Detailed information NTG0773823

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PAK23_RS18770
• Genome accession: NZ_CP115817
• Coordinates: 3989311..3990456 (-)
• Length: 381 a.a.
• NCBI ID: WP_264147240.1
• Uniprot ID: -
• Organism: Pseudomonas mendocina strain GD22SC3150TT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3984311..3995456

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAK23_RS18750	ntrC	3985166..3986599 (+)	1434	WP_264147244.1	nitrogen regulation protein NR(I)	-
PAK23_RS18755	-	3986705..3987103 (-)	399	WP_264147243.1	TM2 domain-containing protein	-
PAK23_RS18760	-	3987252..3988463 (+)	1212	WP_276487659.1	putative DNA modification/repair radical SAM protein	-
PAK23_RS18765	-	3988463..3989278 (+)	816	WP_276487660.1	TIGR03915 family putative DNA repair protein	-
PAK23_RS18770	pilU	3989311..3990456 (-)	1146	WP_264147240.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PAK23_RS18775	pilT	3990544..3991578 (-)	1035	WP_004373763.1	type IV pilus twitching motility protein PilT	Machinery gene
PAK23_RS18780	-	3991654..3992346 (+)	693	WP_276487661.1	YggS family pyridoxal phosphate-dependent enzyme	-
PAK23_RS18785	proC	3992362..3993180 (+)	819	WP_276487662.1	pyrroline-5-carboxylate reductase	-
PAK23_RS18790	-	3993191..3993784 (+)	594	WP_264147237.1	YggT family protein	-
PAK23_RS18795	-	3993787..3994077 (+)	291	WP_276487663.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42487.88 Da
• Isoelectric Point: 6.8333

>NTDB_id=773823 PAK23_RS18770 WP_264147240.1 3989311..3990456(-) (pilU) [Pseudomonas mendocina strain GD22SC3150TT]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEMTRETVLGVMNEAQRREFAEKHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKHSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=773823 PAK23_RS18770 WP_264147240.1 3989311..3990456(-) (pilU) [Pseudomonas mendocina strain GD22SC3150TT]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTACCGCCATC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCGCCGGAGATGACCCGCGAAACCGTGCTGGGTG
TGATGAACGAGGCGCAGCGCCGCGAGTTTGCCGAAAAGCACGAATGCAACTTCGCCATCAGCGCCCGCGGCGTCGGCCGT
TTCCGTGTCAGCGCCTTCTATCAGCGCAACCTGGTGGGCATGGTACTGCGTCGCATCGAGACCAACATTCCCACCATCGA
TGATCTGAAACTGCCGGAAATTCTCAAGAAGCTGGCGCTGACCAAACGGGGTCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGCACAGCAGCGGTCACATCATCTCCATCGAAGAC
CCCATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGGGAAGTGGGCATCGACACCGAGTCGTTCGAGGT
GGCGTTGAAGAACACCCTGCGTCAGGCGCCGGACGTAATCCTCATCGGTGAGGTGCGTACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCGGCCGACCGGCAGAATCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTACCGACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGTTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCT
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAAGACGCGCTGCTCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGCGATCACCTCACCAGCATGGCCCAGGGCCTGTCGCTGGAAGTCAGCGAGG
AAGATCCGGGGCGTCGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.388	100	0.924
pilU	Acinetobacter baylyi ADP1	64.986	93.701	0.609
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.617	87.664	0.365
pilT	Vibrio cholerae strain A1552	41.617	87.664	0.365