Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R41_RS13280 Genome accession   NZ_AP022238
Coordinates   2815806..2816843 (+) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain WP8-W18-ESBL-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2776587..2829346 2815806..2816843 within 0


Gene organization within MGE regions


Location: 2776587..2829346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R41_RS13110 (WP8W18E11_25790) brxC 2778553..2782233 (-) 3681 WP_182925313.1 BREX system P-loop protein BrxC -
  H7R41_RS13115 (WP8W18E11_25800) - 2782273..2782848 (-) 576 WP_002010699.1 DUF1788 domain-containing protein -
  H7R41_RS13120 (WP8W18E11_25810) - 2782865..2783476 (-) 612 WP_182925314.1 DUF1819 family protein -
  H7R41_RS13125 (WP8W18E11_25820) - 2783487..2784353 (-) 867 WP_182925315.1 YafY family protein -
  H7R41_RS13130 (WP8W18E11_25830) - 2784474..2785493 (-) 1020 WP_182925316.1 HNH endonuclease domain-containing protein -
  H7R41_RS13135 (WP8W18E11_25840) - 2785486..2786070 (-) 585 WP_182925317.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  H7R41_RS13140 (WP8W18E11_25850) - 2786140..2786370 (-) 231 WP_182925318.1 AlpA family transcriptional regulator -
  H7R41_RS13145 (WP8W18E11_25860) - 2786463..2787356 (-) 894 WP_182925319.1 hydrolase or metal-binding protein -
  H7R41_RS13150 (WP8W18E11_25870) - 2787470..2788513 (-) 1044 WP_182925320.1 YqaJ viral recombinase family protein -
  H7R41_RS13155 (WP8W18E11_25880) - 2788595..2788810 (-) 216 WP_182925321.1 hypothetical protein -
  H7R41_RS13160 (WP8W18E11_25890) - 2788908..2789942 (-) 1035 WP_182925420.1 DUF932 domain-containing protein -
  H7R41_RS13165 (WP8W18E11_25900) - 2790017..2790322 (-) 306 WP_182925421.1 hypothetical protein -
  H7R41_RS13170 (WP8W18E11_25910) - 2790441..2790821 (-) 381 WP_182925322.1 hypothetical protein -
  H7R41_RS13175 (WP8W18E11_25920) - 2790833..2791402 (-) 570 WP_182925323.1 hypothetical protein -
  H7R41_RS13180 (WP8W18E11_25930) - 2791420..2791680 (-) 261 WP_182925324.1 hypothetical protein -
  H7R41_RS13185 (WP8W18E11_25940) - 2792274..2793236 (-) 963 WP_182925325.1 hypothetical protein -
  H7R41_RS13190 (WP8W18E11_25950) - 2793319..2794296 (-) 978 WP_227552539.1 inovirus-type Gp2 protein -
  H7R41_RS13195 (WP8W18E11_25960) - 2794785..2796284 (-) 1500 WP_182925326.1 hypothetical protein -
  H7R41_RS13200 (WP8W18E11_25970) - 2796415..2797116 (+) 702 WP_182925327.1 hypothetical protein -
  H7R41_RS13205 (WP8W18E11_25980) - 2797126..2798268 (-) 1143 WP_180183011.1 phage integrase central domain-containing protein -
  H7R41_RS13215 (WP8W18E11_25990) ggt 2798976..2800961 (-) 1986 WP_005135733.1 gamma-glutamyltransferase -
  H7R41_RS13220 (WP8W18E11_26000) - 2801265..2802788 (-) 1524 WP_000857089.1 DHA2 family efflux MFS transporter permease subunit -
  H7R41_RS13225 (WP8W18E11_26010) - 2802795..2803946 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  H7R41_RS13230 (WP8W18E11_26020) - 2804216..2804713 (-) 498 WP_005135734.1 thioesterase family protein -
  H7R41_RS13235 (WP8W18E11_26030) hemB 2804856..2805869 (+) 1014 WP_000222569.1 porphobilinogen synthase -
  H7R41_RS13240 (WP8W18E11_26040) - 2805895..2807010 (+) 1116 WP_000546689.1 FAD-binding oxidoreductase -
  H7R41_RS13245 (WP8W18E11_26050) - 2807002..2807907 (-) 906 WP_001039371.1 TIGR01777 family oxidoreductase -
  H7R41_RS13250 (WP8W18E11_26060) - 2807996..2808448 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  H7R41_RS13255 (WP8W18E11_26070) - 2808828..2809205 (+) 378 WP_001059437.1 VOC family protein -
  H7R41_RS13260 (WP8W18E11_26080) - 2809273..2809746 (-) 474 WP_000152335.1 OsmC family protein -
  H7R41_RS13265 (WP8W18E11_26090) - 2809911..2811179 (+) 1269 WP_002042946.1 exonuclease SbcCD subunit D -
  H7R41_RS13270 (WP8W18E11_26100) - 2811189..2814785 (+) 3597 WP_000698794.1 SbcC/MukB-like Walker B domain-containing protein -
  H7R41_RS13275 (WP8W18E11_26110) - 2814987..2815679 (-) 693 WP_001108509.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R41_RS13280 (WP8W18E11_26120) pilT 2815806..2816843 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  H7R41_RS13285 (WP8W18E11_26130) pilU 2816871..2817989 (+) 1119 WP_114150255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7R41_RS13290 (WP8W18E11_26140) fur 2818059..2818496 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  H7R41_RS13295 (WP8W18E11_26150) - 2818608..2819006 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  H7R41_RS13300 (WP8W18E11_26160) - 2819061..2819384 (-) 324 WP_000442590.1 RnfH family protein -
  H7R41_RS13305 (WP8W18E11_26170) - 2819384..2820451 (-) 1068 WP_004748097.1 hypothetical protein -
  H7R41_RS13310 (WP8W18E11_26180) - 2820604..2821059 (-) 456 WP_000782976.1 bacteriohemerythrin -
  H7R41_RS13315 (WP8W18E11_26190) - 2821312..2822160 (-) 849 WP_000336554.1 class II glutamine amidotransferase -
  H7R41_RS13320 (WP8W18E11_26200) - 2822269..2823051 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  H7R41_RS13325 (WP8W18E11_26210) argB 2823172..2824080 (-) 909 WP_001135419.1 acetylglutamate kinase -
  H7R41_RS13330 (WP8W18E11_26220) - 2824095..2825513 (-) 1419 WP_001102843.1 phosphomannomutase/phosphoglucomutase -
  H7R41_RS13335 (WP8W18E11_26230) dut 2825713..2826165 (-) 453 WP_000868152.1 dUTP diphosphatase -
  H7R41_RS13340 (WP8W18E11_26240) rep 2826191..2828230 (-) 2040 WP_000093035.1 DNA helicase Rep -
  H7R41_RS13345 (WP8W18E11_26250) - 2828424..2829077 (-) 654 WP_001202415.1 OmpA family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=77311 H7R41_RS13280 WP_000355489.1 2815806..2816843(+) (pilT) [Acinetobacter baumannii strain WP8-W18-ESBL-11]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=77311 H7R41_RS13280 WP_000355489.1 2815806..2816843(+) (pilT) [Acinetobacter baumannii strain WP8-W18-ESBL-11]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCCCATGAAATTATGATTGGTATTCCGGCTATTCGTAATTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTAGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGTCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment