Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R74_RS18090 Genome accession   NZ_AP022281
Coordinates   3955739..3956773 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain WP8-W19-CRE-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3950739..3961773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R74_RS18060 (WP8W19C03_35080) glnK 3951320..3951658 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  H7R74_RS18065 (WP8W19C03_35090) arfB 3951860..3952276 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  H7R74_RS18070 (WP8W19C03_35100) - 3952309..3952800 (+) 492 WP_064338817.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  H7R74_RS18075 (WP8W19C03_35110) - 3953160..3953585 (-) 426 WP_040098961.1 hypothetical protein -
  H7R74_RS18080 (WP8W19C03_35120) yaaA 3953780..3954553 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  H7R74_RS18085 (WP8W19C03_35130) pilU 3954601..3955710 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  H7R74_RS18090 (WP8W19C03_35140) pilT 3955739..3956773 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  H7R74_RS18095 (WP8W19C03_35150) - 3956813..3957514 (+) 702 WP_058057968.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R74_RS18100 (WP8W19C03_35160) proC 3957657..3958481 (+) 825 WP_064338819.1 pyrroline-5-carboxylate reductase -
  H7R74_RS18105 (WP8W19C03_35170) - 3958493..3959044 (+) 552 WP_005340343.1 YggT family protein -
  H7R74_RS18110 (WP8W19C03_35180) yggU 3959044..3959346 (+) 303 WP_064338820.1 DUF167 family protein YggU -
  H7R74_RS18115 (WP8W19C03_35190) - 3959390..3959809 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  H7R74_RS18120 (WP8W19C03_35200) - 3959911..3961107 (-) 1197 WP_064338821.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=77520 H7R74_RS18090 WP_005340354.1 3955739..3956773(-) (pilT) [Aeromonas veronii strain WP8-W19-CRE-03]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=77520 H7R74_RS18090 WP_005340354.1 3955739..3956773(-) (pilT) [Aeromonas veronii strain WP8-W19-CRE-03]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTACCCAATCTGGCCCGT
TTTCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGTT
CGGGTAAGTCGACCACCCTGGCGGCCATGGTCAATTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAGGAC
CCTATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTTCACCGGGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACGGCCGCCGAAACCGGCCATCTGGTGTTTGGCACTCTGCACACTTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCGGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCAGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment