Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7K58_RS19355 Genome accession   NZ_AP022188
Coordinates   4175170..4176279 (+) Length   369 a.a.
NCBI ID   WP_042649788.1    Uniprot ID   -
Organism   Aeromonas media strain WP7-W18-ESBL-02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4170170..4181279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K58_RS19320 (WP7W18E02_37440) - 4170244..4171074 (+) 831 WP_182786636.1 CPBP family intramembrane glutamic endopeptidase -
  H7K58_RS19325 (WP7W18E02_37450) - 4171153..4171572 (-) 420 WP_041206420.1 DUF4426 domain-containing protein -
  H7K58_RS19330 (WP7W18E02_37460) yggU 4171577..4171885 (-) 309 WP_043128939.1 DUF167 family protein YggU -
  H7K58_RS19335 (WP7W18E02_37470) - 4171885..4172436 (-) 552 WP_025325667.1 YggT family protein -
  H7K58_RS19340 (WP7W18E02_37480) proC 4172460..4173284 (-) 825 WP_182786638.1 pyrroline-5-carboxylate reductase -
  H7K58_RS19345 (WP7W18E02_37490) - 4173374..4174069 (-) 696 WP_182786640.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7K58_RS19350 (WP7W18E02_37500) pilT 4174109..4175143 (+) 1035 WP_041206426.1 type IVa pilus ATPase TapT Machinery gene
  H7K58_RS19355 (WP7W18E02_37510) pilU 4175170..4176279 (+) 1110 WP_042649788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7K58_RS19360 (WP7W18E02_37520) yaaA 4176312..4177085 (+) 774 WP_043128932.1 peroxide stress protein YaaA -
  H7K58_RS19365 (WP7W18E02_37530) srmB 4177271..4178494 (-) 1224 WP_182786642.1 ATP-dependent RNA helicase SrmB -
  H7K58_RS19370 (WP7W18E02_37540) - 4178623..4179333 (+) 711 WP_182786644.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  H7K58_RS19375 (WP7W18E02_37550) brnQ 4179525..4180832 (+) 1308 WP_041207086.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40914.01 Da        Isoelectric Point: 6.0462

>NTDB_id=77015 H7K58_RS19355 WP_042649788.1 4175170..4176279(+) (pilU) [Aeromonas media strain WP7-W18-ESBL-02]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVPLEGGPLDRQAALALVRDSLDETHFERYLHSREANYAIHREGLGR
FRVSAFWQQDMPGMVLRRIETRIPTFEELVLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTDSFDVALKNSMRQAPDVILIGEIRTQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPEAKHRQFLFDLSFNLRAIVAQQLLPSVNGSRRIAAFEILLNTPLITDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=77015 H7K58_RS19355 WP_042649788.1 4175170..4176279(+) (pilU) [Aeromonas media strain WP7-W18-ESBL-02]
ATGAACATGGATGTTCTGCTCGCTGCACTGGTTGAACAAAAGGGATCGGATCTCTTCATCACGGTCGATGCCCCCCCCAC
CCTCAAGGTCAATGGACGCCTGGTACCGCTCGAGGGGGGGCCGCTGGACAGGCAGGCGGCGCTGGCCCTGGTCAGGGATA
GCCTGGACGAGACGCACTTCGAGCGATACCTGCATAGCCGGGAGGCAAACTACGCCATCCACCGCGAAGGACTTGGCCGA
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGTCGTATCGAGACCCGTATTCCCACCTTCGA
GGAGTTGGTACTGCCCCCCATCCTGCAGGATGTGGCCATGGCCAAACGCGGGTTGGTGCTGTTCGTCGGTGCCACGGGGG
CTGGCAAGTCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCACGGCGACGGCCACATACTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCATGGGCGCTGCCTGGTGACCCAGCGGGAGGTCGGCATAGACACCGACTCCTTCGACGT
GGCCCTCAAGAATTCCATGCGCCAGGCTCCGGATGTCATCCTGATCGGTGAGATCCGTACCCAGGAAACCATGGAACTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGGAGGCGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTGGCGCAGCA
ACTGCTCCCCAGCGTGAATGGCAGCCGCCGGATCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGCGAGATGCACAGGCTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGTCGCGAGCAGCTGGGTGCAGGCACACTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.783

99.729

0.596
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

56.374

95.664

0.539
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.39

89.702

0.371
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Pseudomonas aeruginosa PAK

38.889

92.683

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.889

92.683

0.36

Multiple sequence alignment