Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PG907_RS13930 Genome accession   NZ_CP115955
Coordinates   2963965..2965008 (-) Length   347 a.a.
NCBI ID   WP_193027655.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2958965..2970008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG907_RS13900 - 2959494..2960030 (-) 537 WP_193027660.1 PilX N-terminal domain-containing pilus assembly protein -
  PG907_RS13905 - 2960034..2961062 (-) 1029 WP_275760337.1 PilW family protein -
  PG907_RS13910 pilV 2961059..2961649 (-) 591 WP_043899105.1 type IV pilus modification protein PilV -
  PG907_RS13915 - 2961646..2962134 (-) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  PG907_RS13920 - 2962138..2962578 (-) 441 WP_275760338.1 type IV pilin protein -
  PG907_RS13925 pilU 2962773..2963912 (-) 1140 WP_197386915.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG907_RS13930 pilT 2963965..2965008 (-) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  PG907_RS13935 - 2965133..2965855 (+) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG907_RS13940 proC 2965890..2966714 (+) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  PG907_RS13945 - 2966737..2967015 (+) 279 WP_193027652.1 hypothetical protein -
  PG907_RS13950 ubiA 2967012..2967887 (-) 876 WP_275760339.1 4-hydroxybenzoate octaprenyltransferase -
  PG907_RS13955 - 2967961..2968461 (-) 501 WP_003271865.1 Dps family protein -
  PG907_RS13960 - 2968621..2968863 (-) 243 WP_197386912.1 hypothetical protein -
  PG907_RS13965 - 2968922..2969125 (-) 204 WP_275760340.1 hypothetical protein -
  PG907_RS13970 - 2969620..2969811 (+) 192 WP_193027649.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38257.82 Da        Isoelectric Point: 6.8705

>NTDB_id=774608 PG907_RS13930 WP_193027655.1 2963965..2965008(-) (pilT) [Ralstonia pseudosolanacearum strain RUN2340]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLTLEDLKAPAVFSDLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=774608 PG907_RS13930 WP_193027655.1 2963965..2965008(-) (pilT) [Ralstonia pseudosolanacearum strain RUN2340]
ATGGACATCGCGCAGCTTCTGGCCTTCTCTGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCTCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACTCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCGGACTTCTCGTTCGAGATTCCCGGTCTGTCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCTTCCGCCGTGTTCCGGACGATTCCGTCCAAGGTGCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCTCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACCGCAACGAAAACGACCTGGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAATCAGCGCGAACTGGGGCCGCATACCCATTCGTTCGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCTGATGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAAGCGGTGATTTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACACTGGATCAGTGC
CTGTCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

99.424

0.718
  pilT Acinetobacter baumannii D1279779

72.174

99.424

0.718
  pilT Acinetobacter baumannii strain A118

72.174

99.424

0.718
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Neisseria meningitidis 8013

68.208

99.712

0.68
  pilT Legionella pneumophila strain Lp02

67.536

99.424

0.671
  pilT Legionella pneumophila strain ERS1305867

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.118

0.651
  pilT Vibrio cholerae strain A1552

67.062

97.118

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401