Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PE064_RS11010 Genome accession   NZ_CP116007
Coordinates   2366023..2367060 (+) Length   345 a.a.
NCBI ID   WP_093490243.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain DMF-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2361023..2372060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE064_RS10980 (PE064_10980) pdxH 2361835..2362437 (+) 603 WP_271249231.1 pyridoxamine 5'-phosphate oxidase -
  PE064_RS10985 (PE064_10985) aac(6') 2362488..2362883 (+) 396 WP_271251520.1 aminoglycoside 6'-N-acetyltransferase -
  PE064_RS10990 (PE064_10990) - 2362887..2363681 (+) 795 WP_271249232.1 ABC transporter substrate-binding protein -
  PE064_RS10995 (PE064_10995) - 2363848..2364312 (-) 465 WP_271249233.1 DUF4426 domain-containing protein -
  PE064_RS11000 (PE064_11000) proC 2364373..2365215 (-) 843 WP_271249234.1 pyrroline-5-carboxylate reductase -
  PE064_RS11005 (PE064_11005) - 2365246..2365929 (-) 684 WP_271251521.1 YggS family pyridoxal phosphate-dependent enzyme -
  PE064_RS11010 (PE064_11010) pilT 2366023..2367060 (+) 1038 WP_093490243.1 type IV pilus twitching motility protein PilT Machinery gene
  PE064_RS11015 (PE064_11015) pilU 2367192..2368322 (+) 1131 WP_162110440.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PE064_RS11020 (PE064_11020) - 2368504..2369013 (+) 510 WP_271249235.1 DUF6622 family protein -
  PE064_RS11025 (PE064_11025) - 2368946..2370028 (-) 1083 WP_271249236.1 prolyl oligopeptidase family serine peptidase -
  PE064_RS11030 (PE064_11030) - 2370084..2370305 (-) 222 WP_162110444.1 helix-turn-helix transcriptional regulator -
  PE064_RS11035 (PE064_11035) - 2370307..2370819 (-) 513 WP_271249237.1 DUF2975 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38560.31 Da        Isoelectric Point: 6.7271

>NTDB_id=774883 PE064_RS11010 WP_093490243.1 2366023..2367060(+) (pilT) [Pseudoxanthomonas mexicana strain DMF-5]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
IDVFPAGEKPMVRSMLSESLRAVISQALLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMMTLDQHL
QDLVKRALITRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=774883 PE064_RS11010 WP_093490243.1 2366023..2367060(+) (pilT) [Pseudoxanthomonas mexicana strain DMF-5]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTAAAGAACAAGGCCTCGGACCTCCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCTGGCGCCGTCTTCCGTACCATTCCGTCGGAAGTGCTGACCCTGGA
AGATCTGGGCTGCCCGCCGCTGTTCCGCGAACTGATCCAGCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGGGCACATCCTCAGCGTCGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGTGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCGTG
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGATCGGCGGCGGCCGTACCGCGGCGTGGGAAATCATGGTGGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGACAGCAGAGCGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTGAAGCGCGCGCTGATCACCCGCCAGCAGGCCAAGGAATACGCGAAGGACAAGCGTCTCTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Acinetobacter baylyi ADP1

76.163

99.71

0.759
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.06

97.101

0.661
  pilT Vibrio cholerae strain A1552

68.06

97.101

0.661
  pilT Neisseria meningitidis 8013

64.223

98.841

0.635
  pilT Neisseria gonorrhoeae MS11

63.93

98.841

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397