Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PID00_RS12535 Genome accession   NZ_CP116067
Coordinates   2475348..2476328 (-) Length   326 a.a.
NCBI ID   WP_088144374.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-S792     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2470348..2481328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PID00_RS12505 (PID00_12505) yggI 2471013..2471510 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  PID00_RS12510 (PID00_12510) endA 2471605..2472312 (+) 708 WP_000286497.1 deoxyribonuclease I -
  PID00_RS12515 (PID00_12515) rsmE 2472392..2473123 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PID00_RS12520 (PID00_12520) gshB 2473136..2474086 (+) 951 WP_000593273.1 glutathione synthase -
  PID00_RS12525 (PID00_12525) yqgE 2474195..2474758 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  PID00_RS12530 (PID00_12530) ruvX 2474758..2475174 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  PID00_RS12535 (PID00_12535) pilT 2475348..2476328 (-) 981 WP_088144374.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PID00_RS12540 (PID00_12540) yggS 2476346..2477050 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  PID00_RS12545 (PID00_12545) yggT 2477068..2477634 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  PID00_RS12550 (PID00_12550) yggU 2477631..2477921 (+) 291 WP_001277194.1 DUF167 family protein YggU -
  PID00_RS12555 (PID00_12555) rdgB 2477929..2478522 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  PID00_RS12560 (PID00_12560) hemW 2478515..2479651 (+) 1137 WP_096121743.1 radical SAM family heme chaperone HemW -
  PID00_RS12565 (PID00_12565) yggM 2479716..2480723 (-) 1008 WP_001562053.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.22 Da        Isoelectric Point: 5.7980

>NTDB_id=775600 PID00_RS12535 WP_088144374.1 2475348..2476328(-) (pilT) [Escherichia coli strain DETEC-S792]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=775600 PID00_RS12535 WP_088144374.1 2475348..2476328(-) (pilT) [Escherichia coli strain DETEC-S792]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365