Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7K58_RS19350 Genome accession   NZ_AP022188
Coordinates   4174109..4175143 (+) Length   344 a.a.
NCBI ID   WP_041206426.1    Uniprot ID   -
Organism   Aeromonas media strain WP7-W18-ESBL-02     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4169109..4180143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K58_RS19310 (WP7W18E02_37420) - 4169327..4169701 (+) 375 WP_005332296.1 YacL family protein -
  H7K58_RS19315 (WP7W18E02_37430) - 4169752..4170048 (+) 297 WP_005332293.1 hypothetical protein -
  H7K58_RS19320 (WP7W18E02_37440) - 4170244..4171074 (+) 831 WP_182786636.1 CPBP family intramembrane glutamic endopeptidase -
  H7K58_RS19325 (WP7W18E02_37450) - 4171153..4171572 (-) 420 WP_041206420.1 DUF4426 domain-containing protein -
  H7K58_RS19330 (WP7W18E02_37460) yggU 4171577..4171885 (-) 309 WP_043128939.1 DUF167 family protein YggU -
  H7K58_RS19335 (WP7W18E02_37470) - 4171885..4172436 (-) 552 WP_025325667.1 YggT family protein -
  H7K58_RS19340 (WP7W18E02_37480) proC 4172460..4173284 (-) 825 WP_182786638.1 pyrroline-5-carboxylate reductase -
  H7K58_RS19345 (WP7W18E02_37490) - 4173374..4174069 (-) 696 WP_182786640.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7K58_RS19350 (WP7W18E02_37500) pilT 4174109..4175143 (+) 1035 WP_041206426.1 type IVa pilus ATPase TapT Machinery gene
  H7K58_RS19355 (WP7W18E02_37510) pilU 4175170..4176279 (+) 1110 WP_042649788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7K58_RS19360 (WP7W18E02_37520) yaaA 4176312..4177085 (+) 774 WP_043128932.1 peroxide stress protein YaaA -
  H7K58_RS19365 (WP7W18E02_37530) srmB 4177271..4178494 (-) 1224 WP_182786642.1 ATP-dependent RNA helicase SrmB -
  H7K58_RS19370 (WP7W18E02_37540) - 4178623..4179333 (+) 711 WP_182786644.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38257.00 Da        Isoelectric Point: 6.6958

>NTDB_id=77014 H7K58_RS19350 WP_041206426.1 4174109..4175143(+) (pilT) [Aeromonas media strain WP7-W18-ESBL-02]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDYQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLEDLEAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVAALDARAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=77014 H7K58_RS19350 WP_041206426.1 4174109..4175143(+) (pilT) [Aeromonas media strain WP7-W18-ESBL-02]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCAGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGAGAGGTTCGCAAGATCAATTTACCCGCCCTGGATCACCGTGAAGTGCACGCCCTGATCTACGACA
TCATGAACGACTATCAGCGCAAGGAGCTGGAGGAGAATTTCGAGGTGGACTTCTCCTTCGAGGTACCCGGCATGGCGCGT
TTTCGGGTCAACGCCTTTCAACAGGCGCGGGGCTCTGGCGCCGTGTTTCGCACCATACCCAGTACCGTTCTGACCCTGGA
GGATCTGGAAGCTCCCGAGATGTTCCGCAAGATAGCCGAGTTTCCGCGCGGCCTGGTGCTGGTCACGGGCCCGACAGGTT
CAGGCAAGTCCACCACGCTTGCGGCCATGGTCAATTACATCAATGACAACTTCCACCACCACATCCTCACCATTGAGGAT
CCCATCGAGTTCGTACACGAGAACAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCACCGCGATACCAAGAGTTTCGCCAA
CGCCCTTCGCTCGGCCCTGCGTGAGGACCCGGACATCATACTGGTGGGTGAAATGCGCGACCTCGAGACCATCCGCCTCG
CCATGACGGCGGCAGAGACCGGTCATCTGGTATTCGGTACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGAGATCCATGCTCTCAGAGTCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGTGGCCGGGTGGCAGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTCGCCCAGCTCTACTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTCGTCAACCGGGGCGTGGTTGCCGCCCTCGATGCCAGGGCCAAGGCGGCCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

76.968

99.709

0.767
  pilT Acinetobacter baumannii strain A118

76.968

99.709

0.767
  pilT Acinetobacter nosocomialis M2

76.968

99.709

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.744

100

0.767
  pilT Pseudomonas aeruginosa PAK

75.872

100

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.558

97.965

0.75
  pilT Vibrio cholerae strain A1552

76.558

97.965

0.75
  pilT Legionella pneumophila strain Lp02

72.727

95.93

0.698
  pilT Legionella pneumophila strain ERS1305867

72.727

95.93

0.698
  pilT Neisseria meningitidis 8013

68.696

100

0.689
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

43.478

100

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment