Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG907_RS13925 Genome accession   NZ_CP115955
Coordinates   2962773..2963912 (-) Length   379 a.a.
NCBI ID   WP_197386915.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2957773..2968912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG907_RS13900 - 2959494..2960030 (-) 537 WP_193027660.1 PilX N-terminal domain-containing pilus assembly protein -
  PG907_RS13905 - 2960034..2961062 (-) 1029 WP_275760337.1 PilW family protein -
  PG907_RS13910 pilV 2961059..2961649 (-) 591 WP_043899105.1 type IV pilus modification protein PilV -
  PG907_RS13915 - 2961646..2962134 (-) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  PG907_RS13920 - 2962138..2962578 (-) 441 WP_275760338.1 type IV pilin protein -
  PG907_RS13925 pilU 2962773..2963912 (-) 1140 WP_197386915.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG907_RS13930 pilT 2963965..2965008 (-) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  PG907_RS13935 - 2965133..2965855 (+) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG907_RS13940 proC 2965890..2966714 (+) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  PG907_RS13945 - 2966737..2967015 (+) 279 WP_193027652.1 hypothetical protein -
  PG907_RS13950 ubiA 2967012..2967887 (-) 876 WP_275760339.1 4-hydroxybenzoate octaprenyltransferase -
  PG907_RS13955 - 2967961..2968461 (-) 501 WP_003271865.1 Dps family protein -
  PG907_RS13960 - 2968621..2968863 (-) 243 WP_197386912.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42251.81 Da        Isoelectric Point: 6.9274

>NTDB_id=774607 PG907_RS13925 WP_197386915.1 2962773..2963912(-) (pilU) [Ralstonia pseudosolanacearum strain RUN2340]
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPATGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEIHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=774607 PG907_RS13925 WP_197386915.1 2962773..2963912(-) (pilU) [Ralstonia pseudosolanacearum strain RUN2340]
ATGCTGGACCGCGACGCCGCCACCAGATATATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACCGCAGAATTTCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGCTGACCACCGTCC
AGGCCATGGGCCTGGTCAAGGCGATCATGAGCGAGAAGCAGTTGCGCGAGTTCGAAGAGTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCGCCACCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CACCACCAAGATCCCGACGCTCGCCGAACTGGATCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTGGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGTTACCGCAATGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATACGTGCACGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
CGTGGACACGGAATCCTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGTCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGACCTGTCCCTGAA
CTTGCGCGCCATGATCGCGCAGCGTCTGCTGCCGCGCAAAGGCAAGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGATACACGAGCTCAAGGAGGTCATGAAGAAGTCGCGCGAGCAG
GGCATGGTCTGTTTCGACCAGGCGCTGTTCGAGCTCTACGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCACAGCAAGCGCGGCGGGCCGGTGGATCTGACGGCTGGCACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.04

93.14

0.578
  pilU Acinetobacter baylyi ADP1

55.71

94.723

0.528
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.844

87.863

0.385
  pilT Legionella pneumophila strain ERS1305867

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

42.433

88.918

0.377
  pilT Vibrio cholerae strain A1552

45.11

83.641

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.11

83.641

0.377
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.246

88.918

0.367
  pilT Acinetobacter nosocomialis M2

43.26

84.169

0.364
  pilT Acinetobacter baumannii D1279779

43.26

84.169

0.364
  pilT Acinetobacter baumannii strain A118

43.26

84.169

0.364