Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PAK23_RS18775 Genome accession   NZ_CP115817
Coordinates   3990544..3991578 (-) Length   344 a.a.
NCBI ID   WP_004373763.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain GD22SC3150TT     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3985544..3996578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAK23_RS18755 - 3986705..3987103 (-) 399 WP_264147243.1 TM2 domain-containing protein -
  PAK23_RS18760 - 3987252..3988463 (+) 1212 WP_276487659.1 putative DNA modification/repair radical SAM protein -
  PAK23_RS18765 - 3988463..3989278 (+) 816 WP_276487660.1 TIGR03915 family putative DNA repair protein -
  PAK23_RS18770 pilU 3989311..3990456 (-) 1146 WP_264147240.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PAK23_RS18775 pilT 3990544..3991578 (-) 1035 WP_004373763.1 type IV pilus twitching motility protein PilT Machinery gene
  PAK23_RS18780 - 3991654..3992346 (+) 693 WP_276487661.1 YggS family pyridoxal phosphate-dependent enzyme -
  PAK23_RS18785 proC 3992362..3993180 (+) 819 WP_276487662.1 pyrroline-5-carboxylate reductase -
  PAK23_RS18790 - 3993191..3993784 (+) 594 WP_264147237.1 YggT family protein -
  PAK23_RS18795 - 3993787..3994077 (+) 291 WP_276487663.1 DUF167 domain-containing protein -
  PAK23_RS18800 - 3994260..3996236 (+) 1977 WP_276487664.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37949.62 Da        Isoelectric Point: 6.8889

>NTDB_id=773824 PAK23_RS18775 WP_004373763.1 3990544..3991578(-) (pilT) [Pseudomonas mendocina strain GD22SC3150TT]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=773824 PAK23_RS18775 WP_004373763.1 3990544..3991578(-) (pilT) [Pseudomonas mendocina strain GD22SC3150TT]
ATGGATATCACTGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCGGGGCTACCGCCGAT
GATCCGCGTTGATGGCGATGTGCGTCGCATCAACCTGCCACCGCTGGATCACAAGCAGGTACACGCGCTGATTTACGACA
TCATGAATGACAAGCAGCGCAAGGATTACGAGGAGTTCCTCGAAACCGACTTCTCCTTCGAAGTTCCGGGCGTGGCGCGC
TTCCGGGTCAACGCATTCAACCAGAACCGTGGCGCCGGTGCGGTATTCCGTACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTGGGCATGGGCGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTTACCGGTCCTACCGGTT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTACCACCACATCCTCACCATTGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGTCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTGCGTTCGATGCTGTCGGAATCCTTGCAGGCGGTAATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGTGTTGCCGCTCACGAGATCATGATCGGTACACCTGCGATTCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGTGGTGCGCTGGGCATGCAGACTCTGGATGCCTGCCTC
AAGACCCTGGTATCCAAGGGACTGGTCAGCCGCGACAGCGCGCGCGAGAAGGCCAAGAGCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395