCTATCGCGACGGCGAGATCAGCTACGAAGCGGCCCTGGCCCACGCCGATTCCGCCAACGACCTGCGGCT
GATGATCAAGCTGGGGTCGGAGACAGATGCCGATCACCTCAATCAGACCACCCGCAATCTCTCGGTGGACATGGGCGACG
ACCCACCCAGGCGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.428	100	0.776
pilU	Acinetobacter baylyi ADP1	65.826	93.947	0.618
pilU	Vibrio cholerae strain A1552	57.143	97.632	0.558
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	89.211	0.374