Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PE064_RS11015 Genome accession   NZ_CP116007
Coordinates   2367192..2368322 (+) Length   376 a.a.
NCBI ID   WP_162110440.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain DMF-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2362192..2373322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE064_RS10985 (PE064_10985) aac(6') 2362488..2362883 (+) 396 WP_271251520.1 aminoglycoside 6'-N-acetyltransferase -
  PE064_RS10990 (PE064_10990) - 2362887..2363681 (+) 795 WP_271249232.1 ABC transporter substrate-binding protein -
  PE064_RS10995 (PE064_10995) - 2363848..2364312 (-) 465 WP_271249233.1 DUF4426 domain-containing protein -
  PE064_RS11000 (PE064_11000) proC 2364373..2365215 (-) 843 WP_271249234.1 pyrroline-5-carboxylate reductase -
  PE064_RS11005 (PE064_11005) - 2365246..2365929 (-) 684 WP_271251521.1 YggS family pyridoxal phosphate-dependent enzyme -
  PE064_RS11010 (PE064_11010) pilT 2366023..2367060 (+) 1038 WP_093490243.1 type IV pilus twitching motility protein PilT Machinery gene
  PE064_RS11015 (PE064_11015) pilU 2367192..2368322 (+) 1131 WP_162110440.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PE064_RS11020 (PE064_11020) - 2368504..2369013 (+) 510 WP_271249235.1 DUF6622 family protein -
  PE064_RS11025 (PE064_11025) - 2368946..2370028 (-) 1083 WP_271249236.1 prolyl oligopeptidase family serine peptidase -
  PE064_RS11030 (PE064_11030) - 2370084..2370305 (-) 222 WP_162110444.1 helix-turn-helix transcriptional regulator -
  PE064_RS11035 (PE064_11035) - 2370307..2370819 (-) 513 WP_271249237.1 DUF2975 domain-containing protein -
  PE064_RS11040 (PE064_11040) - 2371004..2372518 (-) 1515 WP_271249238.1 acetyl-CoA hydrolase/transferase family protein -
  PE064_RS11045 (PE064_11045) - 2372615..2373160 (-) 546 WP_271249239.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41893.23 Da        Isoelectric Point: 6.8286

>NTDB_id=774884 PE064_RS11015 WP_162110440.1 2367192..2368322(+) (pilU) [Pseudoxanthomonas mexicana strain DMF-5]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=774884 PE064_RS11015 WP_162110440.1 2367192..2368322(+) (pilU) [Pseudoxanthomonas mexicana strain DMF-5]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCGTCCATCAAGGTGCACGGCAAGATCTCGCCGATCACGCAGACGCCGCTGACGGCGCAGCAGTCGCGCGACCTGG
TGCTGAACGTGATGACGCCCTCGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGCATCGAGACGCGCATCCC
GACCATCGAAGAGCTGAACCTGCCGCCGGTCATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTGGGCG
CCACGGGTACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAAGACCCCATCGAGTTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGTGAAGTGGGCATCGACACCGACAG
CTGGGATGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGTA
TGGACCACGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACCCAGCTGCTGATGGACCTGTCGCTCAACCTGAAGGGCGTGGT
GGCGCAGCAGCTGATCCCCACGCCGGACGGCAAGGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCGCAGGGCGGCGACGCCCGCACGCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCGG
AAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.143

93.085

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362