Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R74_RS18085 Genome accession   NZ_AP022281
Coordinates   3954601..3955710 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain WP8-W19-CRE-03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3949601..3960710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R74_RS18055 (WP8W19C03_35070) - 3950054..3951061 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  H7R74_RS18060 (WP8W19C03_35080) glnK 3951320..3951658 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  H7R74_RS18065 (WP8W19C03_35090) arfB 3951860..3952276 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  H7R74_RS18070 (WP8W19C03_35100) - 3952309..3952800 (+) 492 WP_064338817.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  H7R74_RS18075 (WP8W19C03_35110) - 3953160..3953585 (-) 426 WP_040098961.1 hypothetical protein -
  H7R74_RS18080 (WP8W19C03_35120) yaaA 3953780..3954553 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  H7R74_RS18085 (WP8W19C03_35130) pilU 3954601..3955710 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  H7R74_RS18090 (WP8W19C03_35140) pilT 3955739..3956773 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  H7R74_RS18095 (WP8W19C03_35150) - 3956813..3957514 (+) 702 WP_058057968.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R74_RS18100 (WP8W19C03_35160) proC 3957657..3958481 (+) 825 WP_064338819.1 pyrroline-5-carboxylate reductase -
  H7R74_RS18105 (WP8W19C03_35170) - 3958493..3959044 (+) 552 WP_005340343.1 YggT family protein -
  H7R74_RS18110 (WP8W19C03_35180) yggU 3959044..3959346 (+) 303 WP_064338820.1 DUF167 family protein YggU -
  H7R74_RS18115 (WP8W19C03_35190) - 3959390..3959809 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=77519 H7R74_RS18085 WP_005340356.1 3954601..3955710(-) (pilU) [Aeromonas veronii strain WP8-W19-CRE-03]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=77519 H7R74_RS18085 WP_005340356.1 3954601..3955710(-) (pilU) [Aeromonas veronii strain WP8-W19-CRE-03]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCAGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGTATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAAATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTTGGCGCCACCGGGG
CGGGTAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGGAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACTGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTTGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment